Overexpression of a rice BAHD acyltransferase gene in switchgrass (Panicum virgatum L.) enhances saccharification

Li, Guotian; Jones, Kyle C.; Eudes, Aymerick; Pidatala, Venkataramana R.; Sun, Jian; Xu, Feng; Zhang, Chengcheng; Wei, Tong; Jain, Rashmi; Birdseye, Devon; Canlas, Patrick E.; Baidoo, Edward E. K.; Duong, Phat Q.; Sharma, Manoj K.; Singh, Seema; Ruan, Deling; Keasling, Jay D.; Mortimer, Jenny C.; Loqué, Dominique; Bartley, Laura E.; Scheller, Henrik V.; Ronald, Pamela C.

doi:10.1186/s12896-018-0464-8

Title: Overexpression of a rice BAHD acyltransferase gene in switchgrass (Panicum virgatum L.) enhances saccharification

Journal Article · Tue Sep 04 00:00:00 EDT 2018 · BMC Biotechnology (Online)

DOI:https://doi.org/10.1186/s12896-018-0464-8· OSTI ID:1618881

Li, Guotian; Jones, Kyle C.; Eudes, Aymerick; Pidatala, Venkataramana R.; Sun, Jian; Xu, Feng; Zhang, Chengcheng; Wei, Tong; Jain, Rashmi; Birdseye, Devon; Canlas, Patrick E.; Baidoo, Edward E. K.; Duong, Phat Q.; Sharma, Manoj K.; Singh, Seema; Ruan, Deling; Keasling, Jay D.; Mortimer, Jenny C.; Loqué, Dominique; Bartley, Laura E. more »

Background: Switchgrass (Panicum virgatum L.) is a promising bioenergy feedstock because it can be grown on marginal land and produces abundant biomass. Recalcitrance of the lignocellulosic components of the switchgrass cell wall to enzymatic degradation into simple sugars impedes efficient biofuel production. We previously demonstrated that overexpression of OsAT10, a BAHD acyltransferase gene, enhances saccharification efficiency in rice. Results: Here we show that overexpression of the rice OsAT10 gene in switchgrass decreased the levels of cell wall-bound ferulic acid (FA) in green leaf tissues and to a lesser extent in senesced tissues, and significantly increased levels of cell wall-bound p-coumaric acid (p-CA) in green leaves but decreased its level in senesced tissues of the T0plants under greenhouse conditions. The engineered switchgrass lines exhibit an approximate 40% increase in saccharification efficiency in green tissues and a 30% increase in senesced tissues. Conclusion: Our study demonstrates that overexpression of OsAT10, a rice BAHD acyltransferase gene, enhances saccharification of lignocellulosic biomass in switchgrass.

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Research Organization:: Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER)

Grant/Contract Number:: AC02-05CH11231; SC006904

OSTI ID:: 1618881

Alternate ID(s):: OSTI ID: 1477379

Journal Information:: BMC Biotechnology (Online), Journal Name: BMC Biotechnology (Online) Vol. 18 Journal Issue: 1; ISSN 1472-6750

Publisher:: Springer Science + Business MediaCopyright Statement

Country of Publication:: United Kingdom

Language:: English

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Cited by: 34 works

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