Overexpression of a Domain of Unknown Function 266-containing protein results in high cellulose content, reduced recalcitrance, and enhanced plant growth in the bioenergy crop Populus

Yang, Yongil; Yoo, Chang Geun; Guo, Hao-Bo; Rottmann, William; Winkeler, Kimberly A.; Collins, Cassandra M.; Gunter, Lee E.; Jawdy, Sara S.; Yang, Xiaohan; Guo, Hong; Pu, Yunqiao; Ragauskas, Arthur J.; Tuskan, Gerald A.; Chen, Jin-Gui

doi:10.1186/s13068-017-0760-x

Title: Overexpression of a Domain of Unknown Function 266-containing protein results in high cellulose content, reduced recalcitrance, and enhanced plant growth in the bioenergy crop Populus

Journal Article · Thu Mar 23 00:00:00 EDT 2017 · Biotechnology for Biofuels

DOI:https://doi.org/10.1186/s13068-017-0760-x· OSTI ID:1618674

Yang, Yongil; Yoo, Chang Geun; Guo, Hao-Bo; Rottmann, William; Winkeler, Kimberly A.; Collins, Cassandra M.; Gunter, Lee E.; Jawdy, Sara S.; Yang, Xiaohan; Guo, Hong; Pu, Yunqiao; Ragauskas, Arthur J.; Tuskan, Gerald A.;

Domain of Unknown Function 266 (DUF266) is a plant-specific domain. DUF266-containing proteins (DUF266 proteins) have been categorized as ‘not classified glycosyltransferases (GTnc)’ due to amino acid similarity with GTs. However, little is known about the function of DUF266 proteins. As a result, Phylogenetic analysis revealed that DUF266 proteins are only present in the land plants including moss and lycophyte. We report the functional characterization of one member of DUF266 proteins in Populus, PdDUF266A. PdDUF266A was ubiquitously expressed with high abundance in the xylem. In Populus transgenic plants overexpressing PdDUF266A (OXPdDUF266A), the glucose and cellulose contents were significantly higher, while the lignin content was lower than that in the wild type. Degree of polymerization of cellulose in OXPdDUF266A transgenic plants was also higher, whereas cellulose crystallinity index remained unchanged. Gene expression analysis indicated that cellulose biosynthesis-related genes such as CESA and SUSY were upregulated in mature leaf and xylem of OXPdDUF266A transgenic plants. Moreover, PdDUF266A overexpression resulted in an increase of biomass production. Their glucose contents and biomass phenotypes were further validated via heterologous expression of PdDUF266A in Arabidopsis. Results from saccharification treatment demonstrated that the rate of sugar release was increased by approximately 38% in the OXPdDUF266A transgenic plants.

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Research Organization:: Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States). BioEnergy Science Center (BESC); Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States). Joint Institute for Biological Sciences (JIBS)

Sponsoring Organization:: USDOE Office of Science (SC)

Grant/Contract Number:: AC05-00OR22725

OSTI ID:: 1618674

Alternate ID(s):: OSTI ID: 1356900

Journal Information:: Biotechnology for Biofuels, Journal Name: Biotechnology for Biofuels Vol. 10 Journal Issue: 1; ISSN 1754-6834

Publisher:: Springer Science + Business MediaCopyright Statement

Country of Publication:: Netherlands

Language:: English

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Cited by: 16 works

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Related Subjects

09 BIOMASS FUELS
60 APPLIED LIFE SCIENCES
DUF266
recalcitrance
Populus
bioenergy
biofuel
biomass
cell wall
cellulose
sugar release

Overexpression of a Domain of Unknown Function 266-containing protein results in high cellulose content, reduced recalcitrance, and enhanced plant growth in the bioenergy crop Populus [Supplemental Data] Yoo, Chang; Guo, Hao-Bo; Rottmann, William https://doi.org/10.6084/m9.figshare.c.3725197.v1 The current record is supplemented by this collection	collection	March 2017
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MOESM8 of Overexpression of a Domain of Unknown Function 266-containing protein results in high cellulose content, reduced recalcitrance, and enhanced plant growth in the bioenergy crop Populus Yang, Yongil; Yoo, Chang; Guo, Hao-Bo https://doi.org/10.6084/m9.figshare.c.3725197_d8.v1 The current record is supplemented by this dataset	dataset	March 2017
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