Sequencing wild and cultivated cassava and related species reveals extensive interspecific hybridization and genetic diversity

Bredeson, Jessen V.; Lyons, Jessica B.; Prochnik, Simon E.; Wu, G. Albert; Ha, Cindy M.; Edsinger-Gonzales, Eric; Grimwood, Jane; Schmutz, Jeremy; Rabbi, Ismail Y.; Egesi, Chiedozie; Nauluvula, Poasa; Lebot, Vincent; Ndunguru, Joseph; Mkamilo, Geoffrey; Bart, Rebecca S.; Setter, Tim L.; Gleadow, Roslyn M.; Kulakow, Peter; Ferguson, Morag E.; Rounsley, Steve; Rokhsar, Daniel S.

doi:10.1038/nbt.3535

Sequencing wild and cultivated cassava and related species reveals extensive interspecific hybridization and genetic diversity

Journal Article · Mon Apr 18 00:00:00 EDT 2016 · Nature Biotechnology

DOI:https://doi.org/10.1038/nbt.3535· OSTI ID:1616047

Bredeson, Jessen V. ^[1]; ^[1]; Prochnik, Simon E. ^[2]; Wu, G. Albert ^[2]; Ha, Cindy M. ^[3]; Edsinger-Gonzales, Eric ^[3]; Grimwood, Jane ^[4]; Schmutz, Jeremy ^[4]; Rabbi, Ismail Y. ^[5]; Egesi, Chiedozie ^[6]; Nauluvula, Poasa ^[7]; Lebot, Vincent ^[8]; Ndunguru, Joseph ^[9]; Mkamilo, Geoffrey ^[10]; Bart, Rebecca S. ^[11]; Setter, Tim L. ^[12]; ^[13]; Kulakow, Peter ^[5]; Ferguson, Morag E. ^[14]; ^[15] more »

Univ. of California, Berkeley, CA (United States)
USDOE Joint Genome Institute (JGI), Walnut Creek, CA (United States)
Univ. of California, Berkeley, CA (United States); Univ. of Colorado, Denver, CO (United States)
USDOE Joint Genome Institute (JGI), Walnut Creek, CA (United States); HudsonAlpha Institute for Biotechnology, Huntsville, AL (United States)
International Institute of Tropical Agriculture (IITA), Ibadan (Nigeria)
National Root Crops Research Institute (NRCRI), Umudike (Nigeria)
Ministry of Primary Industries, Koronivia Research Station (Fiji)
Centre de coopération Internationale en Recherche Agronomique pour le Développement (CIRAD), Port-Vila (Vanuatu)
Mikocheni Agricultural Research Institute (MARI), Dar es Salaam (Tanzania)
Naliendele Agricultural Research Institute (NARI), Mtwara (Tanzania)
Donald Danforth Plant Science Center, St. Louis, MO (United States)
Cornell Univ., Ithaca, NY (United States)
Monash Univ., Melbourne, VIC (Australia)
International Institute of Tropical Agriculture (IITA), Nairobi (Kenya)
Dow AgroSciences, Indianapolis, IN (United States)
Univ. of California, Berkeley, CA (United States); USDOE Joint Genome Institute (JGI), Walnut Creek, CA (United States); Okinawa Institute of Science and Technology Graduate University, Onna (Japan)

Cassava (Manihot esculenta) provides calories and nutrition for more than half a billion people. It was domesticated by native Amazonian peoples through cultivation of the wild progenitor M. esculenta ssp. flabellifolia and is now grown in tropical regions worldwide. Here we provide a high-quality genome assembly for cassava with improved contiguity, linkage, and completeness; almost 97% of genes are anchored to chromosomes. We find that paleotetraploidy in cassava is shared with the related rubber tree Hevea, providing a resource for comparative studies. We also sequence a global collection of 58 Manihot accessions, including cultivated and wild cassava accessions and related species such as Ceará or India rubber (M. glaziovii), and genotype 268 African cassava varieties. We find widespread interspecific admixture, and detect the genetic signature of past cassava breeding programs. As a clonally propagated crop, cassava is especially vulnerable to pathogens and abiotic stresses. This genomic resource will inform future genome-enabled breeding efforts to improve this staple crop.

