Phosphite binding by the HtxB periplasmic binding protein depends on the protonation state of the ligand

Adams, Nathan B.  P.; Robertson, Angus J.; Hunter, C. Neil; Hitchcock, Andrew; Bisson, Claudine

doi:10.1038/s41598-019-46557-2

Title: Phosphite binding by the HtxB periplasmic binding protein depends on the protonation state of the ligand

Journal Article · Mon Jul 15 00:00:00 EDT 2019 · Scientific Reports

DOI:https://doi.org/10.1038/s41598-019-46557-2· OSTI ID:1610494

^[1]; Robertson, Angus J. ^[1];

^[1]; Hitchcock, Andrew ^[1];

^[2]

Univ. of Sheffield (United Kingdom)
Univ. of Sheffield (United Kingdom); Univ. of London (United Kingdom). Birkbeck College

Phosphorus acquisition is critical for life. In low phosphate conditions, some species of bacteria have evolved mechanisms to import reduced phosphorus compounds, such as phosphite and hypophosphite, as alternative phosphorus sources. Uptake is facilitated by high-affinity periplasmic binding proteins (PBPs) that bind cargo in the periplasm and shuttle it to an ATP-binding cassette (ABC)-transporter in the bacterial inner membrane. PtxB and HtxB are the PBPs responsible for binding phosphite and hypophosphite, respectively. They recognize the P-H bond of phosphite/hypophosphite via a conserved P-H...π interaction, which confers nanomolar dissociation constants for their respective ligands. PtxB also has a low-level binding affinity for phosphate and hypophosphite, whilst HtxB can facilitate phosphite uptake in vivo. However, HtxB does not bind phosphate, thus the HtxBCDE transporter has recently been successfully exploited for biocontainment of genetically modified organisms by phosphite-dependent growth. Here we use a combination of X-ray crystallography, NMR and Microscale Thermophoresis to show that phosphite binding to HtxB depends on the protonation state of the ligand, suggesting that pH may effect the efficiency of phosphite uptake by HtxB in biotechnology applications.

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Research Organization:: Washington Univ., St. Louis, MO (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Basic Energy Sciences (BES); Biotechnology and Biological Sciences Research Council (BBSRC), United Kingdom; European Research Council (ERC)

Grant/Contract Number:: SC0001035

OSTI ID:: 1610494

Journal Information:: Scientific Reports, Vol. 9, Issue 1; ISSN 2045-2322

Publisher:: Nature Publishing GroupCopyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 6 works

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