The XFP (17-BM) beamline for X-ray footprinting at NSLS-II

Asuru, Awuri; Farquhar, Erik R.; Sullivan, Michael; Abel, Donald; Toomey, John; Chance, Mark R.; Bohon, Jen

doi:10.1107/S1600577519003576

Title: The XFP (17-BM) beamline for X-ray footprinting at NSLS-II

Journal Article · Tue Jun 04 00:00:00 EDT 2019 · Journal of Synchrotron Radiation (Online)

DOI:https://doi.org/10.1107/S1600577519003576· OSTI ID:1617933

Asuru, Awuri;

; Sullivan, Michael; Abel, Donald; Toomey, John;

;

Hydroxyl-radical mediated synchrotron X-ray footprinting (XF) is a powerful solution-state technique in structural biology for the study of macromolecular structure and dynamics of proteins and nucleic acids, with several synchrotron resources available to serve the XF community worldwide. The XFP (Biological X-ray Footprinting) beamline at the NSLS-II was constructed on a three-pole wiggler source at 17-BM to serve as the premier beamline for performing this technique, providing an unparalleled combination of high flux density broadband beam, flexibility in beam morphology, and sample handling capabilities specifically designed for XF experiments. The details of beamline design, beam measurements, and science commissioning results for a standard protein using the two distinct XFP endstations are presented here. XFP took first light in 2016 and is now available for general user operations through peer-reviewed proposals. Currently, beam sizes from 450 µm × 120 µm to 2.7 mm × 2.7 mm (FWHM) are available, with a flux of 1.6 × 1016 photons s-1 (measured at 325 mA ring current) in a broadband (~5–16 keV) beam. This flux is expected to rise to 2.5 × 1016 photons s-1 at the full NSLS-II design current of 500 mA, providing an incident power density of >500 W mm-2 at full focus.

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Research Organization:: Sydor Instruments, LLC, Fairport, NY (United States); Brookhaven National Laboratory (BNL), Upton, NY (United States)

Sponsoring Organization:: USDOE Office of Science (SC); National Science Foundation (NSF)

Grant/Contract Number:: SC0012704; SC0007482; 1228549; 1255340

OSTI ID:: 1617933

Alternate ID(s):: OSTI ID: 1610957; OSTI ID: 1688802; OSTI ID: 1808964

Report Number(s):: BNL-220134-2020-JACI; BNL-221859-2021-JACI; JSYRES; PII: S1600577519003576

Journal Information:: Journal of Synchrotron Radiation (Online), Journal Name: Journal of Synchrotron Radiation (Online) Vol. 26 Journal Issue: 4; ISSN 1600-5775

Publisher:: International Union of Crystallography (IUCr)Copyright Statement

Country of Publication:: Denmark

Language:: English

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Cited by: 13 works

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