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Ozone Gas: Scientific Justification and Practical Guidelines for Improvised Disinfection using Consumer-Grade Ozone Generators and Plastic Storage Boxes

Journal Article · · Journal of Science and Medicine
 [1];  [2];  [1];  [1]
  1. Univ. of North Carolina, Chapel Hill, NC (United States)
  2. Sandia National Lab. (SNL-CA), Livermore, CA (United States)

Here, we describe direct measurements of ozone concentration achievable in small enclosed containers (plastic storage boxes) for use as improvised decontamination systems for small articles such as disposable PPE (N95 masks, nitrile gloves, etc.), clothing, mail and small packages, food, and other miscellaneous articles. The emphasis is on the reliable and sustained generation of ozone gas concentrations of sufficient concentration and duration to create an effective virucidal environment to achieve more than 95% to 99% viral inactivation, based upon the data already published in the peer-review literature on this topic. The suggestion that ozone be used to inactivate virus is certainly not a new idea. Our objective in this report is to make clear that the necessary levels of ozone can be improvised using simple, easy-to-use, inexpensive, and widely available supplies, and that there is every theoretical and experimental reason to believe that this approach is as highly effective in viral inactivation by ozone as are the far more expensive, complex, cumbersome, and less available equivalent ozone (and other) disinfectant systems that have themselves become unavailable during times of pandemic crisis. Using multiple types of readily available commercial ozone generators, concentration in the tested improvised enclosure is tracked over time to assess ozone charging and decay rates, and the ozone quenching effects of items placed in the box. Generator performance is compared against published ozone dosage values for virucidal and antimicrobial activity. Bubbler and box-fan-type ozone generators were found to be effective at achieving and maintaining target concentrations of 10ppm ozone or higher, whereas automotive cigarette lighter and universal serial bus type plug in “air freshener” ozone generators could not achieve the target concentrations in these experiments. Calculations and practical guidelines for assembly and effective use of an ozone box for improvised decontamination are offered. The majority of this report is directed toward the scientific justification and rationale for this approach. The end of the document summarizes the findings and offers simplified designs for the construction and use of ozone boxes as an improvised method of disinfection.

Research Organization:
Sandia National Laboratories (SNL-NM), Albuquerque, NM (United States)
Sponsoring Organization:
USDOE National Nuclear Security Administration (NNSA)
Grant/Contract Number:
AC04-94AL85000; NA0003525
OSTI ID:
1614788
Report Number(s):
SAND--2020-3866J; 685214
Journal Information:
Journal of Science and Medicine, Journal Name: Journal of Science and Medicine Journal Issue: 1 Vol. 2; ISSN 2690-2656
Publisher:
Cortical Metrics LLCCopyright Statement
Country of Publication:
United States
Language:
English

References (10)

Ozone Disinfection journal March 1972
Inactivation Kinetics of Viruses and Bacteria in Water by Use of Ozone journal December 1974
Latex condom deterioration accelerated by environmental factors: I. Ozone journal March 1989
Comparative response of mixed cultures of bacteria and virus to ozonation journal January 1983
Kinetics of Inactivation of Waterborne Enteric Viruses by Ozone journal January 2018
Degradation of materials in the atmosphere journal November 1986
Ozone for Inactivation of Aerosolized Bacteriophages journal August 2006
Surface Germicidal Effects of Ozone for Microorganisms journal July 2003
Evaluating resistance of polymeric materials for outdoor applications to corona and ozone journal April 2010
Mechanism of enteroviral inactivation by ozone. journal January 1981

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