Performance of three delignifying pretreatments on hardwoods: hydrolysis yields, comprehensive mass balances, and lignin properties

Bhalla, Aditya; Cai, Charles M.; Xu, Feng; Singh, Sandip K.; Bansal, Namita; Phongpreecha, Thanaphong; Dutta, Tanmoy; Foster, Cliff E.; Kumar, Rajeev; Simmons, Blake A.; Singh, Seema; Wyman, Charles E.; Hegg, Eric L.; Hodge, David B.

doi:10.1186/s13068-019-1546-0

Title: Performance of three delignifying pretreatments on hardwoods: hydrolysis yields, comprehensive mass balances, and lignin properties

Journal Article · Mon Sep 09 00:00:00 EDT 2019 · Biotechnology for Biofuels

DOI:https://doi.org/10.1186/s13068-019-1546-0· OSTI ID:1618770

Bhalla, Aditya; Cai, Charles M.; Xu, Feng; Singh, Sandip K.; Bansal, Namita; Phongpreecha, Thanaphong; Dutta, Tanmoy; Foster, Cliff E.; Kumar, Rajeev; Simmons, Blake A.; Singh, Seema; Wyman, Charles E.; Hegg, Eric L.;

Abstract Background In this work, three pretreatments under investigation at the DOE Bioenergy Research Centers (BRCs) were subjected to a side-by-side comparison to assess their performance on model bioenergy hardwoods (a eucalyptus and a hybrid poplar). These include co-solvent-enhanced lignocellulosic fractionation (CELF), pretreatment with an ionic liquid using potentially biomass-derived components (cholinium lysinate or [Ch][Lys]), and two-stage Cu-catalyzed alkaline hydrogen peroxide pretreatment (Cu-AHP). For each of the feedstocks, the pretreatments were assessed for their impact on lignin and xylan solubilization and enzymatic hydrolysis yields as a function of enzyme loading. Lignins recovered from the pretreatments were characterized for polysaccharide content, molar mass distributions, β-aryl ether content, and response to depolymerization by thioacidolysis. Results All three pretreatments resulted in significant solubilization of lignin and xylan, with the CELF pretreatment solubilizing the majority of both biopolymer categories. Enzymatic hydrolysis yields were shown to exhibit a strong, positive correlation with the lignin solubilized for the low enzyme loadings. The pretreatment-derived solubles in the [Ch][Lys]-pretreated biomass were presumed to contribute to inhibition of enzymatic hydrolysis in the eucalyptus as a substantial fraction of the pretreatment liquor was carried forward into hydrolysis for this pretreatment. The pretreatment-solubilized lignins exhibited significant differences in polysaccharide content, molar mass distributions, aromatic monomer yield by thioacidolysis, and β-aryl ether content. Key trends include a substantially higher polysaccharide content in the lignins recovered from the [Ch][Lys] pretreatment and high β-aryl ether contents and aromatic monomer yields from the Cu-AHP pretreatment. For all lignins, the ¹³ C NMR-determined β-aryl ether content was shown to be correlated with the monomer yield with a second-order functionality. Conclusions Overall, it was demonstrated that the three pretreatments highlighted in this study demonstrated uniquely different functionalities in reducing biomass recalcitrance and achieving higher enzymatic hydrolysis yields for the hybrid poplar while yielding a lignin-rich stream that may be suitable for valorization. Furthermore, modification of lignin during pretreatment, particularly cleavage of β-aryl ether bonds, is shown to be detrimental to subsequent depolymerization.

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Research Organization:: Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER)

Grant/Contract Number:: FC02-07ER64494; AC05-00OR22725; DOE BER DE-AC02-05CH11231; SC0018409; AC02-05CH11231

OSTI ID:: 1618770

Alternate ID(s):: OSTI ID: 1572794

Journal Information:: Biotechnology for Biofuels, Journal Name: Biotechnology for Biofuels Vol. 12 Journal Issue: 1; ISSN 1754-6834

Publisher:: Springer Science + Business MediaCopyright Statement

Country of Publication:: Netherlands

Language:: English

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Cited by: 20 works

Citation information provided by
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References (71)