View Accepted Manuscript (DOE)

Research Organization:: Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)

Sponsoring Organization:: Bill and Melinda Gates Foundation (BMGF); National Institutes of Health (NIH); USDOE Office of Science (SC), Biological and Environmental Research (BER)

Grant/Contract Number:: AC02-05CH11231

OSTI ID:: 1616047

Journal Information:: Nature Biotechnology, Journal Name: Nature Biotechnology Journal Issue: 5 Vol. 34; ISSN 1087-0156

Publisher:: Springer NatureCopyright Statement

Country of Publication:: United States

Language:: English

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Additional file 10 of Resequencing of 388 cassava accessions identifies valuable loci and selection for variation in heterozygosity Hu, Wei; Ji, Changmian; Liang, Zhe figshare https://doi.org/10.6084/m9.figshare.17030756	dataset	January 2021
Additional file 11 of Resequencing of 388 cassava accessions identifies valuable loci and selection for variation in heterozygosity Hu, Wei; Ji, Changmian; Liang, Zhe figshare https://doi.org/10.6084/m9.figshare.17030759.v1	dataset	January 2021
Additional file 12 of Resequencing of 388 cassava accessions identifies valuable loci and selection for variation in heterozygosity Hu, Wei; Ji, Changmian; Liang, Zhe figshare https://doi.org/10.6084/m9.figshare.17030762.v1	dataset	January 2021
Additional file 13 of Resequencing of 388 cassava accessions identifies valuable loci and selection for variation in heterozygosity Hu, Wei; Ji, Changmian; Liang, Zhe figshare https://doi.org/10.6084/m9.figshare.17030765	dataset	January 2021
Additional file 14 of Resequencing of 388 cassava accessions identifies valuable loci and selection for variation in heterozygosity Hu, Wei; Ji, Changmian; Liang, Zhe figshare https://doi.org/10.6084/m9.figshare.17030767	dataset	January 2021
Additional file 15 of Resequencing of 388 cassava accessions identifies valuable loci and selection for variation in heterozygosity Hu, Wei; Ji, Changmian; Liang, Zhe figshare https://doi.org/10.6084/m9.figshare.17030770.v1	dataset	January 2021
Additional file 16 of Resequencing of 388 cassava accessions identifies valuable loci and selection for variation in heterozygosity Hu, Wei; Ji, Changmian; Liang, Zhe figshare https://doi.org/10.6084/m9.figshare.17030773.v1	dataset	January 2021
Additional file 17 of Resequencing of 388 cassava accessions identifies valuable loci and selection for variation in heterozygosity Hu, Wei; Ji, Changmian; Liang, Zhe figshare https://doi.org/10.6084/m9.figshare.17030776.v1	dataset	January 2021
Additional file 18 of Resequencing of 388 cassava accessions identifies valuable loci and selection for variation in heterozygosity Hu, Wei; Ji, Changmian; Liang, Zhe figshare https://doi.org/10.6084/m9.figshare.17030779.v1	dataset	January 2021
Additional file 19 of Resequencing of 388 cassava accessions identifies valuable loci and selection for variation in heterozygosity Hu, Wei; Ji, Changmian; Liang, Zhe figshare https://doi.org/10.6084/m9.figshare.17030782.v1	dataset	January 2021
Additional file 1 of Resequencing of 388 cassava accessions identifies valuable loci and selection for variation in heterozygosity Hu, Wei; Ji, Changmian; Liang, Zhe figshare https://doi.org/10.6084/m9.figshare.17030785	dataset	January 2021
Additional file 20 of Resequencing of 388 cassava accessions identifies valuable loci and selection for variation in heterozygosity Hu, Wei; Ji, Changmian; Liang, Zhe figshare https://doi.org/10.6084/m9.figshare.17030791.v1	dataset	January 2021
Additional file 21 of Resequencing of 388 cassava accessions identifies valuable loci and selection for variation in heterozygosity Hu, Wei; Ji, Changmian; Liang, Zhe figshare https://doi.org/10.6084/m9.figshare.17030794.v1	dataset	January 2021
