Micro-XRF mapping and quantitative assessment of Cd in rice (Oryza sativa L.) roots

Tefera, Wolde; Liu, Ting; Lu, Lingli; Ge, Jun; Webb, Samuel M.; Seifu, Weldemariam; Tian, Shengke

doi:10.1016/j.ecoenv.2020.110245

Micro-XRF mapping and quantitative assessment of Cd in rice (Oryza sativa L.) roots

Journal Article · Fri Feb 21 00:00:00 EST 2020 · Ecotoxicology and Environmental Safety

DOI:https://doi.org/10.1016/j.ecoenv.2020.110245· OSTI ID:1616720

Tefera, Wolde ^[1]; Liu, Ting ^[2]; Lu, Lingli ^[2]; Ge, Jun ^[2]; Webb, Samuel M. ^[3]; Seifu, Weldemariam ^[1]; Tian, Shengke ^[2]

Ministry of Education, Hangzhou (China); Salale Univ., Fitche (Ethiopia)
Ministry of Education, Hangzhou (China)
SLAC National Accelerator Lab., Menlo Park, CA (United States)

Understanding Cd uptake and distribution in rice roots is important for breeding varieties that do not accumulate Cd in the grain to any large extent. Here, we examined the physiological and molecular factors responsible for Cd uptake and transport differences between two japonica rice cultivars prescreened as high (zhefu7) or low (Xiangzaoxian45) accumulators of Cd in the grain. No significant differences in Cd uptake between the two cultivars were observed; however, Xiangzaoxian45 retained most of the absorbed Cd in the roots, whereas zhefu7 showed higher transport of Cd from the root to the shoot, regardless of the duration of exposure to Cd. The inability to sequester Cd into root vacuoles caused high accumulation of Cd in the grain in zhefu7, whereas inefficient transport of Cd from roots to shoots in Xiangzaoxian45 caused low accumulation of Cd in the grain. Cd sequestration in the roots and transport from the root to the shoot were greatly influenced by the expression patterns of transport-related genes OsHMA3 and OsHMA2, respectively. Further, micro-X-ray fluorescence spectroscopy mapping confirmed that more Cd was sequestered in the roots of Xiangzaoxian45 than in those of zhefu7, with a significant amount of Cd localized in the root hairs, as well as in the meristematic and elongation zones, and dermal and stele tissues. Finally, we propose that effective Cd sequestration in root vacuoles was the major determinant of divergent Cd-accumulation patterns in the two rice cultivars under study.

View Accepted Manuscript (DOE)

Research Organization:: SLAC National Accelerator Laboratory (SLAC), Menlo Park, CA (United States)

Sponsoring Organization:: National Key Research and Development Plan; National Natural Science Foundation of China (NNSFC); USDOE

Grant/Contract Number:: AC02-76SF00515

OSTI ID:: 1616720

Journal Information:: Ecotoxicology and Environmental Safety, Journal Name: Ecotoxicology and Environmental Safety Journal Issue: C Vol. 193; ISSN 0147-6513

Publisher:: ElsevierCopyright Statement

Country of Publication:: United States

Language:: English

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59 BASIC BIOLOGICAL SCIENCES
Cd sequestration
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micro-x-ray fluorescence
rice
root-to-shoot transport

Micro-XRF mapping and quantitative assessment of Cd in rice (Oryza sativa L.) roots

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