Growth Variation Among Hybrid Poplar Varieties in Michigan, USA and the Implications for Commercial Biomass Production. Miller, Raymond O. BioEnergy Research, Vol. 11, Issue 4 https://doi.org/10.1007/s12155-018-9937-9	journal	August 2018
Effective alkaline metal-catalyzed oxidative delignification of hybrid poplar Bhalla, Aditya; Bansal, Namita; Stoklosa, Ryan J. Biotechnology for Biofuels, Vol. 9, Issue 1 https://doi.org/10.1186/s13068-016-0442-0	journal	February 2016
Cell wall-associated transition metals improve alkaline-oxidative pretreatment in diverse hardwoods Bansal, Namita; Bhalla, Aditya; Pattathil, Sivakumar Green Chemistry, Vol. 18, Issue 5 https://doi.org/10.1039/C5GC01748C	journal	January 2016
Brazil considers transgenic trees Ledford, Heidi Nature, Vol. 512, Issue 7515 https://doi.org/10.1038/512357a	journal	August 2014
Chemical Transformations of Poplar Lignin during Cosolvent Enhanced Lignocellulosic Fractionation Process Meng, Xianzhi; Parikh, Aakash; Seemala, Bhogeswararao ACS Sustainable Chemistry & Engineering, Vol. 6, Issue 7 https://doi.org/10.1021/acssuschemeng.8b01028	journal	May 2018
Transforming biomass conversion with ionic liquids: process intensification and the development of a high-gravity, one-pot process for the production of cellulosic ethanol Xu, Feng; Sun, Jian; Konda, N. V. S. N. Murthy Energy & Environmental Science, Vol. 9, Issue 3 https://doi.org/10.1039/C5EE02940F	journal	January 2016
Selective Degradation of Wood Lignin over Noble‐Metal Catalysts in a Two‐Step Process Yan, Ning; Zhao, Chen; Dyson, Paul J. ChemSusChem, Vol. 1, Issue 7 https://doi.org/10.1002/cssc.200800080	journal	July 2008
Pretreatment of lignocellulose: Formation of inhibitory by-products and strategies for minimizing their effects Jönsson, Leif J.; Martín, Carlos Bioresource Technology, Vol. 199 https://doi.org/10.1016/j.biortech.2015.10.009	journal	January 2016
Temperature-dependent phase behaviour of tetrahydrofuran–water alters solubilization of xylan to improve co-production of furfurals from lignocellulosic biomass Smith, Micholas Dean; Cai, Charles M.; Cheng, Xiaolin Green Chemistry, Vol. 20, Issue 7 https://doi.org/10.1039/C7GC03608F	journal	January 2018
Bright Side of Lignin Depolymerization: Toward New Platform Chemicals Sun, Zhuohua; Fridrich, Bálint; de Santi, Alessandra Chemical Reviews, Vol. 118, Issue 2 https://doi.org/10.1021/acs.chemrev.7b00588	journal	January 2018
Molar mass determination of lignins by size-exclusion chromatography: towards standardisation of the method Baumberger, Stéphanie; Abaecherli, Alfred; Fasching, Mario Holzforschung, Vol. 61, Issue 4 https://doi.org/10.1515/HF.2007.074	journal	June 2007
Inside Cover: Co-solvent Pretreatment Reduces Costly Enzyme Requirements for High Sugar and Ethanol Yields from Lignocellulosic Biomass (ChemSusChem 10/2015) Nguyen, Thanh Yen; Cai, Charles M.; Kumar, Rajeev ChemSusChem, Vol. 8, Issue 10 https://doi.org/10.1002/cssc.201500515	journal	May 2015
Activation of lignocellulosic biomass for higher sugar yields using aqueous ionic liquid at low severity process conditions Parthasarathi, Ramakrishnan; Sun, Jian; Dutta, Tanmoy Biotechnology for Biofuels, Vol. 9, Issue 1 https://doi.org/10.1186/s13068-016-0561-7	journal	August 2016
Catalysis with Cu ^II (bpy) improves alkaline hydrogen peroxide pretreatment Li, Zhenglun; Chen, Charles H.; Liu, Tongjun Biotechnology and Bioengineering, Vol. 110, Issue 4 https://doi.org/10.1002/bit.24793	journal	December 2012
Replacing 100% of phenol in phenolic adhesive formulations with lignin: ARTICLE Kalami, Somayyeh; Arefmanesh, Maryam; Master, Emma Journal of Applied Polymer Science, Vol. 134, Issue 30 https://doi.org/10.1002/app.45124	journal	April 2017
