Integrative visual omics of the white-rot fungus Polyporus brumalis exposes the biotechnological potential of its oxidative enzymes for delignifying raw plant biomass

Miyauchi, Shingo; Rancon, Anaïs; Drula, Elodie; Hage, Hayat; Chaduli, Delphine; Favel, Anne; Grisel, Sacha; Henrissat, Bernard; Herpoël-Gimbert, Isabelle; Ruiz-Dueñas, Francisco J.; Chevret, Didier; Hainaut, Matthieu; Lin, Junyan; Wang, Mei; Pangilinan, Jasmyn; Lipzen, Anna; Lesage-Meessen, Laurence; Navarro, David; Riley, Robert; Grigoriev, Igor V.; Zhou, Simeng; Raouche, Sana; Rosso, Marie-Noëlle

doi:10.1186/s13068-018-1198-5

Title: Integrative visual omics of the white-rot fungus Polyporus brumalis exposes the biotechnological potential of its oxidative enzymes for delignifying raw plant biomass

Journal Article · Mon Jul 23 00:00:00 EDT 2018 · Biotechnology for Biofuels

DOI:https://doi.org/10.1186/s13068-018-1198-5· OSTI ID:1618746

Miyauchi, Shingo; Rancon, Anaïs; Drula, Elodie; Hage, Hayat; Chaduli, Delphine; Favel, Anne; Grisel, Sacha; Henrissat, Bernard; Herpoël-Gimbert, Isabelle; Ruiz-Dueñas, Francisco J.; Chevret, Didier; Hainaut, Matthieu; Lin, Junyan; Wang, Mei; Pangilinan, Jasmyn; Lipzen, Anna; Lesage-Meessen, Laurence; Navarro, David; Riley, Robert; Grigoriev, Igor V. more »

Plant biomass conversion for green chemistry and bio-energy is a current challenge for a modern sustainable bioeconomy. The complex polyaromatic lignin polymers in raw biomass feedstocks (i.e., agriculture and forestry by-products) are major obstacles for biomass conversions. White-rot fungi are wood decayers able to degrade all polymers from lignocellulosic biomass including cellulose, hemicelluloses, and lignin. The white-rot fungus Polyporus brumalis efficiently breaks down lignin and is regarded as having a high potential for the initial treatment of plant biomass in its conversion to bio-energy. Here, we describe the extraordinary ability of P. brumalis for lignin degradation using its enzymatic arsenal to break down wheat straw, a lignocellulosic substrate that is considered as a biomass feedstock worldwide.We performed integrative multi-omics analyses by combining data from the fungal genome, transcriptomes, and secretomes. We found that the fungus possessed an unexpectedly large set of genes coding for Class II peroxidases involved in lignin degradation (19 genes) and GMC oxidoreductases/dehydrogenases involved in generating the hydrogen peroxide required for lignin peroxidase activity and promoting redox cycling of the fungal enzymes involved in oxidative cleavage of lignocellulose polymers (36 genes). The examination of interrelated multi-omics patterns revealed that eleven Class II Peroxidases were secreted by the fungus during fermentation and eight of them where tightly co-regulated with redox cycling enzymatic partners.As a peculiar feature of P. brumalis, we observed gene family extension, up-regulation and secretion of an abundant set of versatile peroxidases and manganese peroxidases, compared with other Polyporales species. The orchestrated secretion of an abundant set of these delignifying enzymes and redox cycling enzymatic partners could contribute to the delignification capabilities of the fungus. Our findings highlight the diversity of wood decay mechanisms present in Polyporales and the potentiality of further exploring this taxonomic order for enzymatic functions of biotechnological interest.

