Rational Design Principles for the Transport and Subcellular Distribution of Nanomaterials into Plant Protoplasts

Lew, Tedrick Thomas Salim; Wong, Min Hao; Kwak, Seon-Yeong; Sinclair, Rosalie; Koman, Volodymyr B.; Strano, Michael S.

doi:10.1002/smll.201802086

Title: Rational Design Principles for the Transport and Subcellular Distribution of Nanomaterials into Plant Protoplasts

Journal Article · Thu Sep 06 00:00:00 EDT 2018 · Small

DOI:https://doi.org/10.1002/smll.201802086· OSTI ID:1609981

Lew, Tedrick Thomas Salim ^[1]; Wong, Min Hao ^[1]; Kwak, Seon-Yeong ^[1]; Sinclair, Rosalie ^[1]; Koman, Volodymyr B. ^[1]; Strano, Michael S. ^[1]

Massachusetts Inst. of Technology (MIT), Cambridge, MA (United States)

Abstract The ability to control the subcellular localization of nanoparticles within living plants offers unique advantages for targeted biomolecule delivery and enables important applications in plant bioengineering. However, the mechanism of nanoparticle transport past plant biological membranes is poorly understood. Here, a mechanistic study of nanoparticle cellular uptake into plant protoplasts is presented. An experimentally validated mathematical model of lipid exchange envelope penetration mechanism for protoplasts, which predicts that the subcellular distribution of nanoparticles in plant cells is dictated by the particle size and the magnitude of the zeta potential, is advanced. The mechanism is completely generic, describing nanoparticles ranging from quantum dots, gold and silica nanoparticles, nanoceria, and single‐walled carbon nanotubes (SWNTs). In addition, the use of imaging flow cytometry to investigate the influence of protoplasts' morphological characteristics on nanoparticle uptake efficiency is demonstrated. Using DNA‐wrapped SWNTs as model nanoparticles, it is found that glycerolipids, the predominant lipids in chloroplast membranes, exhibit stronger lipid–nanoparticle interaction than phospholipids, the major constituent in protoplast membrane. This work can guide the rational design of nanoparticles for targeted delivery into specific compartments within plant cells without the use of chemical or mechanical aid, potentially enabling various plant engineering applications.

View Accepted Manuscript (DOE)

View Accepted Manuscript (Publisher)

Cite

Export

Save

Research Organization:: Massachusetts Inst. of Technology (MIT), Cambridge, MA (United States)

Sponsoring Organization:: USDOE Office of Science (SC)

Grant/Contract Number:: FG02-08ER46488; DE‐FG02‐08ER46488 Mod 0008

OSTI ID:: 1609981

Alternate ID(s):: OSTI ID: 1469026

Journal Information:: Small, Vol. 14, Issue 44; ISSN 1613-6810

Publisher:: WileyCopyright Statement

Country of Publication:: United States

Language:: English

Citation Metrics:

Cited by: 70 works

Citation information provided by
Web of Science

References (87)