Cellulosic ethanol: status and innovation Lynd, Lee R.; Liang, Xiaoyu; Biddy, Mary J. Current Opinion in Biotechnology, Vol. 45 https://doi.org/10.1016/j.copbio.2017.03.008	journal	June 2017
Efficient biomass pretreatment using ionic liquids derived from lignin and hemicellulose Socha, A. M.; Parthasarathi, R.; Shi, J. Proceedings of the National Academy of Sciences, Vol. 111, Issue 35 https://doi.org/10.1073/pnas.1405685111	journal	August 2014
Deconstruction of hybrid poplar to monomeric sugars and aromatics using ethanol organosolv fractionation Bär, Janosch; Phongpreecha, Thanaphong; Singh, Sandip Kumar Biomass Conversion and Biorefinery, Vol. 8, Issue 4 https://doi.org/10.1007/s13399-018-0330-x	journal	August 2018
The Sankey Diagram in Energy and Material Flow Management: Part I: History Schmidt, Mario Journal of Industrial Ecology, Vol. 12, Issue 1 https://doi.org/10.1111/j.1530-9290.2008.00004.x	journal	February 2008
Chemical and structural changes associated with Cu-catalyzed alkaline-oxidative delignification of hybrid poplar Li, Zhenglun; Bansal, Namita; Azarpira, Ali Biotechnology for Biofuels, Vol. 8, Issue 1 https://doi.org/10.1186/s13068-015-0300-5	journal	August 2015
Comparative data on effects of leading pretreatments and enzyme loadings and formulations on sugar yields from different switchgrass sources Wyman, Charles E.; Balan, Venkatesh; Dale, Bruce E. Bioresource Technology, Vol. 102, Issue 24 https://doi.org/10.1016/j.biortech.2011.06.069	journal	December 2011
Comparison of Different Biomass Pretreatment Techniques and Their Impact on Chemistry and Structure Singh, Seema; Cheng, Gang; Sathitsuksanoh, Noppadon Frontiers in Energy Research, Vol. 2 https://doi.org/10.3389/fenrg.2014.00062	journal	February 2015
Extraction, Recovery, and Characterization of Hardwood and Grass Hemicelluloses for Integration into Biorefining Processes Stoklosa, Ryan J.; Hodge, David B. Industrial & Engineering Chemistry Research, Vol. 51, Issue 34 https://doi.org/10.1021/ie301260w	journal	August 2012
Solubility of Lignin and Acetylated Lignin in Organic Solvents Sameni, Javad; Krigstin, Sally; Sain, Mohini BioResources, Vol. 12, Issue 1 https://doi.org/10.15376/biores.12.1.1548-1565	journal	November 2016
CELF pretreatment of corn stover boosts ethanol titers and yields from high solids SSF with low enzyme loadings Nguyen, Thanh Yen; Cai, Charles M.; Osman, Omar Green Chemistry, Vol. 18, Issue 6 https://doi.org/10.1039/C5GC01977J	journal	January 2016
2016 Billion-Ton Report: Advancing Domestic Resources for a Thriving Bioeconomy Langholtz, M. H.; Stokes, B. J.; Eaton, L. M. https://doi.org/10.2172/1271651 DOE/EE-1440	report	July 2016
Comparison of enzymatic reactivity of corn stover solids prepared by dilute acid, AFEX™, and ionic liquid pretreatments Gao, Xiadi; Kumar, Rajeev; Singh, Seema Biotechnology for Biofuels, Vol. 7, Issue 1 https://doi.org/10.1186/1754-6834-7-71	journal	January 2014
Rapid and effective oxidative pretreatment of woody biomass at mild reaction conditions and low oxidant loadings Li, Zhenglun; Chen, Charles H.; Hegg, Eric L. Biotechnology for Biofuels, Vol. 6, Issue 1 https://doi.org/10.1186/1754-6834-6-119	journal	January 2013
The impact of lignin source on its self-assembly in solution Ratnaweera, Dilru R.; Saha, Dipendu; Pingali, Sai Venkatesh RSC Advances, Vol. 5, Issue 82 https://doi.org/10.1039/C5RA13485D	journal	January 2015
Effect of complete dissolution in LiCl/DMSO on the isolation and characteristics of lignin from wheat straw internode Gu, Feng; Wu, Wenjuan; Wang, Zhiguo Industrial Crops and Products, Vol. 74 https://doi.org/10.1016/j.indcrop.2015.06.002	journal	November 2015
A Multifunctional Cosolvent Pair Reveals Molecular Principles of Biomass Deconstruction Patri, Abhishek S.; Mostofian, Barmak; Pu, Yunqiao Journal of the American Chemical Society, Vol. 141, Issue 32 https://doi.org/10.1021/jacs.8b10242	journal	July 2019