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Research Organization:: Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States). National Energy Research Scientific Computing Center (NERSC); Univ. of California, Oakland, CA (United States)

Sponsoring Organization:: USDOE Office of Science (SC); French National Agency for Research; Spanish Ministry of Economy, Industry and Competitiveness

Grant/Contract Number:: AC02-05CH11231; BIO2017-86559-R

OSTI ID:: 1618746

Alternate ID(s):: OSTI ID: 1544020; OSTI ID: 1619092

Journal Information:: Biotechnology for Biofuels, Journal Name: Biotechnology for Biofuels Vol. 11 Journal Issue: 1; ISSN 1754-6834

Publisher:: BioMed CentralCopyright Statement

Country of Publication:: Netherlands

Language:: English

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Cited by: 27 works

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References (66)

Systematic Identification and Evolutionary Analysis of Catalytically Versatile Cytochrome P450 Monooxygenase Families Enriched in Model Basidiomycete Fungi Syed, Khajamohiddin; Shale, Karabo; Pagadala, Nataraj Sekhar PLoS ONE, Vol. 9, Issue 1 https://doi.org/10.1371/journal.pone.0086683	journal	January 2014
Time-scale dynamics of proteome and transcriptome of the white-rot fungus Phlebia radiata: growth on spruce wood and decay effect on lignocellulose Kuuskeri, Jaana; Häkkinen, Mari; Laine, Pia Biotechnology for Biofuels, Vol. 9, Issue 1 https://doi.org/10.1186/s13068-016-0608-9	journal	September 2016
Genomewide analysis of polysaccharides degrading enzymes in 11 white- and brown-rot Polyporales provides insight into mechanisms of wood decay Hori, Chiaki; Gaskell, Jill; Igarashi, Kiyohiko Mycologia, Vol. 105, Issue 6 https://doi.org/10.3852/13-072	journal	November 2013
Multiplicity of enzymatic functions in the CAZy AA3 family Sützl, Leander; Laurent, Christophe V. F. P.; Abrera, Annabelle T. Applied Microbiology and Biotechnology, Vol. 102, Issue 6 https://doi.org/10.1007/s00253-018-8784-0	journal	February 2018
A General Empirical Model of Protein Evolution Derived from Multiple Protein Families Using a Maximum-Likelihood Approach Whelan, Simon; Goldman, Nick Molecular Biology and Evolution, Vol. 18, Issue 5 https://doi.org/10.1093/oxfordjournals.molbev.a003851	journal	May 2001
New Algorithms and Methods to Estimate Maximum-Likelihood Phylogenies: Assessing the Performance of PhyML 3.0 Guindon, Stéphane; Dufayard, Jean-François; Lefort, Vincent Systematic Biology, Vol. 59, Issue 3 https://doi.org/10.1093/sysbio/syq010	journal	March 2010
Cytochrome P450 enzymes in the fungal kingdom Črešnar, B.; Petrič, Š. Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics, Vol. 1814, Issue 1 https://doi.org/10.1016/j.bbapap.2010.06.020	journal	January 2011
Comparative and transcriptional analysis of the predicted secretome in the lignocellulose-degrading basidiomycete fungus Pleurotus ostreatus : Functional study of Alfaro, Manuel; Castanera, Raúl; Lavín, José L. Environmental Microbiology, Vol. 18, Issue 12 https://doi.org/10.1111/1462-2920.13360	journal	June 2016
Fast solubilization of recalcitrant cellulosic biomass by the basidiomycete fungus Laetisaria arvalisinvolves successive secretion of oxidative and hydrolytic enzymes Navarro, David; Rosso, Marie-Noëlle; Haon, Mireille Biotechnology for Biofuels, Vol. 7, Issue 1 https://doi.org/10.1186/s13068-014-0143-5	journal	October 2014
A PCR-based method to quantify fungal growth during pretreatment of lignocellulosic biomass Simeng, Zhou; Sacha, Grisel; Isabelle, Herpoël-Gimbert Journal of Microbiological Methods, Vol. 115 https://doi.org/10.1016/j.mimet.2015.05.024	journal	August 2015