Imaging Flow Cytometry: Coping with Heterogeneity in Biological Systems Barteneva, Natasha S.; Fasler-Kan, Elizaveta; Vorobjev, Ivan A. Journal of Histochemistry & Cytochemistry, Vol. 60, Issue 10 https://doi.org/10.1369/0022155412453052	journal	June 2012
Ultrasmall Gold Nanoparticles as Carriers for Nucleus-Based Gene Therapy Due to Size-Dependent Nuclear Entry Huo, Shuaidong; Jin, Shubin; Ma, Xiaowei ACS Nano, Vol. 8, Issue 6 https://doi.org/10.1021/nn5008572	journal	May 2014
Surface-structure-regulated cell-membrane penetration by monolayer-protected nanoparticles Verma, Ayush; Uzun, Oktay; Hu, Yuhua Nature Materials, Vol. 7, Issue 7 https://doi.org/10.1038/nmat2202	journal	May 2008
In vitro transformation of plant protoplasts with Ti-plasmid DNA Krens, F. A.; Molendijk, L.; Wullems, G. J. Nature, Vol. 296, Issue 5852 https://doi.org/10.1038/296072a0	journal	March 1982
Synthesis of Water-Soluble Blue Photoluminescent Silicon Nanocrystals with Oxide Surface Passivation Lin, Sheng-Wei; Chen, Dong-Hwang Small, Vol. 5, Issue 1 https://doi.org/10.1002/smll.200800677	journal	January 2009
Self-Assembly of Lipids and Single-Walled Carbon Nanotubes: Effects of Lipid Structure and PEGylation Lee, Hwankyu; Kim, Hyungsu The Journal of Physical Chemistry C, Vol. 116, Issue 16 https://doi.org/10.1021/jp3010663	journal	April 2012
Biochemical and biophysical properties of thylakoid acyl lipids Webb, Murray S.; Green, Beverley R. Biochimica et Biophysica Acta (BBA) - Bioenergetics, Vol. 1060, Issue 2 https://doi.org/10.1016/S0005-2728(09)91002-7	journal	October 1991
Carbon Nanotubes as Molecular Transporters for Walled Plant Cells Liu, Qiaoling; Chen, Bo; Wang, Qinli Nano Letters, Vol. 9, Issue 3 https://doi.org/10.1021/nl803083u	journal	March 2009
Electroporation-mediated infection of tobacco leaf protoplasts with tobacco mosaic virus RNA and cucumber mosaic virus RNA Nishiguchi, M.; Langridge, W. H. R.; Szalay, A. A. Plant Cell Reports, Vol. 5, Issue 1 https://doi.org/10.1007/BF00269719	journal	February 1986
Calcium phosphate nanoparticle mediated genetic transformation in plants Naqvi, Saba; Maitra, A. N.; Abdin, M. Z. Journal of Materials Chemistry, Vol. 22, Issue 8 https://doi.org/10.1039/c2jm11739h	journal	January 2012
Translocation of polyarginines and conjugated nanoparticles across asymmetric membranes Li, Zhen-lu; Ding, Hong-ming; Ma, Yu-qiang Soft Matter, Vol. 9, Issue 4 https://doi.org/10.1039/C2SM26519B	journal	January 2013
Designing Nanoparticle Translocation through Membranes by Computer Simulations Ding, Hong-ming; Tian, Wen-de; Ma, Yu-qiang ACS Nano, Vol. 6, Issue 2 https://doi.org/10.1021/nn2038862	journal	January 2012
Optical Detection of DNA Conformational Polymorphism on Single-Walled Carbon Nanotubes Heller, D. A. Science, Vol. 311, Issue 5760 https://doi.org/10.1126/science.1120792	journal	January 2006
Fluid Phase Endocytic Uptake of Artificial Nano-Spheres and Fluorescent Quantum Dots by Sycamore Cultured Cells: Evidence for the Distribution of Solutes to Different Intracellular Compartments Etxeberria, Ed; Gonzalez, Pedro; Baroja-Fernández, Edurne Plant Signaling & Behavior, Vol. 1, Issue 4 https://doi.org/10.4161/psb.1.4.3142	journal	July 2006
Functionalized Carbon Nanotubes for Plasmid DNA Gene Delivery Pantarotto, Davide; Singh, Ravi; McCarthy, David Angewandte Chemie International Edition, Vol. 43, Issue 39 https://doi.org/10.1002/anie.200460437	journal	October 2004
Plant nanobionic materials with a giant temperature response mediated by pectin-Ca ²⁺ Di Giacomo, Raffaele; Daraio, Chiara; Maresca, Bruno Proceedings of the National Academy of Sciences, Vol. 112, Issue 15 https://doi.org/10.1073/pnas.1421020112	journal	March 2015
Free Volume Properties of Sphingomyelin, DMPC, DPPC, and PLPC Bilayers Kupiainen, M.; Falck, E.; Ollila, S. Journal of Computational and Theoretical Nanoscience, Vol. 2, Issue 3 https://doi.org/10.1166/jctn.2005.211	journal	September 2005
Use of laurdan fluorescence intensity and polarization to distinguish between changes in membrane fluidity and phospholipid order Harris, Faith M.; Best, Katrina B.; Bell, John D. Biochimica et Biophysica Acta (BBA) - Biomembranes, Vol. 1565, Issue 1 https://doi.org/10.1016/S0005-2736(02)00514-X	journal	September 2002
A simple and effective method to encapsulate tobacco mesophyll protoplasts to maintain cell viability Lei, Rong; Qiao, Wenjie; Hu, Fan MethodsX, Vol. 2 https://doi.org/10.1016/j.mex.2014.11.004	journal	January 2015
Nanoparticle-induced surface reconstruction of phospholipid membranes Wang, B.; Zhang, L.; Bae, S. C. Proceedings of the National Academy of Sciences, Vol. 105, Issue 47 https://doi.org/10.1073/pnas.0807296105	journal	November 2008
Mechanical Properties of the Plasma Membrane of Isolated Plant Protoplasts: Mechanism of Hyperosmotic and Extracellular Freezing Injury Wolfe, Joe; Steponkus, Peter L. Plant Physiology, Vol. 71, Issue 2 https://doi.org/10.1104/pp.71.2.276	journal	February 1983
Non-endocytic penetration of core histones into petunia protoplasts and cultured cells: a novel mechanism for the introduction of macromolecules into plant cells Rosenbluh, Joseph; Singh, Sunil Kumar; Gafni, Yedidya Biochimica et Biophysica Acta (BBA) - Biomembranes, Vol. 1664, Issue 2 https://doi.org/10.1016/j.bbamem.2004.06.003	journal	August 2004
Chloroplast envelope membranes: a dynamic interface between plastids and the cytosol Block, Maryse A.; Douce, Roland; Joyard, Jacques Photosynthesis Research, Vol. 92, Issue 2 https://doi.org/10.1007/s11120-007-9195-8	journal	June 2007
Transient transformation of Arabidopsis leaf protoplasts: a versatile experimental system to study gene expression Abel, Steffen; Theologis, Athanasios The Plant Journal, Vol. 5, Issue 3 https://doi.org/10.1111/j.1365-313X.1994.00421.x	journal	March 1994
Coating Single-Walled Carbon Nanotubes with Phospholipids Wu, Yonnie; Hudson, JoAn S.; Lu, Qi The Journal of Physical Chemistry B, Vol. 110, Issue 6 https://doi.org/10.1021/jp057252c	journal	February 2006
Organically modified silica nanoparticles: A nonviral vector for in vivo gene delivery and expression in the brain Bharali, D. J.; Klejbor, I.; Stachowiak, E. K. Proceedings of the National Academy of Sciences, Vol. 102, Issue 32 https://doi.org/10.1073/pnas.0504926102	journal	July 2005
A Polyethylene Glycol-Mediated Protoplast Transformation System for Production of Fertile Transgenic Rice Plants Hayashimoto, Akio; Li, Zhijian; Murai, Norimoto Plant Physiology, Vol. 93, Issue 3 https://doi.org/10.1104/pp.93.3.857	journal	July 1990
Molecular biology of the plasma membrane of higher plants. Sussman, M. R.; Harper, J. F. The Plant Cell, Vol. 1, Issue 10 https://doi.org/10.1105/tpc.1.10.953	journal	October 1989
Homogeneous Hydrophobic–Hydrophilic Surface Patterns Enhance Permeation of Nanoparticles through Lipid Membranes Gkeka, Paraskevi; Sarkisov, Lev; Angelikopoulos, Panagiotis The Journal of Physical Chemistry Letters, Vol. 4, Issue 11 https://doi.org/10.1021/jz400679z	journal	May 2013
Protein-targeted corona phase molecular recognition Bisker, Gili; Dong, Juyao; Park, Hoyoung D. Nature Communications, Vol. 7, Issue 1 https://doi.org/10.1038/ncomms10241	journal	January 2016
Surface Patterning of Carbon Nanotubes Can Enhance Their Penetration through a Phospholipid Bilayer Pogodin, Sergey; Slater, Nigel K. H.; Baulin, Vladimir A. ACS Nano, Vol. 5, Issue 2 https://doi.org/10.1021/nn102763b	journal	January 2011
On The Mechanical and Dynamic Properties of Plant Cell Membranes: Their Role in Growth, Direct Gene Transfer and Protoplast Fusion Kell, Antje; Glaser, Ralf W. Journal of Theoretical Biology, Vol. 160, Issue 1 https://doi.org/10.1006/jtbi.1993.1003	journal	January 1993
Interparticle Dispersion, Membrane Curvature, and Penetration Induced by Single-Walled Carbon Nanotubes Wrapped with Lipids and PEGylated Lipids Lee, Hwankyu The Journal of Physical Chemistry B, Vol. 117, Issue 5 https://doi.org/10.1021/jp308912r	journal	January 2013
Bioconjugated Nanoparticles for DNA Protection from Cleavage He, Xiao-xiao; Wang, Kemin; Tan, Weihong Journal of the American Chemical Society, Vol. 125, Issue 24 https://doi.org/10.1021/ja034450d	journal	June 2003
Plant lipids and their role in membrane function Quinn, P. J.; Williams, W. P. Progress in Biophysics and Molecular Biology, Vol. 34 https://doi.org/10.1016/0079-6107(79)90016-6	journal	January 1979