Additional file 2 of Resequencing of 388 cassava accessions identifies valuable loci and selection for variation in heterozygosity Hu, Wei; Ji, Changmian; Liang, Zhe figshare https://doi.org/10.6084/m9.figshare.17030803	dataset	January 2021
Additional file 3 of Resequencing of 388 cassava accessions identifies valuable loci and selection for variation in heterozygosity Hu, Wei; Ji, Changmian; Liang, Zhe figshare https://doi.org/10.6084/m9.figshare.17030806	dataset	January 2021
Additional file 4 of Resequencing of 388 cassava accessions identifies valuable loci and selection for variation in heterozygosity Hu, Wei; Ji, Changmian; Liang, Zhe figshare https://doi.org/10.6084/m9.figshare.17030809.v1	dataset	January 2021
Additional file 5 of Resequencing of 388 cassava accessions identifies valuable loci and selection for variation in heterozygosity Hu, Wei; Ji, Changmian; Liang, Zhe figshare https://doi.org/10.6084/m9.figshare.17030815	dataset	January 2021
Additional file 6 of Resequencing of 388 cassava accessions identifies valuable loci and selection for variation in heterozygosity Hu, Wei; Ji, Changmian; Liang, Zhe figshare https://doi.org/10.6084/m9.figshare.17030818	dataset	January 2021
Additional file 7 of Resequencing of 388 cassava accessions identifies valuable loci and selection for variation in heterozygosity Hu, Wei; Ji, Changmian; Liang, Zhe figshare https://doi.org/10.6084/m9.figshare.17030821.v1	dataset	January 2021
Additional file 8 of Resequencing of 388 cassava accessions identifies valuable loci and selection for variation in heterozygosity Hu, Wei; Ji, Changmian; Liang, Zhe figshare https://doi.org/10.6084/m9.figshare.17030824.v1	dataset	January 2021
Additional file 9 of Resequencing of 388 cassava accessions identifies valuable loci and selection for variation in heterozygosity Hu, Wei; Ji, Changmian; Liang, Zhe figshare https://doi.org/10.6084/m9.figshare.17030827.v1	dataset	January 2021
Additional file 3 of Genomic insights into lineage-specific evolution of the oleosin family in Euphorbiaceae Zou, Zhi; Zhao, Yongguo; Zhang, Li figshare https://doi.org/10.6084/m9.figshare.19311422.v1	dataset	January 2022
Additional file 4 of Genomic insights into lineage-specific evolution of the oleosin family in Euphorbiaceae Zou, Zhi; Zhao, Yongguo; Zhang, Li figshare https://doi.org/10.6084/m9.figshare.19311425	dataset	January 2022
Additional file 5 of Genomic insights into lineage-specific evolution of the oleosin family in Euphorbiaceae Zou, Zhi; Zhao, Yongguo; Zhang, Li figshare https://doi.org/10.6084/m9.figshare.19311428	dataset	January 2022
Additional file 7 of Genomic insights into lineage-specific evolution of the oleosin family in Euphorbiaceae Zou, Zhi; Zhao, Yongguo; Zhang, Li figshare https://doi.org/10.6084/m9.figshare.19311434	dataset	January 2022
Additional file 8 of Genomic insights into lineage-specific evolution of the oleosin family in Euphorbiaceae Zou, Zhi; Zhao, Yongguo; Zhang, Li figshare https://doi.org/10.6084/m9.figshare.19311437.v1	dataset	January 2022
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Identification of FT family genes that respond to photoperiod, temperature and genotype in relation to flowering in cassava (Manihot esculenta, Crantz) Adeyemo, Oluwabusayo Sarah; Hyde, Peter T.; Setter, Tim L. Plant Reproduction, Vol. 32, Issue 2 https://doi.org/10.1007/s00497-018-00354-5	journal	December 2018
Effect of temperature on development and reproduction of the carmine spider mite, Tetranychus cinnabarinus (Acari: Tetranychiae), fed on cassava leaves Zou, Zhiwen; Xi, Jianfei; Liu, Ge Experimental and Applied Acarology, Vol. 74, Issue 4 https://doi.org/10.1007/s10493-018-0241-3	journal	March 2018