Variation in the chemical composition of wheat straw: the role of tissue ratio and composition Collins, Samuel RA; Wellner, Nikolaus; Martinez Bordonado, Isabel Biotechnology for Biofuels, Vol. 7, Issue 1 https://doi.org/10.1186/s13068-014-0121-y	journal	August 2014
A novel glucose dehydrogenase from the white-rot fungus Pycnoporus cinnabarinus: production in Aspergillus niger and physicochemical characterization of the recombinant enzyme Piumi, François; Levasseur, Anthony; Navarro, David Applied Microbiology and Biotechnology, Vol. 98, Issue 24 https://doi.org/10.1007/s00253-014-5891-4	journal	June 2014
Screening of heterozygous DNA markers in shiitake (Lentinula edodes) using de-dikaryotization via preparation of protoplasts and isolation of four meiotic monokaryons from one basidium Miyazaki, Kazuhiro; Maeda, Hajime; Sunagawa, Masahide Journal of Wood Science, Vol. 46, Issue 5 https://doi.org/10.1007/BF00776403	journal	October 2000
High-quality draft assemblies of mammalian genomes from massively parallel sequence data Gnerre, S.; MacCallum, I.; Przybylski, D. Proceedings of the National Academy of Sciences, Vol. 108, Issue 4 https://doi.org/10.1073/pnas.1017351108	journal	December 2010
Single-domain flavoenzymes trigger lytic polysaccharide monooxygenases for oxidative degradation of cellulose Garajova, Sona; Mathieu, Yann; Beccia, Maria Rosa Scientific Reports, Vol. 6, Issue 1 https://doi.org/10.1038/srep28276	journal	June 2016
An alcohol oxidase of Phanerochaete chrysosporium with a distinct glycerol oxidase activity Linke, Diana; Lehnert, Nicole; Nimtz, Manfred Enzyme and Microbial Technology, Vol. 61-62 https://doi.org/10.1016/j.enzmictec.2014.04.001	journal	July 2014
Gapped BLAST and PSI-BLAST: a new generation of protein database search programs Altschul, Stephen F.; Madden, Thomas L.; Schäffer, Alejandro A. Nucleic Acids Research, Vol. 25, Issue 17, p. 3389-3402 https://doi.org/10.1093/nar/25.17.3389	journal	September 1997
Solid-state anaerobic digestion of fungal pretreated Miscanthus sinensis harvested in two different seasons Vasco-Correa, Juliana; Li, Yebo Bioresource Technology, Vol. 185 https://doi.org/10.1016/j.biortech.2015.02.099	journal	June 2015
Substrate oxidation sites in versatile peroxidase and other basidiomycete peroxidases Ruiz-Duenas, F. J.; Morales, M.; Garcia, E. Journal of Experimental Botany, Vol. 60, Issue 2 https://doi.org/10.1093/jxb/ern261	journal	January 2009
Phylogenetic and phylogenomic overview of the Polyporales Binder, Manfred; Justo, Alfredo; Riley, Robert Mycologia, Vol. 105, Issue 6 https://doi.org/10.3852/13-003	journal	November 2013
Improvement of Phylogenies after Removing Divergent and Ambiguously Aligned Blocks from Protein Sequence Alignments Talavera, Gerard; Castresana, Jose Systematic Biology, Vol. 56, Issue 4 https://doi.org/10.1080/10635150701472164	journal	August 2007
Activities of Secreted Aryl Alcohol Quinone Oxidoreductases from Pycnoporus cinnabarinus Provide Insights into Fungal Degradation of Plant Biomass Mathieu, Yann; Piumi, Francois; Valli, Richard Applied and Environmental Microbiology, Vol. 82, Issue 8 https://doi.org/10.1128/AEM.03761-15	journal	February 2016
Temporal Alterations in the Secretome of the Selective Ligninolytic Fungus Ceriporiopsis subvermispora during Growth on Aspen Wood Reveal This Organism's Strategy for Degrading Lignocellulose Hori, Chiaki; Gaskell, Jill; Igarashi, Kiyohiko Applied and Environmental Microbiology, Vol. 80, Issue 7 https://doi.org/10.1128/AEM.03652-13	journal	January 2014