Enhanced Gene Delivery and siRNA Silencing by Gold Nanoparticles Coated with Charge-Reversal Polyelectrolyte Guo, Shutao; Huang, Yuanyu; Jiang, Qiao ACS Nano, Vol. 4, Issue 9 https://doi.org/10.1021/nn101638u	journal	August 2010
Spectroscopy of Single- and Double-Wall Carbon Nanotubes in Different Environments Hertel, Tobias; Hagen, Axel; Talalaev, Vadim Nano Letters, Vol. 5, Issue 3 https://doi.org/10.1021/nl050069a	journal	March 2005
Role of the Plasma Membrane in Freezing Injury and Cold Acclimation Steponkus, P. L. Annual Review of Plant Physiology, Vol. 35, Issue 1 https://doi.org/10.1146/annurev.pp.35.060184.002551	journal	June 1984
Molecular Insight into the Line Tension of Bilayer Membranes Containing Hybrid Polyunsaturated Lipids Rosetti, Carla M.; Montich, Guillermo G.; Pastorino, Claudio The Journal of Physical Chemistry B, Vol. 121, Issue 7 https://doi.org/10.1021/acs.jpcb.6b10836	journal	February 2017
Enhancement of biomolecule transport by electroporation: A review of theory and practical application to transformation ofCorynebacterium glutamicum Tryfona, Theodora; Bustard, Mark T. Biotechnology and Bioengineering, Vol. 93, Issue 3 https://doi.org/10.1002/bit.20725	journal	January 2006
Plant nanobionics approach to augment photosynthesis and biochemical sensing Giraldo, Juan Pablo; Landry, Markita P.; Faltermeier, Sean M. Nature Materials, Vol. 13, Issue 4 https://doi.org/10.1038/nmat3890	journal	March 2014
One Protoplast Is Not the Other! Faraco, Marianna; Di Sansebastiano, Gian Pietro; Spelt, Kees Plant Physiology, Vol. 156, Issue 2 https://doi.org/10.1104/pp.111.173708	journal	March 2011
Debundling of Single-Walled Nanotubes by Dilution: Observation of Large Populations of Individual Nanotubes in Amide Solvent Dispersions Giordani, Silvia; Bergin, Shane D.; Nicolosi, Valeria The Journal of Physical Chemistry B, Vol. 110, Issue 32 https://doi.org/10.1021/jp0626216	journal	August 2006
Signal Transduction in Maize and Arabidopsis Mesophyll Protoplasts Sheen, Jen Plant Physiology, Vol. 127, Issue 4 https://doi.org/10.1104/pp.010820	journal	December 2001
Determination of the Pore Size of Cell Walls of Living Plant Cells Carpita, N.; Sabularse, D.; Montezinos, D. Science, Vol. 205, Issue 4411 https://doi.org/10.1126/science.205.4411.1144	journal	September 1979
Nanoparticle-Induced Permeability of Lipid Membranes Pogodin, Sergey; Werner, Marco; Sommer, Jens-Uwe ACS Nano, Vol. 6, Issue 12 https://doi.org/10.1021/nn3028858	journal	November 2012
Cold Acclimation of Arabidopsis thaliana (Effect on Plasma Membrane Lipid Composition and Freeze-Induced Lesions) Uemura, M.; Joseph, R. A.; Steponkus, P. L. Plant Physiology, Vol. 109, Issue 1 https://doi.org/10.1104/pp.109.1.15	journal	September 1995
Nanosensor Technology Applied to Living Plant Systems Kwak, Seon-Yeong; Wong, Min Hao; Lew, Tedrick Thomas Salim Annual Review of Analytical Chemistry, Vol. 10, Issue 1 https://doi.org/10.1146/annurev-anchem-061516-045310	journal	June 2017
Vehicles and ways for efficient nuclear transformation in plants Husaini, Amjad Masood; Abdin, Mallik Zainul; Parray, Ghulam Ahmad GM Crops, Vol. 1, Issue 5 https://doi.org/10.4161/gmcr.1.5.14660	journal	November 2010
A Nanobionic Light-Emitting Plant Kwak, Seon-Yeong; Giraldo, Juan Pablo; Wong, Min Hao Nano Letters, Vol. 17, Issue 12 https://doi.org/10.1021/acs.nanolett.7b04369	journal	November 2017
Arabidopsis mesophyll protoplasts: a versatile cell system for transient gene expression analysis Yoo, Sang-Dong; Cho, Young-Hee; Sheen, Jen Nature Protocols, Vol. 2, Issue 7 https://doi.org/10.1038/nprot.2007.199	journal	June 2007
Efficient metabolic pathway engineering in transgenic tobacco and tomato plastids with synthetic multigene operons Lu, Y.; Rijzaani, H.; Karcher, D. Proceedings of the National Academy of Sciences, Vol. 110, Issue 8 https://doi.org/10.1073/pnas.1216898110	journal	February 2013
Physical Trauma and Tungsten Toxicity Reduce the Efficiency of Biolistic Transformation Russell, Julie A.; Roy, Mihir K.; Sanford, John C. Plant Physiology, Vol. 98, Issue 3 https://doi.org/10.1104/pp.98.3.1050	journal	March 1992
A simple plant gene delivery system using mesoporous silica nanoparticles as carriers Chang, Feng-Peng; Kuang, Lin-Yun; Huang, Chia-An Journal of Materials Chemistry B, Vol. 1, Issue 39 https://doi.org/10.1039/c3tb20529k	journal	January 2013
Chemical Basis of Interactions Between Engineered Nanoparticles and Biological Systems Mu, Qingxin; Jiang, Guibin; Chen, Lingxin Chemical Reviews, Vol. 114, Issue 15 https://doi.org/10.1021/cr400295a	journal	June 2014
Lipid Exchange Envelope Penetration (LEEP) of Nanoparticles for Plant Engineering: A Universal Localization Mechanism Wong, Min Hao; Misra, Rahul P.; Giraldo, Juan P. Nano Letters, Vol. 16, Issue 2 https://doi.org/10.1021/acs.nanolett.5b04467	journal	January 2016
Root Uptake and Phytotoxicity of ZnO Nanoparticles Lin, Daohui; Xing, Baoshan Environmental Science & Technology, Vol. 42, Issue 15 https://doi.org/10.1021/es800422x	journal	August 2008
Polyethylene glycol and electric field treatment of plant protoplasts: characterization of some membrane properties Hahn-Hagerdal, Barbel; Hosono, Kuniaki; Zaehrisson, Anders Physiologia Plantarum, Vol. 67, Issue 3 https://doi.org/10.1111/j.1399-3054.1986.tb05748.x	journal	July 1986
Functional Platform for Controlled Subcellular Distribution of Carbon Nanotubes Serag, Maged F.; Kaji, Noritada; Venturelli, Enrica ACS Nano, Vol. 5, Issue 11 https://doi.org/10.1021/nn2035654	journal	October 2011
SoyDB: a knowledge database of soybean transcription factors Wang, Zheng; Libault, Marc; Joshi, Trupti BMC Plant Biology, Vol. 10, Issue 1 https://doi.org/10.1186/1471-2229-10-14	journal	January 2010
Urea-Hydroxyapatite Nanohybrids for Slow Release of Nitrogen Kottegoda, Nilwala; Sandaruwan, Chanaka; Priyadarshana, Gayan ACS Nano, Vol. 11, Issue 2 https://doi.org/10.1021/acsnano.6b07781	journal	January 2017
Assessment of cell wall porosity in Arabidopsis thaliana by NMR spectroscopy Rondeau-Mouro, C.; Defer, D.; Leboeuf, E. International Journal of Biological Macromolecules, Vol. 42, Issue 2 https://doi.org/10.1016/j.ijbiomac.2007.09.020	journal	March 2008
<span class="fa fa-external-link" aria-hidden="true"></span></a> <ul class="small references-list mb-0 mt-0" style="list-style-type:none; padding-left: 0; line-height:1.8em;"> <li class="authors">Wanichapichart, Pikul; Bunthawin, Sarawuth; Kaewpaiboon, Amnuoy</li> <li class="mb-0-5 source"> <span class="mr-1">ScienceAsia, Vol. 28, Issue 2</span> <span class="text-muted"><a href="https://doi.org/10.2306/scienceasia1513-1874.2002.28.113" class="text-muted" target="_blank" rel="noopener noreferrer">https://doi.org/10.2306/scienceasia1513-1874.2002.28.113<span class="fa fa-external-link" aria-hidden="true"></span></a></span> </li> </ul> </td> <td class="small text-muted biblio-refs-td-secondary"> <span class="biblio-refs-type-badge ml-1 type" data-type="journal">journal</span> </td> <td class="small text-muted biblio-refs-td-secondary biblio-refs-td-secondary-date"><span class="date" data-date="2002-01-01">January 2002</span></td> </tr><tr> <td> <a href="https://doi.org/10.1038/nmat4771" target="_blank" rel="noopener noreferrer" class="title" data-title="Nitroaromatic detection and infrared communication from wildtype plants using plantnanobionics">Nitroaromatic detection and infrared communication from wild-type plants using plant nanobionics<span class="fa fa-external-link" aria-hidden="true"></span></a> <ul class="small references-list mb-0 mt-0" style="list-style-type:none; padding-left: 0; line-height:1.8em;"> <li class="authors">Wong, Min Hao; Giraldo, Juan P.; Kwak, Seon-Yeong</li> <li class="mb-0-5 source"> <span class="mr-1">Nature Materials, Vol. 16, Issue 2</span> <span class="text-muted"><a href="https://doi.org/10.1038/nmat4771" class="text-muted" target="_blank" rel="noopener noreferrer">https://doi.org/10.1038/nmat4771<span class="fa fa-external-link" aria-hidden="true"></span></a></span> </li> </ul> </td> <td class="small text-muted biblio-refs-td-secondary"> <span class="biblio-refs-type-badge ml-1 type" data-type="journal">journal</span> </td> <td class="small text-muted biblio-refs-td-secondary biblio-refs-td-secondary-date"><span