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Title: Molecular Factors of Hypochlorite Tolerance in the Hypersaline Archaeon Haloferax volcanii

Abstract

Halophilic archaea thrive in hypersaline conditions associated with desiccation, ultraviolet (UV) irradiation and redox active compounds, and thus are naturally tolerant to a variety of stresses. Here, we identified mutations that promote enhanced tolerance of halophilic archaea to redox-active compounds using Haloferax volcanii as a model organism. The strains were isolated from a library of random transposon mutants for growth on high doses of sodium hypochlorite (NaOCl), an agent that forms hypochlorous acid (HOCl) and other redox acid compounds common to aqueous environments of high concentrations of chloride. The transposon insertion site in each of twenty isolated clones was mapped using the following: (i) inverse nested two-step PCR (INT-PCR) and (ii) semi-random two-step PCR (ST-PCR). Genes that were found to be disrupted in hypertolerant strains were associated with lysine deacetylation, proteasomes, transporters, polyamine biosynthesis, electron transfer, and other cellular processes. Further analysis revealed a ΔpsmA1 (α1) markerless deletion strain that produces only the α2 and β proteins of 20S proteasomes was hypertolerant to hypochlorite stress compared with wild type, which produces α1, α2, and β proteins. The results of this study provide new insights into archaeal tolerance of redox active compounds such as hypochlorite.

Authors:
 [1];  [1];  [1];  [1];  [1];  [1];  [1]; ORCiD logo [2];  [3]; ORCiD logo [1]
  1. Univ. of Florida, Gainesville, FL (United States)
  2. Univ. of Helsinki (Finland)
  3. Univ. of Turku (Finland)
Publication Date:
Research Org.:
Univ. of Florida, Gainesville, FL (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Basic Energy Sciences (BES) (SC-22). Chemical Sciences, Geosciences & Biosciences Division; National Institutes of Health (NIH)
OSTI Identifier:
1609594
Grant/Contract Number:  
FG02-05ER15650
Resource Type:
Journal Article: Accepted Manuscript
Journal Name:
Genes
Additional Journal Information:
Journal Volume: 9; Journal Issue: 11; Journal ID: ISSN 2073-4425
Publisher:
MDPI
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; Genetics & Heredity; archaea; oxidative stress; hypochlorite; redox-active; proteasome

Citation Formats

Gomez, Miguel, Leung, Whinkie, Dantuluri, Swathi, Pillai, Alexander, Gani, Zyan, Hwang, Sungmin, McMillan, Lana J., Kiljunen, Saija, Savilahti, Harri, and Maupin-Furlow, Julie A. Molecular Factors of Hypochlorite Tolerance in the Hypersaline Archaeon Haloferax volcanii. United States: N. p., 2018. Web. doi:10.3390/genes9110562.
Gomez, Miguel, Leung, Whinkie, Dantuluri, Swathi, Pillai, Alexander, Gani, Zyan, Hwang, Sungmin, McMillan, Lana J., Kiljunen, Saija, Savilahti, Harri, & Maupin-Furlow, Julie A. Molecular Factors of Hypochlorite Tolerance in the Hypersaline Archaeon Haloferax volcanii. United States. doi:10.3390/genes9110562.
Gomez, Miguel, Leung, Whinkie, Dantuluri, Swathi, Pillai, Alexander, Gani, Zyan, Hwang, Sungmin, McMillan, Lana J., Kiljunen, Saija, Savilahti, Harri, and Maupin-Furlow, Julie A. Tue . "Molecular Factors of Hypochlorite Tolerance in the Hypersaline Archaeon Haloferax volcanii". United States. doi:10.3390/genes9110562. https://www.osti.gov/servlets/purl/1609594.
@article{osti_1609594,
title = {Molecular Factors of Hypochlorite Tolerance in the Hypersaline Archaeon Haloferax volcanii},
author = {Gomez, Miguel and Leung, Whinkie and Dantuluri, Swathi and Pillai, Alexander and Gani, Zyan and Hwang, Sungmin and McMillan, Lana J. and Kiljunen, Saija and Savilahti, Harri and Maupin-Furlow, Julie A.},
abstractNote = {Halophilic archaea thrive in hypersaline conditions associated with desiccation, ultraviolet (UV) irradiation and redox active compounds, and thus are naturally tolerant to a variety of stresses. Here, we identified mutations that promote enhanced tolerance of halophilic archaea to redox-active compounds using Haloferax volcanii as a model organism. The strains were isolated from a library of random transposon mutants for growth on high doses of sodium hypochlorite (NaOCl), an agent that forms hypochlorous acid (HOCl) and other redox acid compounds common to aqueous environments of high concentrations of chloride. The transposon insertion site in each of twenty isolated clones was mapped using the following: (i) inverse nested two-step PCR (INT-PCR) and (ii) semi-random two-step PCR (ST-PCR). Genes that were found to be disrupted in hypertolerant strains were associated with lysine deacetylation, proteasomes, transporters, polyamine biosynthesis, electron transfer, and other cellular processes. Further analysis revealed a ΔpsmA1 (α1) markerless deletion strain that produces only the α2 and β proteins of 20S proteasomes was hypertolerant to hypochlorite stress compared with wild type, which produces α1, α2, and β proteins. The results of this study provide new insights into archaeal tolerance of redox active compounds such as hypochlorite.},
doi = {10.3390/genes9110562},
journal = {Genes},
issn = {2073-4425},
number = 11,
volume = 9,
place = {United States},
year = {2018},
month = {11}
}

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