Fungal Communities in Ancient Peatlands at Sanjiang Plain, China Zhou, Xue; Zhang, Zhenqing; Tian, Lei Fungal Genomics & Biology, Vol. 07, Issue 01 https://doi.org/10.4172/2165-8056.1000148	journal	January 2017
Highly Efficient Production of Laccase by the Basidiomycete Pycnoporus cinnabarinus Alves, Alexandra M. C. R.; Record, Eric; Lomascolo, Anne Applied and Environmental Microbiology, Vol. 70, Issue 11, p. 6379-6384 https://doi.org/10.1128/AEM.70.11.6379-6384.2004	journal	November 2004
Extracellular electron transfer systems fuel cellulose oxidative degradation Kracher, D.; Scheiblbrandner, S.; Felice, A. K. G. Science, Vol. 352, Issue 6289 https://doi.org/10.1126/science.aaf3165	journal	April 2016
Fungal pretreatment of lignocellulosic biomass Wan, Caixia; Li, Yebo Biotechnology Advances, Vol. 30, Issue 6 https://doi.org/10.1016/j.biotechadv.2012.03.003	journal	November 2012
Visual Comparative Omics of Fungi for Plant Biomass Deconstruction Miyauchi, Shingo; Navarro, David; Grigoriev, Igor V. Frontiers in Microbiology, Vol. 7 https://doi.org/10.3389/fmicb.2016.01335	journal	August 2016
OrthoMCL: Identification of Ortholog Groups for Eukaryotic Genomes Li, L. Genome Research, Vol. 13, Issue 9 https://doi.org/10.1101/gr.1224503	journal	September 2003
A Combined Transmembrane Topology and Signal Peptide Prediction Method Käll, Lukas; Krogh, Anders; Sonnhammer, Erik L. L. Journal of Molecular Biology, Vol. 338, Issue 5 https://doi.org/10.1016/j.jmb.2004.03.016	journal	May 2004
Fungal pretreatment of switchgrass for improved saccharification and simultaneous enzyme production Liu, Jiayang; Wang, Ming Li; Tonnis, Brandon Bioresource Technology, Vol. 135 https://doi.org/10.1016/j.biortech.2012.10.095	journal	May 2013
Comparative analysis of the secretomes of Schizophyllum commune and other wood-decay basidiomycetes during solid-state fermentation reveals its unique lignocellulose-degrading enzyme system Zhu, Ning; Liu, Jiawen; Yang, Jinshui Biotechnology for Biofuels, Vol. 9, Issue 1 https://doi.org/10.1186/s13068-016-0461-x	journal	February 2016
Lignin-degrading peroxidases in Polyporales: an evolutionary survey based on 10 sequenced genomes Ruiz-Dueñas, Francisco J.; Lundell, Taina; Floudas, Dimitrios Mycologia, Vol. 105, Issue 6 https://doi.org/10.3852/13-059	journal	November 2013
Interactive tree of life (iTOL) v3: an online tool for the display and annotation of phylogenetic and other trees Letunic, Ivica; Bork, Peer Nucleic Acids Research, Vol. 44, Issue W1 https://doi.org/10.1093/nar/gkw290	journal	April 2016
RAxML version 8: a tool for phylogenetic analysis and post-analysis of large phylogenies Stamatakis, Alexandros Bioinformatics, Vol. 30, Issue 9 https://doi.org/10.1093/bioinformatics/btu033	journal	January 2014
MAFFT: a novel method for rapid multiple sequence alignment based on fast Fourier transform Katoh, K. Nucleic Acids Research, Vol. 30, Issue 14 https://doi.org/10.1093/nar/gkf436	journal	July 2002
Biological wheat straw valorization: Multicriteria optimization of Polyporus brumalis pretreatment in packed bed bioreactor Zhou, Simeng; Herpoël-Gimbert, Isabelle; Grisel, Sacha MicrobiologyOpen, Vol. 7, Issue 1 https://doi.org/10.1002/mbo3.530	journal	October 2017
The Paleozoic Origin of Enzymatic Lignin Decomposition Reconstructed from 31 Fungal Genomes Floudas, Dimitrios; Binder, Manfred; Riley, Robert Science, Vol. 336, Issue 6089 https://doi.org/10.1126/science.1221748	journal	June 2012