class="date" data-date="2016-10-31">October 2016</span></td> </tr><tr> <td> <a href="https://doi.org/10.1016/j.cll.2007.05.008" target="_blank" rel="noopener noreferrer" class="title" data-title="Cellular Image Analysis and Imaging by Flow Cytometry">Cellular Image Analysis and Imaging by Flow Cytometry<span class="fa fa-external-link" aria-hidden="true"></span></a> <ul class="small references-list mb-0 mt-0" style="list-style-type:none; padding-left: 0; line-height:1.8em;"> <li class="authors">Basiji, David A.; Ortyn, William E.; Liang, Luchuan</li> <li class="mb-0-5 source"> <span class="mr-1">Clinics in Laboratory Medicine, Vol. 27, Issue 3</span> <span class="text-muted"><a href="https://doi.org/10.1016/j.cll.2007.05.008" class="text-muted" target="_blank" rel="noopener noreferrer">https://doi.org/10.1016/j.cll.2007.05.008<span class="fa fa-external-link" aria-hidden="true"></span></a></span> </li> </ul> </td> <td class="small text-muted biblio-refs-td-secondary"> <span class="biblio-refs-type-badge ml-1 type" data-type="journal">journal</span> </td> <td class="small text-muted biblio-refs-td-secondary biblio-refs-td-secondary-date"><span class="date" data-date="2007-09-01">September 2007</span></td> </tr><tr> <td> <a href="https://doi.org/10.1021/jp5024026" target="_blank" rel="noopener noreferrer" class="title" data-title="Cationic Au Nanoparticle Binding with Plasma Membranelike Lipid Bilayers Potential Mechanism for Spontaneous Permeation to Cells Revealed by Atomistic Simulations">Cationic Au Nanoparticle Binding with Plasma Membrane-like Lipid Bilayers: Potential Mechanism for Spontaneous Permeation to Cells Revealed by Atomistic Simulations<span class="fa fa-external-link" aria-hidden="true"></span></a> <ul class="small references-list mb-0 mt-0" style="list-style-type:none; padding-left: 0; line-height:1.8em;"> <li class="authors">Heikkilä, Elena; Martinez-Seara, Hector; Gurtovenko, Andrey A.</li> <li class="mb-0-5 source"> <span class="mr-1">The Journal of Physical Chemistry C, Vol. 118, Issue 20</span> <span class="text-muted"><a href="https://doi.org/10.1021/jp5024026" class="text-muted" target="_blank" rel="noopener noreferrer">https://doi.org/10.1021/jp5024026<span class="fa fa-external-link" aria-hidden="true"></span></a></span> </li> </ul> </td> <td class="small text-muted biblio-refs-td-secondary"> <span class="biblio-refs-type-badge ml-1 type" data-type="journal">journal</span> </td> <td class="small text-muted biblio-refs-td-secondary biblio-refs-td-secondary-date"><span class="date" data-date="2014-05-08">May 2014</span></td> </tr><tr> <td> <a href="https://doi.org/10.1038/78523" target="_blank" rel="noopener noreferrer" class="title" data-title="Enhancement of transfection by physical concentration of DNA at the cell surface">Enhancement of transfection by physical concentration of DNA at the cell surface<span class="fa fa-external-link" aria-hidden="true"></span></a> <ul class="small references-list mb-0 mt-0" style="list-style-type:none; padding-left: 0; line-height:1.8em;"> <li class="authors">Luo, Dan; Saltzman, W. Mark</li> <li class="mb-0-5 source"> <span class="mr-1">Nature Biotechnology, Vol. 18, Issue 8</span> <span class="text-muted"><a href="https://doi.org/10.1038/78523" class="text-muted" target="_blank" rel="noopener noreferrer">https://doi.org/10.1038/78523<span class="fa fa-external-link" aria-hidden="true"></span></a></span> </li> </ul> </td> <td class="small text-muted biblio-refs-td-secondary"> <span class="biblio-refs-type-badge ml-1 type" data-type="journal">journal</span> </td> <td class="small text-muted biblio-refs-td-secondary biblio-refs-td-secondary-date"><span class="date" data-date="2000-08-01">August 2000</span></td> </tr><tr> <td> <a href="https://doi.org/10.1021/jp076164k" target="_blank" rel="noopener noreferrer" class="title" data-title="Direct Synthesis of Nanoceria in Aqueous Polyhydroxyl Solutions">Direct Synthesis of Nanoceria in Aqueous Polyhydroxyl Solutions<span class="fa fa-external-link" aria-hidden="true"></span></a> <ul class="small references-list mb-0 mt-0" style="list-style-type:none; padding-left: 0; line-height:1.8em;"> <li class="authors">Karakoti, A. S.; Kuchibhatla, Satyanarayana V. N. T.; Babu, K. Suresh</li> <li class="mb-0-5 source"> <span class="mr-1">The Journal of Physical Chemistry C, Vol. 111, Issue 46</span> <span class="text-muted"><a href="https://doi.org/10.1021/jp076164k" class="text-muted" target="_blank" rel="noopener noreferrer">https://doi.org/10.1021/jp076164k<span class="fa fa-external-link" aria-hidden="true"></span></a></span> </li> </ul> </td> <td class="small text-muted biblio-refs-td-secondary"> <span class="biblio-refs-type-badge ml-1 type" data-type="journal">journal</span> </td> <td class="small text-muted biblio-refs-td-secondary biblio-refs-td-secondary-date"><span class="date" data-date="2007-10-10">October 2007</span></td> </tr><tr> <td> <a href="https://doi.org/10.1038/327070a0" target="_blank" rel="noopener noreferrer" class="title" data-title="Highvelocity microprojectiles for delivering nucleic acids into living cells">High-velocity microprojectiles for delivering nucleic acids into living cells<span class="fa fa-external-link" aria-hidden="true"></span></a> <ul class="small references-list mb-0 mt-0" style="list-style-type:none; padding-left: 0; line-height:1.8em;"> <li class="authors">Klein, T. M.; Wolf, E. D.; Wu, R.</li> <li class="mb-0-5 source"> <span class="mr-1">Nature, Vol. 327, Issue 6117, p. 70-73</span> <span class="text-muted"><a href="https://doi.org/10.1038/327070a0" class="text-muted" target="_blank" rel="noopener noreferrer">https://doi.org/10.1038/327070a0<span class="fa fa-external-link" aria-hidden="true"></span></a></span> </li> </ul> </td> <td class="small text-muted biblio-refs-td-secondary"> <span class="biblio-refs-type-badge ml-1 type" data-type="journal">journal</span> </td> <td class="small text-muted biblio-refs-td-secondary biblio-refs-td-secondary-date"><span class="date" data-date="1987-05-01">May 1987</span></td> </tr><tr> <td> <a href="https://doi.org/10.1021/nn203892h" target="_blank" rel="noopener noreferrer" class="title" data-title="Cellular Uptake of Nanoparticles by Membrane Penetration A Study Combining Confocal Microscopy with FTIR Spectroelectrochemistry">Cellular Uptake of Nanoparticles by Membrane Penetration: A Study Combining Confocal Microscopy with FTIR Spectroelectrochemistry<span class="fa fa-external-link" aria-hidden="true"></span></a> <ul class="small references-list mb-0 mt-0" style="list-style-type:none; padding-left: 0; line-height:1.8em;"> <li class="authors">Wang, Tiantian; Bai, Jing; Jiang, Xiue</li> <li class="mb-0-5 source"> <span class="mr-1">ACS Nano, Vol. 6, Issue 2</span> <span class="text-muted"><a href="https://doi.org/10.1021/nn203892h" class="text-muted" target="_blank" rel="noopener noreferrer">https://doi.org/10.1021/nn203892h<span class="fa fa-external-link" aria-hidden="true"></span></a></span> </li> </ul> </td> <td class="small text-muted biblio-refs-td-secondary"> <span class="biblio-refs-type-badge ml-1 type" data-type="journal">journal</span> </td> <td class="small text-muted biblio-refs-td-secondary biblio-refs-td-secondary-date"><span class="date" data-date="2012-01-17">January 2012</span></td> </tr><tr> <td> <a href="https://doi.org/10.1016/S0006-3495(92)81734-4" target="_blank" rel="noopener noreferrer" class="title" data-title="Dielectric spectroscopy of plant protoplasts">Dielectric spectroscopy of plant protoplasts<span class="fa fa-external-link" aria-hidden="true"></span></a> <ul class="small references-list mb-0 mt-0" style="list-style-type:none; padding-left: 0; line-height:1.8em;"> <li class="authors">Asami, Koji; Yamaguchi, Tohru</li> <li class="mb-0-5 source"> <span class="mr-1">Biophysical Journal, Vol. 63, Issue 6</span> <span class="text-muted"><a href="https://doi.org/10.1016/S0006-3495(92)81734-4" class="text-muted" target="_blank" rel="noopener noreferrer">https://doi.org/10.1016/S0006-3495(92)81734-4<span class="fa fa-external-link" aria-hidden="true"></span></a></span> </li> </ul> </td> <td class="small text-muted biblio-refs-td-secondary"> <span class="biblio-refs-type-badge ml-1 type" data-type="journal">journal</span> </td> <td class="small text-muted biblio-refs-td-secondary biblio-refs-td-secondary-date"><span class="date" data-date="1992-12-01">December 1992</span></td> </tr><tr> <td> <a href="https://doi.org/10.1073/pnas.82.17.5824" target="_blank" rel="noopener noreferrer" class="title" data-title="Expression of genes transferred into monocot and dicot plant cells by electroporation">Expression of genes transferred into monocot and dicot plant cells by electroporation.