Genomic Diversity of Three Brazilian Native Food Crops Based on Double-Digest Restriction Site-Associated DNA Sequencing Alves-Pereira, Alessandro; Novello, Mariana; Dequigiovanni, Gabriel Tropical Plant Biology, Vol. 12, Issue 4 https://doi.org/10.1007/s12042-019-09229-z	journal	June 2019
Comparison of leaf transcriptomes of cassava “Xinxuan 048” diploid and autotetraploid plants Yin, Ling; Qu, Junjie; Zhou, Huiwen Genes & Genomics, Vol. 40, Issue 9 https://doi.org/10.1007/s13258-018-0692-2	journal	May 2018
Cassava haplotype map highlights fixation of deleterious mutations during clonal propagation Ramu, Punna; Esuma, Williams; Kawuki, Robert Nature Genetics, Vol. 49, Issue 6 https://doi.org/10.1038/ng.3845	journal	April 2017
The evolutionary significance of polyploidy Van de Peer, Yves; Mizrachi, Eshchar; Marchal, Kathleen Nature Reviews Genetics, Vol. 18, Issue 7 https://doi.org/10.1038/nrg.2017.26	journal	May 2017
Allele-defined genome of the autopolyploid sugarcane Saccharum spontaneum L. Zhang, Jisen; Zhang, Xingtan; Tang, Haibao Nature Genetics, Vol. 50, Issue 11 https://doi.org/10.1038/s41588-018-0237-2	journal	October 2018
Genome-wide association mapping and genomic prediction for CBSD resistance in Manihot esculenta Kayondo, Siraj Ismail; Pino Del Carpio, Dunia; Lozano, Roberto Scientific Reports, Vol. 8, Issue 1 https://doi.org/10.1038/s41598-018-19696-1	journal	January 2018
A high-quality genome of Eragrostis curvula grass provides insights into Poaceae evolution and supports new strategies to enhance forage quality Carballo, J.; Santos, B. A. C. M.; Zappacosta, D. Scientific Reports, Vol. 9, Issue 1 https://doi.org/10.1038/s41598-019-46610-0	journal	July 2019
Reproductive biology in cassava: stigma receptivity and pollen tube growth Ramos Abril, L. N.; Pineda, L. M.; Wasek, I. Communicative & Integrative Biology, Vol. 12, Issue 1 https://doi.org/10.1080/19420889.2019.1631110	journal	January 2019
Phylogenetic approaches to identifying fragments of the same gene, with application to the wheat genome Piližota, Ivana; Train, Clément-Marie; Altenhoff, Adrian Bioinformatics, Vol. 35, Issue 7 https://doi.org/10.1093/bioinformatics/bty772	journal	September 2018
Evolutionary history and structure of nuclear matrix constituent proteins, the plant analogues of lamins Ciska, Malgorzata; Hikida, Riku; Masuda, Kiyoshi Journal of Experimental Botany, Vol. 70, Issue 10 https://doi.org/10.1093/jxb/erz102	journal	March 2019
Symplasmic phloem unloading and radial post-phloem transport via vascular rays in tuberous roots of Manihot esculenta Mehdi, Rabih; Lamm, Christian E.; Bodampalli Anjanappa, Ravi Journal of Experimental Botany, Vol. 70, Issue 20 https://doi.org/10.1093/jxb/erz297	journal	June 2019
Dual RNA-seq reveals large-scale non-conserved genotype x genotype specific genetic reprograming and molecular crosstalk in the mycorrhizal symbiosis Mateus, Ivan D.; Masclaux, Frédéric G.; Aletti, Consolée https://doi.org/10.1101/393637	posted_content	December 2018
Production of very‐high‐amylose cassava by post‐transcriptional silencing of branching enzyme genes Zhou, Wenzhi; Zhao, Shanshan; He, Shutao Journal of Integrative Plant Biology, Vol. 62, Issue 6 https://doi.org/10.1111/jipb.12848	journal	September 2019
Rooting for cassava: insights into photosynthesis and associated physiology as a route to improve yield potential De Souza, Amanda P.; Massenburg, Lynnicia N.; Jaiswal, Deepak New Phytologist, Vol. 213, Issue 1 https://doi.org/10.1111/nph.14250	journal	October 2016
Widespread ancient whole-genome duplications in Malpighiales coincide with Eocene global climatic upheaval Cai, Liming; Xi, Zhenxiang; Amorim, André M. New Phytologist, Vol. 221, Issue 1 https://doi.org/10.1111/nph.15357	journal	July 2018
The extent of adaptive wild introgression in crops Janzen, Garrett M.; Wang, Li; Hufford, Matthew B. New Phytologist, Vol. 221, Issue 3 https://doi.org/10.1111/nph.15457	journal	October 2018