A survey of genes encoding H2O2-producing GMC oxidoreductases in 10 Polyporales genomes Ferreira, P.; Carro, J.; Serrano, A. Mycologia, Vol. 107, Issue 6 https://doi.org/10.3852/15-027	journal	August 2015
Insight into trade-off between wood decay and parasitism from the genome of a fungal forest pathogen Olson, Åke; Aerts, Andrea; Asiegbu, Fred New Phytologist, Vol. 194, Issue 4 https://doi.org/10.1111/j.1469-8137.2012.04128.x	journal	March 2012
Effect of microbial pretreatment on enzymatic hydrolysis and fermentation of cotton stalks for ethanol production Shi, Jian; Sharma-Shivappa, Ratna R.; Chinn, Mari Biomass and Bioenergy, Vol. 33, Issue 1 https://doi.org/10.1016/j.biombioe.2008.04.016	journal	January 2009
Use of Dinitrosalicylic Acid Reagent for Determination of Reducing Sugar Miller, G. L. Analytical Chemistry, Vol. 31, Issue 3 https://doi.org/10.1021/ac60147a030	journal	March 1959
The carbohydrate-active enzymes database (CAZy) in 2013 Lombard, Vincent; Golaconda Ramulu, Hemalatha; Drula, Elodie Nucleic Acids Research, Vol. 42, Issue D1 https://doi.org/10.1093/nar/gkt1178	journal	November 2013
M-Coffee: combining multiple sequence alignment methods with T-Coffee Wallace, I. M. Nucleic Acids Research, Vol. 34, Issue 6 https://doi.org/10.1093/nar/gkl091	journal	March 2006
Fungal pretreatment of cornstalk with Phanerochaete chrysosporium for enhancing enzymatic saccharification and hydrogen production Zhao, Lei; Cao, Guang-Li; Wang, Ai-Jie Bioresource Technology, Vol. 114 https://doi.org/10.1016/j.biortech.2012.03.076	journal	June 2012
Genomics of wood-degrading fungi Ohm, Robin A.; Riley, Robert; Salamov, Asaf Fungal Genetics and Biology, Vol. 72 https://doi.org/10.1016/j.fgb.2014.05.001	journal	November 2014
Cytochrome P450 Monooxygenase CYP53 Family in Fungi: Comparative Structural and Evolutionary Analysis and Its Role as a Common Alternative Anti-Fungal Drug Target Jawallapersand, Poojah; Mashele, Samson Sitheni; Kovačič, Lidija PLoS ONE, Vol. 9, Issue 9 https://doi.org/10.1371/journal.pone.0107209	journal	September 2014
Estimating Gene Gain and Loss Rates in the Presence of Error in Genome Assembly and Annotation Using CAFE 3 Han, Mira V.; Thomas, Gregg W. C.; Lugo-Martinez, Jose Molecular Biology and Evolution, Vol. 30, Issue 8 https://doi.org/10.1093/molbev/mst100	journal	May 2013
Genome-Wide Annotation and Comparative Analysis of Cytochrome P450 Monooxygenases in Basidiomycete Biotrophic Plant Pathogens Qhanya, Lehlohonolo Benedict; Matowane, Godfrey; Chen, Wanping PLOS ONE, Vol. 10, Issue 11 https://doi.org/10.1371/journal.pone.0142100	journal	November 2015
Comparative genomics of Ceriporiopsis subvermispora and Phanerochaete chrysosporium provide insight into selective ligninolysis Fernandez-Fueyo, E.; Ruiz-Duenas, F. J.; Ferreira, P. Proceedings of the National Academy of Sciences, Vol. 109, Issue 14, p. 5458-5463 https://doi.org/10.1073/pnas.1119912109	journal	March 2012
Predicting Subcellular Localization of Proteins Based on their N-terminal Amino Acid Sequence Emanuelsson, Olof; Nielsen, Henrik; Brunak, Søren Journal of Molecular Biology, Vol. 300, Issue 4 https://doi.org/10.1006/jmbi.2000.3903	journal	July 2000
The integrative omics of white-rot fungus Pycnoporus coccineus reveals co-regulated CAZymes for orchestrated lignocellulose breakdown Miyauchi, Shingo; Navarro, David; Grisel, Sacha PLOS ONE, Vol. 12, Issue 4 https://doi.org/10.1371/journal.pone.0175528	journal	April 2017
Protein structure homology modeling using SWISS-MODEL workspace Bordoli, Lorenza; Kiefer, Florian; Arnold, Konstantin Nature Protocols, Vol. 4, Issue 1 https://doi.org/10.1038/nprot.2008.197	journal	December 2008