<span class="fa fa-external-link" aria-hidden="true"></span></a> <ul class="small references-list mb-0 mt-0" style="list-style-type:none; padding-left: 0; line-height:1.8em;"> <li class="authors">Fromm, M.; Taylor, L. P.; Walbot, V.</li> <li class="mb-0-5 source"> <span class="mr-1">Proceedings of the National Academy of Sciences, Vol. 82, Issue 17, p. 5824-5828</span> <span class="text-muted"><a href="https://doi.org/10.1073/pnas.82.17.5824" class="text-muted" target="_blank" rel="noopener noreferrer">https://doi.org/10.1073/pnas.82.17.5824<span class="fa fa-external-link" aria-hidden="true"></span></a></span> </li> </ul> </td> <td class="small text-muted biblio-refs-td-secondary"> <span class="biblio-refs-type-badge ml-1 type" data-type="journal">journal</span> </td> <td class="small text-muted biblio-refs-td-secondary biblio-refs-td-secondary-date"><span class="date" data-date="1985-09-01">September 1985</span></td> </tr><tr> <td> <a href="https://doi.org/10.1007/BF01870555" target="_blank" rel="noopener noreferrer" class="title" data-title="The thickness composition and structure of some lipid bilayers and natural membranes">The thickness, composition and structure of some lipid bilayers and natural membranes<span class="fa fa-external-link" aria-hidden="true"></span></a> <ul class="small references-list mb-0 mt-0" style="list-style-type:none; padding-left: 0; line-height:1.8em;"> <li class="authors">Fettiplace, R.; Andrews, D. M.; Haydon, D. A.</li> <li class="mb-0-5 source"> <span class="mr-1">The Journal of Membrane Biology, Vol. 5, Issue 3</span> <span class="text-muted"><a href="https://doi.org/10.1007/BF01870555" class="text-muted" target="_blank" rel="noopener noreferrer">https://doi.org/10.1007/BF01870555<span class="fa fa-external-link" aria-hidden="true"></span></a></span> </li> </ul> </td> <td class="small text-muted biblio-refs-td-secondary"> <span class="biblio-refs-type-badge ml-1 type" data-type="journal">journal</span> </td> <td class="small text-muted biblio-refs-td-secondary biblio-refs-td-secondary-date"><span class="date" data-date="1971-09-01">September 1971</span></td> </tr><tr> <td> <a href="https://doi.org/10.1007/s11427-012-4422-8" target="_blank" rel="noopener noreferrer" class="title" data-title="Highly efficient uptake of ultrafine mesoporous silica nanoparticles with excellent biocompatibility by Liriodendron hybrid suspension cells">Highly efficient uptake of ultrafine mesoporous silica nanoparticles with excellent biocompatibility by Liriodendron hybrid suspension cells<span class="fa fa-external-link" aria-hidden="true"></span></a> <ul class="small references-list mb-0 mt-0" style="list-style-type:none; padding-left: 0; line-height:1.8em;"> <li class="authors">Xia, Bing; Dong, Chen; Zhang, WenYi</li> <li class="mb-0-5 source"> <span class="mr-1">Science China Life Sciences, Vol. 56, Issue 1</span> <span class="text-muted"><a href="https://doi.org/10.1007/s11427-012-4422-8" class="text-muted" target="_blank" rel="noopener noreferrer">https://doi.org/10.1007/s11427-012-4422-8<span class="fa fa-external-link" aria-hidden="true"></span></a></span> </li> </ul> </td> <td class="small text-muted biblio-refs-td-secondary"> <span class="biblio-refs-type-badge ml-1 type" data-type="journal">journal</span> </td> <td class="small text-muted biblio-refs-td-secondary biblio-refs-td-secondary-date"><span class="date" data-date="2012-12-20">December 2012</span></td> </tr><tr> <td> <a href="https://doi.org/10.1007/s002320010040" target="_blank" rel="noopener noreferrer" class="title" data-title="Cell Surface Area Regulation and Membrane Tension">Cell Surface Area Regulation and Membrane Tension<span class="fa fa-external-link" aria-hidden="true"></span></a> <ul class="small references-list mb-0 mt-0" style="list-style-type:none; padding-left: 0; line-height:1.8em;"> <li class="authors">Morris, C. E.; Homann, U.</li> <li class="mb-0-5 source"> <span class="mr-1">The Journal of Membrane Biology, Vol. 179, Issue 2</span> <span class="text-muted"><a href="https://doi.org/10.1007/s002320010040" class="text-muted" target="_blank" rel="noopener noreferrer">https://doi.org/10.1007/s002320010040<span class="fa fa-external-link" aria-hidden="true"></span></a></span> </li> </ul> </td> <td class="small text-muted biblio-refs-td-secondary"> <span class="biblio-refs-type-badge ml-1 type" data-type="journal">journal</span> </td> <td class="small text-muted biblio-refs-td-secondary biblio-refs-td-secondary-date"><span class="date" data-date="2001-01-01">January 2001</span></td> </tr><tr> <td> <a href="https://doi.org/10.1007/BF00015814" target="_blank" rel="noopener noreferrer" class="title" data-title="Hybrid genes in the analysis of transformation conditions I Setting up a simple method for direct gene transfer in plant protoplasts">Hybrid genes in the analysis of transformation conditions: I. Setting up a simple method for direct gene transfer in plant protoplasts<span class="fa fa-external-link" aria-hidden="true"></span></a> <ul class="small references-list mb-0 mt-0" style="list-style-type:none; padding-left: 0; line-height:1.8em;"> <li class="authors">Negrutiu, I.; Shillito, R.; Potrykus, I.</li> <li class="mb-0-5 source"> <span class="mr-1">Plant Molecular Biology, Vol. 8, Issue 5</span> <span class="text-muted"><a href="https://doi.org/10.1007/BF00015814" class="text-muted" target="_blank" rel="noopener noreferrer">https://doi.org/10.1007/BF00015814<span class="fa fa-external-link" aria-hidden="true"></span></a></span> </li> </ul> </td> <td class="small text-muted biblio-refs-td-secondary"> <span class="biblio-refs-type-badge ml-1 type" data-type="journal">journal</span> </td> <td class="small text-muted biblio-refs-td-secondary biblio-refs-td-secondary-date"><span class="date" data-date="1987-01-01">January 1987</span></td> </tr><tr> <td> <a href="https://doi.org/10.1016/j.bbamem.2007.10.006" target="_blank" rel="noopener noreferrer" class="title" data-title="Laurdan fluorescence spectroscopy in the thylakoid bilayer The effect of violaxanthin to zeaxanthin conversion on the galactolipid dominated lipid environment">Laurdan fluorescence spectroscopy in the thylakoid bilayer: The effect of violaxanthin to zeaxanthin conversion on the galactolipid dominated lipid environment<span class="fa fa-external-link" aria-hidden="true"></span></a> <ul class="small references-list mb-0 mt-0" style="list-style-type:none; padding-left: 0; line-height:1.8em;"> <li class="authors">Szilágyi, Anna; Selstam, Eva; Åkerlund, Hans-Erik</li> <li class="mb-0-5 source"> <span class="mr-1">Biochimica et Biophysica Acta (BBA) - Biomembranes, Vol. 1778, Issue 1</span> <span class="text-muted"><a href="https://doi.org/10.1016/j.bbamem.2007.10.006" class="text-muted" target="_blank" rel="noopener noreferrer">https://doi.org/10.1016/j.bbamem.2007.10.006<span class="fa fa-external-link" aria-hidden="true"></span></a></span> </li> </ul> </td> <td class="small text-muted biblio-refs-td-secondary"> <span class="biblio-refs-type-badge ml-1 type" data-type="journal">journal</span> </td> <td class="small text-muted biblio-refs-td-secondary biblio-refs-td-secondary-date"><span class="date" data-date="2008-01-01">January 2008</span></td> </tr><tr> <td> <a href="https://doi.org/10.1002/aenm.201201014" target="_blank" rel="noopener noreferrer" class="title" data-title="Application of Nanoparticle Antioxidants to Enable Hyperstable Chloroplasts for Solar Energy Harvesting">Application of Nanoparticle Antioxidants to Enable Hyperstable Chloroplasts for Solar Energy Harvesting<span class="fa fa-external-link" aria-hidden="true"></span></a> <ul class="small references-list mb-0 mt-0" style="list-style-type:none; padding-left: 0; line-height:1.8em;"> <li class="authors">Boghossian, Ardemis A.; Sen, Fatih; Gibbons, Brenna M.