Minimally disruptive optical control of protein tyrosine phosphatase 1B

Hongdusit, Akarawin; Zwart, Peter H.; Sankaran, Banumathi; Fox, Jerome M.

doi:10.1038/s41467-020-14567-8

Minimally disruptive optical control of protein tyrosine phosphatase 1B

Journal Article · Fri Feb 07 00:00:00 EST 2020 · Nature Communications

DOI:https://doi.org/10.1038/s41467-020-14567-8· OSTI ID:1615301

Hongdusit, Akarawin ^[1]; Zwart, Peter H. ^[2]; Sankaran, Banumathi ^[2]; ^[1]

Univ. of Colorado, Boulder, CO (United States)
Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)

Protein tyrosine phosphatases regulate a myriad of essential subcellular signaling events, yet they remain difficult to study in their native biophysical context. Here we develop a minimally disruptive optical approach to control protein tyrosine phosphatase 1B (PTP1B)—an important regulator of receptor tyrosine kinases and a therapeutic target for the treatment of diabetes, obesity, and cancer—and we use that approach to probe the intracellular function of this enzyme. Our conservative architecture for photocontrol, which consists of a protein-based light switch fused to an allosteric regulatory element, preserves the native structure, activity, and subcellular localization of PTP1B, affords changes in activity that match those elicited by post-translational modifications inside the cell, and permits experimental analyses of the molecular basis of optical modulation. Findings indicate, most strikingly, that small changes in the activity of PTP1B can cause large shifts in the phosphorylation states of its regulatory targets.

View Accepted Manuscript (DOE)

Research Organization:: Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)

Sponsoring Organization:: National Institutes of Health (NIH); National Science Foundation (NSF); USDOE Office of Science (SC)

Grant/Contract Number:: AC02-05CH11231

OSTI ID:: 1615301

Journal Information:: Nature Communications, Journal Name: Nature Communications Journal Issue: 1 Vol. 11; ISSN 2041-1723

Publisher:: Nature Publishing GroupCopyright Statement

Country of Publication:: United States

Language:: English

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