Systematic and searchable classification of cytochrome P450 proteins encoded by fungal and oomycete genomes Moktali, Venkatesh; Park, Jongsun; Fedorova-Abrams, Natalie D. BMC Genomics, Vol. 13, Issue 1 https://doi.org/10.1186/1471-2164-13-525	journal	January 2012
Enhanced degradation of softwood versus hardwood by the white-rot fungus Pycnoporus coccineus Couturier, Marie; Navarro, David; Chevret, Didier Biotechnology for Biofuels, Vol. 8, Issue 1 https://doi.org/10.1186/s13068-015-0407-8	journal	December 2015
Solid-state fermentation in multi-well plates to assess pretreatment efficiency of rot fungi on lignocellulose biomass: Multi-well plate SSF to assess fungal pretreatment Zhou, Simeng; Raouche, Sana; Grisel, Sacha Microbial Biotechnology, Vol. 8, Issue 6 https://doi.org/10.1111/1751-7915.12307	journal	August 2015
The COG database: an updated version includes eukaryotes Tatusov, Roman L.; Fedorova, Natalie D.; Jackson, John D. BMC Bioinformatics, Vol. 4, Article No. 41 https://doi.org/10.1186/1471-2105-4-41	journal	January 2003
HMMER web server: interactive sequence similarity searching Finn, R. D.; Clements, J.; Eddy, S. R. Nucleic Acids Research, Vol. 39, Issue suppl https://doi.org/10.1093/nar/gkr367	journal	May 2011
Independent evolution of four heme peroxidase superfamilies Zámocký, Marcel; Hofbauer, Stefan; Schaffner, Irene Archives of Biochemistry and Biophysics, Vol. 574 https://doi.org/10.1016/j.abb.2014.12.025	journal	May 2015
Rnnotator: an automated de novo transcriptome assembly pipeline from stranded RNA-Seq reads Martin, Jeffrey; Bruno, Vincent M.; Fang, Zhide BMC Genomics, Vol. 11, Issue 1 https://doi.org/10.1186/1471-2164-11-663	journal	January 2010
MycoCosm portal: gearing up for 1000 fungal genomes Grigoriev, Igor V.; Nikitin, Roman; Haridas, Sajeet Nucleic Acids Research, Vol. 42, Issue D1 https://doi.org/10.1093/nar/gkt1183	journal	December 2013
The genome of the white-rot fungus Pycnoporus cinnabarinus: a basidiomycete model with a versatile arsenal for lignocellulosic biomass breakdown Levasseur, Anthony; Lomascolo, Anne; Chabrol, Olivier BMC Genomics, Vol. 15, Issue 1 https://doi.org/10.1186/1471-2164-15-486	journal	January 2014
Extensive sampling of basidiomycete genomes demonstrates inadequacy of the white-rot/brown-rot paradigm for wood decay fungi Riley, Robert; Salamov, Asaf A.; Brown, Daren W. Proceedings of the National Academy of Sciences, Vol. 111, Issue 27 https://doi.org/10.1073/pnas.1400592111	journal	June 2014
Moderated estimation of fold change and dispersion for RNA-seq data with DESeq2 Love, Michael I.; Huber, Wolfgang; Anders, Simon Genome Biology, Vol. 15, Issue 12 https://doi.org/10.1186/s13059-014-0550-8	journal	December 2014
Expansion of the enzymatic repertoire of the CAZy database to integrate auxiliary redox enzymes Levasseur, Anthony; Drula, Elodie; Lombard, Vincent Biotechnology for Biofuels, Vol. 6, Issue 1, Article No. 41 https://doi.org/10.1186/1754-6834-6-41	journal	January 2013
Transcriptional and Enzymatic Profiling of Pleurotus ostreatus Laccase Genes in Submerged and Solid-State Fermentation Cultures Castanera, Raúl; Pérez, Gúmer; Omarini, Alejandra Applied and Environmental Microbiology, Vol. 78, Issue 11 https://doi.org/10.1128/AEM.07880-11	journal	March 2012

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Title: Integrative visual omics of the white-rot fungus Polyporus brumalis exposes the biotechnological potential of its oxidative enzymes for delignifying raw plant biomass

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