Photosynthesis across African cassava germplasm is limited by Rubisco and mesophyll conductance at steady state, but by stomatal conductance in fluctuating light De Souza, Amanda P.; Wang, Yu; Orr, Douglas J. New Phytologist, Vol. 225, Issue 6 https://doi.org/10.1111/nph.16142	journal	October 2019
Divergence of duplicated genes by repeated partitioning of splice forms and subcellular localization Qiu, Yichun; Tay, Yii Van; Ruan, Yuan New Phytologist, Vol. 225, Issue 2 https://doi.org/10.1111/nph.16148	journal	October 2019
Provitamin A biofortification of cassava enhances shelf life but reduces dry matter content of storage roots due to altered carbon partitioning into starch Beyene, Getu; Solomon, Felix R.; Chauhan, Raj D. Plant Biotechnology Journal, Vol. 16, Issue 6 https://doi.org/10.1111/pbi.12862	journal	December 2017
Allele exchange at the EPSPS locus confers glyphosate tolerance in cassava Hummel, Aaron W.; Chauhan, Raj Deepika; Cermak, Tomas Plant Biotechnology Journal, Vol. 16, Issue 7 https://doi.org/10.1111/pbi.12868	journal	January 2018
Simultaneous CRISPR/Cas9-mediated editing of cassava eIF4E isoforms nCBP-1 and nCBP-2 reduces cassava brown streak disease symptom severity and incidence Gomez, Michael A.; Lin, Z. Daniel; Moll, Theodore Plant Biotechnology Journal, Vol. 17, Issue 2 https://doi.org/10.1111/pbi.12987	journal	October 2018
Metabolite database for root, tuber, and banana crops to facilitate modern breeding in understudied crops Price, Elliott J.; Drapal, Margit; Perez‐Fons, Laura The Plant Journal, Vol. 101, Issue 6 https://doi.org/10.1111/tpj.14649	journal	January 2020
Accelerated ex situ breeding of GBSS - and PTST1 -edited cassava for modified starch Bull, Simon E.; Seung, David; Chanez, Christelle Science Advances, Vol. 4, Issue 9 https://doi.org/10.1126/sciadv.aat6086	journal	September 2018
A statistical framework for detecting mislabeled and contaminated samples using shallow-depth sequence data Chan, Ariel W.; Williams, Amy L.; Jannink, Jean-Luc BMC Bioinformatics, Vol. 19, Issue 1 https://doi.org/10.1186/s12859-018-2512-8	journal	December 2018
Strategies for optimizing BioNano and Dovetail explored through a second reference quality assembly for the legume model, Medicago truncatula Moll, Karen M.; Zhou, Peng; Ramaraj, Thiruvarangan BMC Genomics, Vol. 18, Issue 1 https://doi.org/10.1186/s12864-017-3971-4	journal	August 2017
Genome-wide comparison reveals divergence of cassava and rubber aquaporin family genes after the recent whole-genome duplication Zou, Zhi; Yang, Jianghua BMC Genomics, Vol. 20, Issue 1 https://doi.org/10.1186/s12864-019-5780-4	journal	May 2019
Identification and validation of mutation points associated with waxy phenotype in cassava do Carmo, Cátia Dias; Sousa, Massaine Bandeira e.; dos Santos Silva, Priscila Patrícia BMC Plant Biology, Vol. 20, Issue 1 https://doi.org/10.1186/s12870-020-02379-3	journal	April 2020
Haplotype-resolved genomes of geminivirus-resistant and geminivirus-susceptible African cassava cultivars Kuon, Joel-E.; Qi, Weihong; Schläpfer, Pascal BMC Biology, Vol. 17, Issue 1 https://doi.org/10.1186/s12915-019-0697-6	journal	September 2019
A rapid virus-induced gene silencing (VIGS) method for assessing resistance and susceptibility to cassava mosaic disease Beyene, Getu; Chauhan, Raj Deepika; Taylor, Nigel J. Virology Journal, Vol. 14, Issue 1 https://doi.org/10.1186/s12985-017-0716-6	journal	March 2017
Cassava geminivirus agroclones for virus-induced gene silencing in cassava leaves and roots Lentz, Ezequiel Matias; Kuon, Joel-Elias; Alder, Adrian Plant Methods, Vol. 14, Issue 1 https://doi.org/10.1186/s13007-018-0340-5	journal	August 2018