Impacts of delignification and hot water pretreatment on the water induced cell wall swelling behavior of grasses and its relation to cellulolytic enzyme hydrolysis and binding Williams, Daniel L.; Hodge, David B. Cellulose, Vol. 21, Issue 1 https://doi.org/10.1007/s10570-013-0149-3	journal	December 2013
Opportunities and Challenges in the Design and Analysis of Biomass Supply Chains Lautala, Pasi T.; Hilliard, Michael R.; Webb, Erin Environmental Management, Vol. 56, Issue 6 https://doi.org/10.1007/s00267-015-0565-2	journal	June 2015
Impact of mixed feedstocks and feedstock densification on ionic liquid pretreatment efficiency Shi, Jian; Thompson, Vicki S.; Yancey, Neal A. Biofuels, Vol. 4, Issue 1 https://doi.org/10.4155/bfs.12.82	journal	January 2013
A comparative study of ethanol production using dilute acid, ionic liquid and AFEX™ pretreated corn stover Uppugundla, Nirmal; da Costa Sousa, Leonardo; Chundawat, Shishir PS Biotechnology for Biofuels, Vol. 7, Issue 1 https://doi.org/10.1186/1754-6834-7-72	journal	January 2014
Novel renewable ionic liquids as highly effective solvents for pretreatment of rice straw biomass by selective removal of lignin Hou, Xue-Dan; Smith, Thomas J.; Li, Ning Biotechnology and Bioengineering, Vol. 109, Issue 10 https://doi.org/10.1002/bit.24522	journal	April 2012
Effect of xylan and lignin removal by batch and flowthrough pretreatment on the enzymatic digestibility of corn stover cellulose Yang, Bin; Wyman, Charles E. Biotechnology and Bioengineering, Vol. 86, Issue 1 https://doi.org/10.1002/bit.20043	journal	January 2004
Lignins: Natural polymers from oxidative coupling of 4-hydroxyphenyl- propanoids Ralph, John; Lundquist, Knut; Brunow, Gösta Phytochemistry Reviews, Vol. 3, Issue 1-2, p. 29-60 https://doi.org/10.1023/B:PHYT.0000047809.65444.a4	journal	January 2004
Lignin Esters for Use in Unsaturated Thermosets: Lignin Modification and Solubility Modeling Thielemans, Wim; Wool, Richard P. Biomacromolecules, Vol. 6, Issue 4 https://doi.org/10.1021/bm0500345	journal	July 2005
Milled Wood Lignin: A Linear Oligomer Crestini, Claudia; Melone, Federica; Sette, Marco Biomacromolecules, Vol. 12, Issue 11 https://doi.org/10.1021/bm200948r	journal	November 2011
Predicting lignin depolymerization yields from quantifiable properties using fractionated biorefinery lignins Phongpreecha, Thanaphong; Hool, Nicholas C.; Stoklosa, Ryan J. Green Chem., Vol. 19, Issue 21 https://doi.org/10.1039/C7GC02023F	journal	January 2017
The effect of delignification of forest biomass on enzymatic hydrolysis Yu, Zhiying; Jameel, Hasan; Chang, Hou-min Bioresource Technology, Vol. 102, Issue 19 https://doi.org/10.1016/j.biortech.2011.07.001	journal	October 2011
Structural analysis of hardwood native lignins by quantitative 13C NMR spectroscopy Balakshin, Mikhail Yu.; Capanema, Ewellyn A.; Santos, Ricardo B. Holzforschung, Vol. 70, Issue 2 https://doi.org/10.1515/hf-2014-0328	journal	February 2016
Ethanol Can Contribute to Energy and Environmental Goals Farrell, A. E. Science, Vol. 311, Issue 5760 https://doi.org/10.1126/science.1121416	journal	January 2006
Cosolvent pretreatment in cellulosic biofuel production: effect of tetrahydrofuran-water on lignin structure and dynamics Smith, Micholas Dean; Mostofian, Barmak; Cheng, Xiaolin Green Chemistry, Vol. 18, Issue 5 https://doi.org/10.1039/C5GC01952D	journal	January 2016
Summary of findings from the Biomass Refining Consortium for Applied Fundamentals and Innovation (CAFI): corn stover pretreatment Elander, Richard T.; Dale, Bruce E.; Holtzapple, Mark Cellulose, Vol. 16, Issue 4 https://doi.org/10.1007/s10570-009-9308-y	journal	June 2009