</li> <li class="mb-0-5 source"> <span class="mr-1">Advanced Energy Materials, Vol. 3, Issue 7</span> <span class="text-muted"><a href="https://doi.org/10.1002/aenm.201201014" class="text-muted" target="_blank" rel="noopener noreferrer">https://doi.org/10.1002/aenm.201201014<span class="fa fa-external-link" aria-hidden="true"></span></a></span> </li> </ul> </td> <td class="small text-muted biblio-refs-td-secondary"> <span class="biblio-refs-type-badge ml-1 type" data-type="journal">journal</span> </td> <td class="small text-muted biblio-refs-td-secondary biblio-refs-td-secondary-date"><span class="date" data-date="2013-03-15">March 2013</span></td> </tr><tr> <td> <a href="https://doi.org/10.1007/BF00027310" target="_blank" rel="noopener noreferrer" class="title" data-title="Transient gene expression in electroporated Solanum protoplasts">Transient gene expression in electroporated Solanum protoplasts<span class="fa fa-external-link" aria-hidden="true"></span></a> <ul class="small references-list mb-0 mt-0" style="list-style-type:none; padding-left: 0; line-height:1.8em;"> <li class="authors">Jones, Heddwyn; Ooms, Gert; Jones, Michael G. K.</li> <li class="mb-0-5 source"> <span class="mr-1">Plant Molecular Biology, Vol. 13, Issue 5</span> <span class="text-muted"><a href="https://doi.org/10.1007/BF00027310" class="text-muted" target="_blank" rel="noopener noreferrer">https://doi.org/10.1007/BF00027310<span class="fa fa-external-link" aria-hidden="true"></span></a></span> </li> </ul> </td> <td class="small text-muted biblio-refs-td-secondary"> <span class="biblio-refs-type-badge ml-1 type" data-type="journal">journal</span> </td> <td class="small text-muted biblio-refs-td-secondary biblio-refs-td-secondary-date"><span class="date" data-date="1989-11-01">November 1989</span></td> </tr><tr> <td> <a href="https://doi.org/10.1038/nnano.2007.108" target="_blank" rel="noopener noreferrer" class="title" data-title="Mesoporous silica nanoparticles deliver DNA and chemicals into plants">Mesoporous silica nanoparticles deliver DNA and chemicals into plants<span class="fa fa-external-link" aria-hidden="true"></span></a> <ul class="small references-list mb-0 mt-0" style="list-style-type:none; padding-left: 0; line-height:1.8em;"> <li class="authors">Torney, François; Trewyn, Brian G.; Lin, Victor S. -Y.</li> <li class="mb-0-5 source"> <span class="mr-1">Nature Nanotechnology, Vol. 2, Issue 5</span> <span class="text-muted"><a href="https://doi.org/10.1038/nnano.2007.108" class="text-muted" target="_blank" rel="noopener noreferrer">https://doi.org/10.1038/nnano.2007.108<span class="fa fa-external-link" aria-hidden="true"></span></a></span> </li> </ul> </td> <td class="small text-muted biblio-refs-td-secondary"> <span class="biblio-refs-type-badge ml-1 type" data-type="journal">journal</span> </td> <td class="small text-muted biblio-refs-td-secondary biblio-refs-td-secondary-date"><span class="date" data-date="2007-04-29">April 2007</span></td> </tr><tr> <td> <a href="https://doi.org/10.1007/BF00388219" target="_blank" rel="noopener noreferrer" class="title" data-title="Surface charge of protoplasts and their significance in cellcell interaction">Surface charge of protoplasts and their significance in cell-cell interaction<span class="fa fa-external-link" aria-hidden="true"></span></a> <ul class="small references-list mb-0 mt-0" style="list-style-type:none; padding-left: 0; line-height:1.8em;"> <li class="authors">Nagata, Toshiyuki; Melchers, Georg</li> <li class="mb-0-5 source"> <span class="mr-1">Planta, Vol. 142, Issue 2</span> <span class="text-muted"><a href="https://doi.org/10.1007/BF00388219" class="text-muted" target="_blank" rel="noopener noreferrer">https://doi.org/10.1007/BF00388219<span class="fa fa-external-link" aria-hidden="true"></span></a></span> </li> </ul> </td> <td class="small text-muted biblio-refs-td-secondary"> <span class="biblio-refs-type-badge ml-1 type" data-type="journal">journal</span> </td> <td class="small text-muted biblio-refs-td-secondary biblio-refs-td-secondary-date"><span class="date" data-date="1978-01-01">January 1978</span></td> </tr><tr> <td> <a href="https://doi.org/10.1021/nn800325a" target="_blank" rel="noopener noreferrer" class="title" data-title="Carbon Nanotubes Protect DNA Strands during Cellular Delivery">Carbon Nanotubes Protect DNA Strands during Cellular Delivery<span class="fa fa-external-link" aria-hidden="true"></span></a> <ul class="small references-list mb-0 mt-0" style="list-style-type:none; padding-left: 0; line-height:1.8em;"> <li class="authors">Wu, Yanrong; Phillips, Joseph A.; Liu, Haipeng</li> <li class="mb-0-5 source"> <span class="mr-1">ACS Nano, Vol. 2, Issue 10</span> <span class="text-muted"><a href="https://doi.org/10.1021/nn800325a" class="text-muted" target="_blank" rel="noopener noreferrer">https://doi.org/10.1021/nn800325a<span class="fa fa-external-link" aria-hidden="true"></span></a></span> </li> </ul> </td> <td class="small text-muted biblio-refs-td-secondary"> <span class="biblio-refs-type-badge ml-1 type" data-type="journal">journal</span> </td> <td class="small text-muted biblio-refs-td-secondary biblio-refs-td-secondary-date"><span class="date" data-date="2008-10-08">October 2008</span></td> </tr><tr> <td> <a href="https://doi.org/10.17863/cam.7219" target="_blank" rel="noopener noreferrer" class="title" data-title="UreaHydroxyapatite Nanohybrids for Slow Release of Nitrogen">Urea-Hydroxyapatite Nanohybrids for Slow Release of Nitrogen<span class="fa fa-external-link" aria-hidden="true"></span></a> <ul class="small references-list mb-0 mt-0" style="list-style-type:none; padding-left: 0; line-height:1.8em;"> <li class="authors">Kottegoda, N.; Sandaruwan, C.; Priyadarshana, G.</li> <li class="mb-0-5 source"> <span class="mr-1">Apollo - University of Cambridge Repository</span> <span class="text-muted"><a href="https://doi.org/10.17863/cam.7219" class="text-muted" target="_blank" rel="noopener noreferrer">https://doi.org/10.17863/cam.7219<span class="fa fa-external-link" aria-hidden="true"></span></a></span> </li> </ul> </td> <td class="small text-muted biblio-refs-td-secondary"> <span class="biblio-refs-type-badge ml-1 type" data-type="text">text</span> </td> <td class="small text-muted biblio-refs-td-secondary biblio-refs-td-secondary-date"><span class="date" data-date="2017-01-01">January 2017</span></td> </tr><tr> <td> <a href="https://doi.org/10.2307/3868995" target="_blank" rel="noopener noreferrer" class="title" data-title="Molecular Biology of the Plasma Membrane of Higher Plants">Molecular Biology of the Plasma Membrane of Higher Plants<span class="fa fa-external-link" aria-hidden="true"></span></a> <ul class="small references-list mb-0 mt-0" style="list-style-type:none; padding-left: 0; line-height:1.8em;"> <li class="authors">Sussman, Michael R.; Harper, Jeffrey F.</li> <li class="mb-0-5 source"> <span class="mr-1">The Plant Cell, Vol. 1, Issue 10</span> <span class="text-muted"><a href="https://doi.org/10.2307/3868995" class="text-muted" target="_blank" rel="noopener noreferrer">https://doi.org/10.2307/3868995<span class="fa fa-external-link" aria-hidden="true"></span></a></span> </li> </ul> </td> <td class="small text-muted biblio-refs-td-secondary"> <span class="biblio-refs-type-badge ml-1 type" data-type="journal">journal</span> </td> <td class="small text-muted biblio-refs-td-secondary biblio-refs-td-secondary-date"><span class="date" data-date="1989-10-01">October 1989</span></td> </tr><tr> <td> <a href="https://doi.org/10.1002/ange.200460437" target="_blank" rel="noopener noreferrer" class="title" data-title="Functionalized Carbon Nanotubes for Plasmid DNA Gene Delivery">Functionalized Carbon Nanotubes for Plasmid DNA Gene Delivery<span class="fa fa-external-link" aria-hidden="true"></span></a> <ul class="small references-list mb-0 mt-0" style="list-style-type:none; padding-left: 0; line-height:1.8em;"> <li class="authors">Pantarotto, Davide; Singh, Ravi; McCarthy, David</li> <li class="mb-0-5 source"> <span class="mr-1">Angewandte Chemie, Vol. 116, Issue 39</span> <span class="text-muted"><a href="https://doi.org/10.1002/ange.200460437" class="text-muted" target="_blank" rel="noopener noreferrer">https://doi.org/10.1002/ange.200460437<span class="fa fa-external-link" aria-hidden="true"></span></a></span> </li> </ul> </td> <td class="small text-muted biblio-refs-td-secondary"> <span class="biblio-refs-type-badge ml-1 type" data-type="journal">journal</span> </td> <td