Genomic approaches for studying crop evolution Schreiber, Mona; Stein, Nils; Mascher, Martin Genome Biology, Vol. 19, Issue 1 https://doi.org/10.1186/s13059-018-1528-8	journal	September 2018
PrediTALE: A novel model learned from quantitative data allows for new perspectives on TALE targeting Erkes, Annett; Mücke, Stefanie; Reschke, Maik PLOS Computational Biology, Vol. 15, Issue 7 https://doi.org/10.1371/journal.pcbi.1007206	journal	July 2019
Proteomic analysis of injured storage roots in cassava (Manihot esculenta Crantz) under postharvest physiological deterioration Qin, Yuling; Djabou, Astride Stéphanie Mouafi; An, Feifei PLOS ONE, Vol. 12, Issue 3 https://doi.org/10.1371/journal.pone.0174238	journal	March 2017
Molecular diversity analysis, drought related marker-traits association mapping and discovery of excellent alleles for 100-day old plants by EST-SSRs in cassava germplasms (Manihot esculenta Cranz) Wang, Bin; Guo, Xin; Zhao, Pingjuan PLOS ONE, Vol. 12, Issue 5 https://doi.org/10.1371/journal.pone.0177456	journal	May 2017
Overexpression of Arabidopsis FLOWERING LOCUS T (FT) gene improves floral development in cassava (Manihot esculenta, Crantz) Adeyemo, O. Sarah; Chavarriaga, Paul; Tohme, Joe PLOS ONE, Vol. 12, Issue 7 https://doi.org/10.1371/journal.pone.0181460	journal	July 2017
A global overview of cassava genetic diversity Ferguson, Morag E.; Shah, Trushar; Kulakow, Peter PLOS ONE, Vol. 14, Issue 11 https://doi.org/10.1371/journal.pone.0224763	journal	November 2019
Genome‐Wide Association Study of Resistance to Cassava Green Mite Pest and Related Traits in Cassava Ezenwaka, Lydia; Carpio, Dunia Pino; Jannink, Jean-Luc Crop Science, Vol. 58, Issue 5 https://doi.org/10.2135/cropsci2018.01.0024	journal	July 2018
QTL Mapping for Pest and Disease Resistance in Cassava and Coincidence of Some QTL with Introgression Regions Derived from Manihot glaziovii Nzuki, Inosters; Katari, Manpreet S.; Bredeson, Jessen V. Frontiers in Plant Science, Vol. 8 https://doi.org/10.3389/fpls.2017.01168	journal	July 2017
Genome-Wide Identification and Expression, Protein–Protein Interaction and Evolutionary Analysis of the Seed Plant-Specific BIG GRAIN and BIG GRAIN LIKE Gene Family Mishra, Bhuwaneshwar S.; Jamsheer K., Muhammed; Singh, Dhriti Frontiers in Plant Science, Vol. 8 https://doi.org/10.3389/fpls.2017.01812	journal	October 2017
Adaptive Introgression: An Untapped Evolutionary Mechanism for Crop Adaptation Burgarella, Concetta; Barnaud, Adeline; Kane, Ndjido Ardo Frontiers in Plant Science, Vol. 10 https://doi.org/10.3389/fpls.2019.00004	journal	February 2019
Genomics-Assisted Breeding in the CGIAR Research Program on Roots, Tubers and Bananas (RTB) Friedmann, Michael; Asfaw, Asrat; Anglin, Noelle Agriculture, Vol. 8, Issue 7 https://doi.org/10.3390/agriculture8070089	journal	June 2018
Genome-Wide Identification, Expression, and Functional Analysis of the Sugar Transporter Gene Family in Cassava (Manihot esculenta) Liu, Qin; Dang, Huijie; Chen, Zhijian International Journal of Molecular Sciences, Vol. 19, Issue 4 https://doi.org/10.3390/ijms19040987	journal	March 2018
Genome‐Wide Association Mapping of Correlated Traits in Cassava: Dry Matter and Total Carotenoid Content Rabbi, Ismail Y.; Udoh, Lovina I.; Wolfe, Marnin The Plant Genome, Vol. 10, Issue 3 https://doi.org/10.3835/plantgenome2016.09.0094	journal	November 2017
Symplasmic phloem unloading and radial post-phloem transport via vascular rays in tuberous roots of Manihot esculenta Rabih, Mehdi,; E., Lamm, Christian; B., Anjanappa, Ravi ETH Zurich https://doi.org/10.3929/ethz-b-000374960	text	January 2019
Characterization of introgression from the teosinte Zea mays ssp. mexicana to Mexican highland maize Gonzalez-Segovia, Eric; Pérez-Limon, Sergio; Cíntora-Martínez, G. Carolina PeerJ, Vol. 7 https://doi.org/10.7717/peerj.6815	journal	January 2019