Linking Plant Biology and Pretreatment: Understanding the Structure and Organization of the Plant Cell Wall and Interactions with Cellulosic Biofuel Production Ong, Rebecca Garlock; Chundawat, Shishir P. S.; Hodge, David B. Plants and BioEnergy https://doi.org/10.1007/978-1-4614-9329-7_14	book	November 2013
Effect of liquid hot water pretreatment severity on properties of hardwood lignin and enzymatic hydrolysis of cellulose: Lignin Effect on Enzyme Hydrolysis of Cellulose Ko, Ja Kyong; Kim, Youngmi; Ximenes, Eduardo Biotechnology and Bioengineering, Vol. 112, Issue 2 https://doi.org/10.1002/bit.25349	journal	October 2014
Comparison of Dilute Acid and Sulfite Pretreatments on Acacia confusa for Biofuel Application and the Influence of Its Extractives Yeh, Ting-Feng; Chang, Mao-Ju; Chang, Wan-Jung Journal of Agricultural and Food Chemistry, Vol. 62, Issue 44 https://doi.org/10.1021/jf504461c	journal	October 2014
Fractionation and Improved Enzymatic Deconstruction of Hardwoods with Alkaline Delignification Stoklosa, Ryan J.; Hodge, David B. BioEnergy Research, Vol. 8, Issue 3 https://doi.org/10.1007/s12155-015-9579-0	journal	January 2015
The Catalytic Valorization of Lignin for the Production of Renewable Chemicals Zakzeski, Joseph; Bruijnincx, Pieter C. A.; Jongerius, Anna L. Chemical Reviews, Vol. 110, Issue 6, p. 3552-3599 https://doi.org/10.1021/cr900354u	journal	June 2010
Characterization of the Molar Masses of Hemicelluloses from Wood and Pulps Employing Size Exclusion Chromatography and Matrix-Assisted Laser Desorption Ionization Time-of-Flight Mass Spectrometry Jacobs, Anna; Dahlman, Olof Biomacromolecules, Vol. 2, Issue 3 https://doi.org/10.1021/bm010050b	journal	September 2001
Understanding pretreatment efficacy of four cholinium and imidazolium ionic liquids by chemistry and computation Sun, Ning; Parthasarathi, Ramakrishnan; Socha, Aaron M. Green Chem., Vol. 16, Issue 5 https://doi.org/10.1039/C3GC42401D	journal	January 2014
Antifungal activities of wood extractives Valette, Nicolas; Perrot, Thomas; Sormani, Rodnay Fungal Biology Reviews, Vol. 31, Issue 3 https://doi.org/10.1016/j.fbr.2017.01.002	journal	June 2017
Aromatic Monomers by in Situ Conversion of Reactive Intermediates in the Acid-Catalyzed Depolymerization of Lignin Deuss, Peter J.; Scott, Martin; Tran, Fanny Journal of the American Chemical Society, Vol. 137, Issue 23 https://doi.org/10.1021/jacs.5b03693	journal	June 2015
Modifying solubility of polymeric xylan extracted from Eucalyptus grandis and sugarcane bagasse by suitable side chain removing enzymes Gomes, Katiana R.; Chimphango, Annie F. A.; Görgens, Johann F. Carbohydrate Polymers, Vol. 131 https://doi.org/10.1016/j.carbpol.2015.05.029	journal	October 2015
Lignin-based rigid polyurethane foams with improved biodegradation Cateto, Carolina A.; Barreiro, Maria F.; Ottati, Carolina Journal of Cellular Plastics, Vol. 50, Issue 1 https://doi.org/10.1177/0021955X13504774	journal	September 2013
Characterization of Lignin Streams during Bionic Liquid-Based Pretreatment from Grass, Hardwood, and Softwood Dutta, Tanmoy; Papa, Gabriella; Wang, Eileen ACS Sustainable Chemistry & Engineering, Vol. 6, Issue 3 https://doi.org/10.1021/acssuschemeng.7b02991	journal	January 2018
Bioconversion of hybrid poplar to ethanol and co-products using an organosolv fractionation process: Optimization of process yields Pan, Xuejun; Gilkes, Neil; Kadla, John Biotechnology and Bioengineering, Vol. 94, Issue 5, p. 851-861 https://doi.org/10.1002/bit.20905	journal	January 2006
Comparative sugar recovery and fermentation data following pretreatment of poplar wood by leading technologies Wyman, Charles E.; Dale, Bruce E.; Elander, Richard T. Biotechnology Progress, Vol. 25, Issue 2 https://doi.org/10.1002/btpr.142	journal	March 2009