class="small text-muted biblio-refs-td-secondary biblio-refs-td-secondary-date"><span class="date" data-date="2004-10-04">October 2004</span></td> </tr><tr> <td> <a href="https://doi.org/10.17615/2d36-8h90" target="_blank" rel="noopener noreferrer" class="title" data-title="Chemical Basis of Interactions Between Engineered Nanoparticles and Biological Systems">Chemical Basis of Interactions Between Engineered Nanoparticles and Biological Systems<span class="fa fa-external-link" aria-hidden="true"></span></a> <ul class="small references-list mb-0 mt-0" style="list-style-type:none; padding-left: 0; line-height:1.8em;"> <li class="authors">Qingxin, Mu,; Bing, Yan,; Hongyu, Zhou,</li> <li class="mb-0-5 source"> <span class="mr-1">The University of North Carolina at Chapel Hill University Libraries</span> <span class="text-muted"><a href="https://doi.org/10.17615/2d36-8h90" class="text-muted" target="_blank" rel="noopener noreferrer">https://doi.org/10.17615/2d36-8h90<span class="fa fa-external-link" aria-hidden="true"></span></a></span> </li> </ul> </td> <td class="small text-muted biblio-refs-td-secondary"> <span class="biblio-refs-type-badge ml-1 type" data-type="text">text</span> </td> <td class="small text-muted biblio-refs-td-secondary biblio-refs-td-secondary-date"><span class="date" data-date="2014-01-01">January 2014</span></td> </tr><tr> <td> <a href="https://doi.org/10.48550/arxiv.1202.2314" target="_blank" rel="noopener noreferrer" class="title" data-title="Surface patterning of carbon nanotubes can enhance their penetration through a phospholipid bilayer">Surface patterning of carbon nanotubes can enhance their penetration through a phospholipid bilayer<span class="fa fa-external-link" aria-hidden="true"></span></a> <ul class="small references-list mb-0 mt-0" style="list-style-type:none; padding-left: 0; line-height:1.8em;"> <li class="authors">Pogodin, Sergey; Slater, Nigel K. H.; Baulin, Vladimir A.</li> <li class="mb-0-5 source"> <span class="mr-1">arXiv</span> <span class="text-muted"><a href="https://doi.org/10.48550/arxiv.1202.2314" class="text-muted" target="_blank" rel="noopener noreferrer">https://doi.org/10.48550/arxiv.1202.2314<span class="fa fa-external-link" aria-hidden="true"></span></a></span> </li> </ul> </td> <td class="small text-muted biblio-refs-td-secondary"> <span class="biblio-refs-type-badge ml-1 type" data-type="text">text</span> </td> <td class="small text-muted biblio-refs-td-secondary biblio-refs-td-secondary-date"><span class="date" data-date="2012-01-01">January 2012</span></td> </tr></tbody></table> </div> </div> </section> <hr class="mt-2 mb-2"/> <section id="section-citations" data-list="list_cite" class="tab-content-sec osti-curated biblio-section section-scroll" style="scroll-margin-top:110px;"> <div class="biblio-section-navbar active row"> <div class="col"> <h2 class="abstract biblio-section-title mt-0 mb-0-5" style="cursor:pointer;"><span class="fa indicator"></span> Cited By (4) <span class="text-muted list-table-filternum"></span></h2> </div> <div class="col-md-12 col-lg-auto"> <form class="pure-form reference-filter d-print-none"> <div class="small biblio-section-navbar-tool"> <label for="list-table-search-citations" class="sr-only">Search citations: </label> <input type="text" class="list-table-search mt-0 mb-0-5 ml-1" id="list-table-search-citations" placeholder="Search citations ..."> </div> <div class="small biblio-section-navbar-tool"> <select class="list-table-sort mt-0 mb-0-5"> <option value="date">Sort by Date</option> <option value="title">Sort by Title</option> </select> </div> <div class="small biblio-section-navbar-tool"> <button type="reset" class="pure-button list-table-reset mt-0 mb-0-5">↺</button> </div> </form> </div> </div> <div class="row"> <div class="col-sm-12"> <table id="list-table-citations" class="truncate-rows list-table table-striped" data-rows="5" style="width:100%;"><tbody class="list"><tr> <td> <a href="https://doi.org/10.1002/admt.201900657" target="_blank" rel="noopener noreferrer" class="title" data-title="The Emergence of Plant Nanobionics and Living Plants as Technology">The Emergence of Plant Nanobionics and Living Plants as Technology<span class="fa fa-external-link" aria-hidden="true"></span></a> <ul class="small references-list mb-0 mt-0" style="list-style-type:none; padding-left: 0; line-height:1.8em;"> <li class="authors">Lew, Tedrick Thomas Salim; Koman, Volodymyr B.; Gordiichuk, Pavlo</li> <li class="mb-0-5 source"> <span class="mr-1">Advanced Materials Technologies, Vol. 5, Issue 3</span> <span class="text-muted"><a href="https://doi.org/10.1002/admt.201900657" class="text-muted" target="_blank" rel="noopener noreferrer">https://doi.org/10.1002/admt.201900657<span class="fa fa-external-link" aria-hidden="true"></span></a></span> </li> </ul> </td> <td class="small text-muted biblio-refs-td-secondary"> <span class="biblio-refs-type-badge ml-1 type" data-type="journal">journal</span> </td> <td class="small text-muted biblio-refs-td-secondary biblio-refs-td-secondary-date"><span class="date" data-date="2019-11-08">November 2019</span></td> </tr><tr> <td> <a href="https://doi.org/10.1007/s12274-019-2438-0" target="_blank" rel="noopener noreferrer" class="title" data-title="Enhancing bioelectricity generation in microbial fuel cells and biophotovoltaics using nanomaterials">Enhancing bioelectricity generation in microbial fuel cells and biophotovoltaics using nanomaterials<span class="fa fa-external-link" aria-hidden="true"></span></a> <ul class="small references-list mb-0 mt-0" style="list-style-type:none; padding-left: 0; line-height:1.8em;"> <li class="authors">Mouhib, Mohammed; Antonucci, Alessandra; Reggente, Melania</li> <li class="mb-0-5 source"> <span class="mr-1">Nano Research, Vol. 12, Issue 9</span> <span class="text-muted"><a href="https://doi.org/10.1007/s12274-019-2438-0" class="text-muted" target="_blank" rel="noopener noreferrer">https://doi.org/10.1007/s12274-019-2438-0<span class="fa fa-external-link" aria-hidden="true"></span></a></span> </li> </ul> </td> <td class="small text-muted biblio-refs-td-secondary"> <span class="biblio-refs-type-badge ml-1 type" data-type="journal">journal</span> </td> <td class="small text-muted biblio-refs-td-secondary biblio-refs-td-secondary-date"><span class="date" data-date="2019-06-11">June 2019</span></td> </tr><tr> <td> <a href="https://doi.org/10.1038/s41565-019-0375-4" target="_blank" rel="noopener noreferrer" class="title" data-title="Chloroplastselective gene delivery and expression in planta using chitosancomplexed singlewalled carbon nanotube carriers">Chloroplast-selective gene delivery and expression in planta using chitosan-complexed single-walled carbon nanotube carriers<span class="fa fa-external-link" aria-hidden="true"></span></a> <ul class="small references-list mb-0 mt-0" style="list-style-type:none; padding-left: 0; line-height:1.8em;"> <li class="authors">Kwak, Seon-Yeong; Lew, Tedrick Thomas Salim; Sweeney, Connor J.</li> <li class="mb-0-5 source"> <span class="mr-1">Nature Nanotechnology, Vol. 14, Issue 5</span> <span class="text-muted"><a href="https://doi.org/10.1038/s41565-019-0375-4" class="text-muted" target="_blank" rel="noopener noreferrer">https://doi.org/10.1038/s41565-019-0375-4<span class="fa fa-external-link" aria-hidden="true"></span></a></span> </li> </ul> </td> <td class="small text-muted biblio-refs-td-secondary"> <span class="biblio-refs-type-badge ml-1 type" data-type="journal">journal</span> </td> <td class="small text-muted biblio-refs-td-secondary biblio-refs-td-secondary-date"><span class="date" data-date="2019-02-25">February 2019</span></td> </tr><tr> <td> <a href="https://doi.org/10.1038/s41565-019-0382-5" target="_blank" rel="noopener noreferrer" class="title" data-title="High aspect ratio nanomaterials enable delivery of functional genetic material without DNA integration in mature plants">High aspect ratio nanomaterials enable delivery of functional genetic material without DNA integration in mature plants<span class="fa fa-external-link" aria-hidden="true"></span></a> <ul class="small references-list mb-0 mt-0" style="list-style-type:none; padding-left: 0; line-height:1.8em;"> <li class="authors">Demirer, Gozde S.; Zhang, Huan; Matos, Juliana L.