Molecular Factors of Hypochlorite Tolerance in the Hypersaline Archaeon Haloferax volcanii

Gomez, Miguel; Leung, Whinkie; Dantuluri, Swathi; Pillai, Alexander; Gani, Zyan; Hwang, Sungmin; McMillan, Lana J.; Kiljunen, Saija; Savilahti, Harri; Maupin-Furlow, Julie A.

doi:10.3390/genes9110562

Title: Molecular Factors of Hypochlorite Tolerance in the Hypersaline Archaeon Haloferax volcanii

Journal Article · Tue Nov 20 00:00:00 EST 2018 · Genes

DOI:https://doi.org/10.3390/genes9110562· OSTI ID:1609594

Gomez, Miguel ^[1]; Leung, Whinkie ^[1]; Dantuluri, Swathi ^[1]; Pillai, Alexander ^[1]; Gani, Zyan ^[1]; Hwang, Sungmin ^[1]; McMillan, Lana J. ^[1];

^[2]; Savilahti, Harri ^[3];

^[1]

Univ. of Florida, Gainesville, FL (United States)
Univ. of Helsinki (Finland)
Univ. of Turku (Finland)

Halophilic archaea thrive in hypersaline conditions associated with desiccation, ultraviolet (UV) irradiation and redox active compounds, and thus are naturally tolerant to a variety of stresses. Here, we identified mutations that promote enhanced tolerance of halophilic archaea to redox-active compounds using Haloferax volcanii as a model organism. The strains were isolated from a library of random transposon mutants for growth on high doses of sodium hypochlorite (NaOCl), an agent that forms hypochlorous acid (HOCl) and other redox acid compounds common to aqueous environments of high concentrations of chloride. The transposon insertion site in each of twenty isolated clones was mapped using the following: (i) inverse nested two-step PCR (INT-PCR) and (ii) semi-random two-step PCR (ST-PCR). Genes that were found to be disrupted in hypertolerant strains were associated with lysine deacetylation, proteasomes, transporters, polyamine biosynthesis, electron transfer, and other cellular processes. Further analysis revealed a ΔpsmA1 (α1) markerless deletion strain that produces only the α2 and β proteins of 20S proteasomes was hypertolerant to hypochlorite stress compared with wild type, which produces α1, α2, and β proteins. The results of this study provide new insights into archaeal tolerance of redox active compounds such as hypochlorite.

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Research Organization:: Univ. of Florida, Gainesville, FL (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Basic Energy Sciences (BES). Chemical Sciences, Geosciences, and Biosciences Division; National Institutes of Health (NIH)

Grant/Contract Number:: FG02-05ER15650

OSTI ID:: 1609594

Journal Information:: Genes, Vol. 9, Issue 11; ISSN 2073-4425

Publisher:: MDPICopyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 5 works

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59 BASIC BIOLOGICAL SCIENCES
Genetics & Heredity
archaea
oxidative stress
hypochlorite
redox-active
proteasome

Title: Molecular Factors of Hypochlorite Tolerance in the Hypersaline Archaeon Haloferax volcanii

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