A fixed-target platform for serial femtosecond crystallography in a hydrated environment

Shelby, M. L.; Gilbile, D.; Grant, T. D.; Seuring, C.; Segelke, B. W.; He, W.; Evans, A. C.; Pakendorf, T.; Fischer, P.; Hunter, M. S.; Batyuk, A.; Barthelmess, M.; Meents, A.; Coleman, M. A.; Kuhl, T. L.; Frank, M.

doi:10.1107/S2052252519014003

Title: A fixed-target platform for serial femtosecond crystallography in a hydrated environment

Journal Article · Wed Jan 01 00:00:00 EST 2020 · IUCrJ

DOI:https://doi.org/10.1107/S2052252519014003· OSTI ID:1617950

Shelby, M. L.; Gilbile, D.;

; Seuring, C.; Segelke, B. W.; He, W.; Evans, A. C.; Pakendorf, T.; Fischer, P.; Hunter, M. S.; Batyuk, A.; Barthelmess, M.;

; Coleman, M. A.; Kuhl, T. L.; Frank, M.

For serial femtosecond crystallography at X-ray free-electron lasers, which entails collection of single-pulse diffraction patterns from a constantly refreshed supply of microcrystalline sample, delivery of the sample into the X-ray beam path while maintaining low background remains a technical challenge for some experiments, especially where this methodology is applied to relatively low-ordered samples or those difficult to purify and crystallize in large quantities. This work demonstrates a scheme to encapsulate biological samples using polymer thin films and graphene to maintain sample hydration in vacuum conditions. The encapsulated sample is delivered into the X-ray beam on fixed targets for rapid scanning using the Roadrunner fixed-target system towards a long-term goal of low-background measurements on weakly diffracting samples. As a proof of principle, we used microcrystals of the 24 kDa rapid encystment protein (REP24) to provide a benchmark for polymer/graphene sandwich performance. The REP24 microcrystal unit cell obtained from our sandwiched in-vacuum sample was consistent with previously established unit-cell parameters and with those measured by us without encapsulation in humidified helium, indicating that the platform is robust against evaporative losses. While significant scattering from water was observed because of the sample-deposition method, the polymer/graphene sandwich itself was shown to contribute minimally to background scattering.

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Research Organization:: SLAC National Accelerator Laboratory (SLAC), Menlo Park, CA (United States). Linac Coherent Light Source (LCLS); Lawrence Livermore National Laboratory (LLNL), Livermore, CA (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Basic Energy Sciences (BES); National Institutes of Health (NIH); USDOE National Nuclear Security Administration (NNSA)

Grant/Contract Number:: AC52-07NA27344; AC02-76SF00515; 1R01GM117342-01; STC-1231306

OSTI ID:: 1617950

Alternate ID(s):: OSTI ID: 1605168; OSTI ID: 1614970

Report Number(s):: LLNL-JRNL-773953; IUCRAJ; PII: S2052252519014003

Journal Information:: IUCrJ, Journal Name: IUCrJ Vol. 7 Journal Issue: 1; ISSN 2052-2525

Publisher:: International Union of Crystallography (IUCr)Copyright Statement

Country of Publication:: United Kingdom

Language:: English

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Cited by: 15 works

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Title: A fixed-target platform for serial femtosecond crystallography in a hydrated environment

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