</li> <li class="mb-0-5 source"> <span class="mr-1">Nature Nanotechnology, Vol. 14, Issue 5</span> <span class="text-muted"><a href="https://doi.org/10.1038/s41565-019-0382-5" class="text-muted" target="_blank" rel="noopener noreferrer">https://doi.org/10.1038/s41565-019-0382-5<span class="fa fa-external-link" aria-hidden="true"></span></a></span> </li> </ul> </td> <td class="small text-muted biblio-refs-td-secondary"> <span class="biblio-refs-type-badge ml-1 type" data-type="journal">journal</span> </td> <td class="small text-muted biblio-refs-td-secondary biblio-refs-td-secondary-date"><span class="date" data-date="2019-02-25">February 2019</span></td> </tr></tbody></table> </div> </div> </section> <hr class="mt-2 mb-2"/> <section id="section-similar" class="tab-content-sec osti-curated biblio-section section-scroll" style="scroll-margin-top:110px;"> <div class="row"> <div class="col-8" style="padding-right:40px;"> <h2 class="abstract">Similar Records</h2> <div class="" style="line-height:1.6rem;"> <a href="/biblio/1506633" target="_blank">Effect of CeO<sub>2</sub> nanomaterial surface functional groups on tissue and subcellular distribution of Ce in tomato (<em>Solanum lycopersicum</em>)</a> <div class="small" style="line-height:1.4rem;"> Journal Article <span style="padding-left: 0.75rem;padding-right: 0.75rem;">·</span> Mon Dec 10 00:00:00 EST 2018 <span style="padding-left: 0.75rem;padding-right: 0.75rem;">·</span> Environmental Science: Nano <span style="padding-left: 0.75rem;padding-right: 0.75rem;">·</span> <span class="text-muted" style="padding-right:0.5em;">OSTI ID:</span>1609981 </div> <div class="small" style="line-height:1.4rem;"> <span class="author-link"><a href="/search/author:"Li, Jieran"" class="btn btn-link btn-sm"><span class="author" itemprop="author">Li, Jieran; </span></a></span> <span class="author-link"><a href="/search/author:"Tappero, Ryan V."" class="btn btn-link btn-sm"><span class="author" itemprop="author">Tappero, Ryan V.; </span></a></span> <span class="author-link"><a href="/search/author:"Acerbo, Alvin S."" class="btn btn-link btn-sm"><span class="author" itemprop="author">Acerbo, Alvin S.; </span></a></span> <span class="text-muted">+4 more</span> </div> <hr/> <a href="/biblio/1836619" target="_blank">Carbon nanotube biocompatibility in plants is determined by their surface chemistry</a> <div class="small" style="line-height:1.4rem;"> Journal Article <span style="padding-left: 0.75rem;padding-right: 0.75rem;">·</span> Mon Dec 20 00:00:00 EST 2021 <span style="padding-left: 0.75rem;padding-right: 0.75rem;">·</span> Journal of Nanobiotechnology <span style="padding-left: 0.75rem;padding-right: 0.75rem;">·</span> <span class="text-muted" style="padding-right:0.5em;">OSTI ID:</span>1609981 </div> <div class="small" style="line-height:1.4rem;"> <span class="author-link"><a href="/search/author:"González-Grandío, Eduardo"" class="btn btn-link btn-sm"><span class="author" itemprop="author">González-Grandío, Eduardo; </span></a></span> <span class="author-link"><a href="/search/author:"Demirer, Gözde S."" class="btn btn-link btn-sm"><span class="author" itemprop="author">Demirer, Gözde S.; </span></a></span> <span class="author-link"><a href="/search/author:"Jackson, Christopher T."" class="btn btn-link btn-sm"><span class="author" itemprop="author">Jackson, Christopher T.; </span></a></span> <span class="text-muted">+5 more</span> </div> <hr/> <a href="/biblio/1643174" target="_blank">Lights up on organelles: Optogenetic tools to control subcellular structure and organization</a> <div class="small" style="line-height:1.4rem;"> Journal Article <span style="padding-left: 0.75rem;padding-right: 0.75rem;">·</span> Sun Jul 26 00:00:00 EDT 2020 <span style="padding-left: 0.75rem;padding-right: 0.75rem;">·</span> WIREs Mechanisms of Disease <span style="padding-left: 0.75rem;padding-right: 0.75rem;">·</span> <span class="text-muted" style="padding-right:0.5em;">OSTI ID:</span>1609981 </div> <div class="small" style="line-height:1.4rem;"> <span class="author-link"><a href="/search/author:"Kichuk, Therese C."" class="btn btn-link btn-sm"><span class="author" itemprop="author">Kichuk, Therese C.; </span></a></span> <span class="author-link"><a href="/search/author:"Carrasco‐López, César"" class="btn btn-link btn-sm"><span class="author" itemprop="author">Carrasco‐López, César; </span></a></span> <span class="author-link"><a href="/search/author:"Avalos, José L."" class="btn btn-link btn-sm"><span class="author" itemprop="author">Avalos, José L.</span></a></span> </div> </div> </div> <div class="col-4" style="padding-left:40px; border-left:1px solid #ddd;"> <h2 class="abstract">Related Subjects</h2> <div class="small" style="line-height:1.6rem;"> <a href="/search/subject:36 MATERIALS SCIENCE">36 MATERIALS SCIENCE</a><br/> <a href="/search/subject:Chemistry">Chemistry</a><br/> <a href="/search/subject:Science Technology">Science & Technology</a><br/> <a href="/search/subject:Materials Science">Materials Science</a><br/> <a href="/search/subject:Physics">Physics</a><br/> </div> </div> </div> </section> </div> </section> <footer class="" style="background-color:#f9f9f9; /* padding-top: 0.5rem; */"> <div class="footer-minor"> <div class="container"> <hr class="footer-separator" /> <div class="text-center" style="margin-top:1.25rem;"> <div class="pure-menu pure-menu-horizontal"> <ul class="pure-menu-list" id="footer-org-menu"> <li class="pure-menu-item d-block d-sm-inline-block"> <a href="https://energy.gov" target="_blank" rel="noopener noreferrer"> <img src="data:image/gif;base64,R0lGODlhAQABAIAAAP///wAAACH5BAEAAAAALAAAAAABAAEAAAICRAEAOw==" class="sprite sprite-footer-us-doe-min" alt="U.S. Department of Energy" /> </a> </li> <li class="pure-menu-item d-block d-sm-inline-block"> <a href="https://www.energy.gov/science/office-science" target="_blank" rel="noopener noreferrer"> <img src="data:image/gif;base64,R0lGODlhAQABAIAAAP///wAAACH5BAEAAAAALAAAAAABAAEAAAICRAEAOw==" class="sprite sprite-footer-office-of-science-min" alt="Office of Science" /> </a> </li> <li class="pure-menu-item d-block d-sm-inline-block"> <a href="/"> <img src="data:image/gif;base64,R0lGODlhAQABAIAAAP///wAAACH5BAEAAAAALAAAAAABAAEAAAICRAEAOw==" class="sprite sprite-footer-osti-min" alt="Office of Scientific and Technical Information" /> </a> </li> </ul> </div> </div> <div class="text-center small" style="margin-top:0.5em;margin-bottom:2.0rem;"> <div class="pure-menu pure-menu-horizontal"> <ul class="pure-menu-list"> <li class="pure-menu-item"><a href="/disclaim" class="pure-menu-link"><span class="fa fa-institution"></span> Website Policies <span class="d-none d-sm-inline" style="color:#737373;">/ Important Links</span></a></li> <li class="pure-menu-item"><a href="/contact" class="pure-menu-link"><span class="fa fa-comments-o"></span> Contact Us</a></li> <li class="d-block d-md-none mb-1"></li> <li class="pure-menu-item"><a href="https://doe.responsibledisclosure.com/hc/en-us" target="_blank" class="pure-menu-link">Vulnerability Disclosure Program</a></li> <li class="d-block d-lg-none mb-1"></li> <li class="pure-menu-item"><a href="https://www.facebook.com/ostigov" target="_blank" rel="noopener noreferrer" class="pure-menu-link social"><span class="fa fa-facebook"></span><span class="sr-only">Facebook</span></a></li> <li class="pure-menu-item"><a href="https://twitter.com/OSTIgov" target="_blank" rel="noopener noreferrer" class="pure-menu-link social"><span class="fa fa-twitter"></span><span class="sr-only">Twitter</span></a></li> <li class="pure-menu-item"><a href="https://www.youtube.com/user/ostigov" target="_blank" rel="noopener noreferrer" class="pure-menu-link social"><span class="fa fa-youtube-play"></span><span class="sr-only">YouTube</span></a></li> </ul> </div> </div> </div> </div> </footer> <link href="/css/ostigov.fonts.240417.1512.css" rel="stylesheet"> <script src="/js/ostigov.240417.1512.js"></script><noscript></noscript> <script defer src="/js/ostigov.biblio.240417.1512.js"></script><noscript></noscript> <script async type="text/javascript" src="/js/Universal-Federated-Analytics-Min.js?agency=DOE" id="_fed_an_ua_tag"></script><noscript></noscript> </body> <!-- OSTI.GOV v.240417.1512 --> </html>

Title: Rational Design Principles for the Transport and Subcellular Distribution of Nanomaterials into Plant Protoplasts

Citation Formats

References (87)