Characterization of milled wood lignin and ethanol organosolv lignin from miscanthus El Hage, Roland; Brosse, Nicolas; Chrusciel, Laurent Polymer Degradation and Stability, Vol. 94, Issue 10 https://doi.org/10.1016/j.polymdegradstab.2009.07.007	journal	October 2009
MOLECULAR PROPERTIES OF SIX 4-O-METHYLGLUCURONOXYLANS ¹ Goring, D. A. I.; Timell, T. E. The Journal of Physical Chemistry, Vol. 64, Issue 10 https://doi.org/10.1021/j100839a015	journal	October 1960
One-pot ionic liquid pretreatment and saccharification of switchgrass Shi, Jian; Gladden, John M.; Sathitsuksanoh, Noppadon Green Chemistry, Vol. 15, Issue 9 https://doi.org/10.1039/c3gc40545a	journal	January 2013
Coordinated development of leading biomass pretreatment technologies Wyman, Charles E.; Dale, Bruce E.; Elander, Richard T. Bioresource Technology, Vol. 96, Issue 18, p. 1959-1966 https://doi.org/10.1016/j.biortech.2005.01.010	journal	December 2005
Mechanism of Assembly of Xylan onto Cellulose Surfaces Linder, Åsa; Bergman, Rikard; Bodin, Aase Langmuir, Vol. 19, Issue 12 https://doi.org/10.1021/la0341355	journal	June 2003
Soluble and insoluble solids contributions to high-solids enzymatic hydrolysis of lignocellulose Hodge, David B.; Karim, M. Nazmul; Schell, Daniel J. Bioresource Technology, Vol. 99, Issue 18, p. 8940-8948 https://doi.org/10.1016/j.biortech.2008.05.015	journal	December 2008
A comparison of liquid hot water and steam pretreatments of sugar cane bagasse for bioconversion to ethanol Laser, Mark; Schulman, Deborah; Allen, Stephen G. Bioresource Technology, Vol. 81, Issue 1 https://doi.org/10.1016/S0960-8524(01)00103-1	journal	January 2002
Comparisons of SPORL and dilute acid pretreatments for sugar and ethanol productions from aspen Tian, S.; Zhu, W.; Gleisner, R. Biotechnology Progress, Vol. 27, Issue 2 https://doi.org/10.1002/btpr.545	journal	January 2011
Overcoming factors limiting high-solids fermentation of lignocellulosic biomass to ethanol Nguyen, Thanh Yen; Cai, Charles M.; Kumar, Rajeev Proceedings of the National Academy of Sciences, Vol. 114, Issue 44 https://doi.org/10.1073/pnas.1704652114	journal	October 2017
Short-rotation woody crops for bioenergy and biofuels applications Hinchee, Maud; Rottmann, William; Mullinax, Lauren In Vitro Cellular & Developmental Biology - Plant, Vol. 45, Issue 6 https://doi.org/10.1007/s11627-009-9235-5	journal	August 2009
The Sankey Diagram in Energy and Material Flow Management Schmidt, Mario Journal of Industrial Ecology, Vol. 12, Issue 2 https://doi.org/10.1111/j.1530-9290.2008.00015.x	journal	April 2008

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59 BASIC BIOLOGICAL SCIENCES
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MOESM1 of Performance of three delignifying pretreatments on hardwoods: hydrolysis yields, comprehensive mass balances, and lignin properties Bhalla, Aditya; Cai, Charles; Xu, Feng https://doi.org/10.6084/m9.figshare.9790601.v1 The current record is supplemented by this text	text	January 2019
Performance of three delignifying pretreatments on hardwoods: hydrolysis yields, comprehensive mass balances, and lignin properties Bhalla, Aditya; Cai, Charles; Xu, Feng https://doi.org/10.6084/m9.figshare.c.4660040 The current record is supplemented by this collection	collection	January 2019
Performance of three delignifying pretreatments on hardwoods: hydrolysis yields, comprehensive mass balances, and lignin properties Bhalla, Aditya; Cai, Charles; Xu, Feng https://doi.org/10.6084/m9.figshare.c.4660040.v1 The current record is supplemented by this collection	collection	January 2019
MOESM1 of Performance of three delignifying pretreatments on hardwoods: hydrolysis yields, comprehensive mass balances, and lignin properties Bhalla, Aditya; Cai, Charles; Xu, Feng https://doi.org/10.6084/m9.figshare.9790601 The current record is supplemented by this text	text	January 2019