Rhodanese-Like Domain Protein UbaC and Its Role in Ubiquitin-Like Protein Modification and Sulfur Mobilization in Archaea

Hepowit, Nathaniel L.; Maupin-Furlow, Julie A.

doi:10.1128/jb.00254-19

Rhodanese-Like Domain Protein UbaC and Its Role in Ubiquitin-Like Protein Modification and Sulfur Mobilization in Archaea

Journal Article · Wed Jul 10 04:00:00 EDT 2019 · Journal of Bacteriology

DOI:https://doi.org/10.1128/jb.00254-19· OSTI ID:1609593

Hepowit, Nathaniel L. ^[1]; Maupin-Furlow, Julie A. ^[2]

Univ. of Florida, Gainesville, FL (United States); DOE/OSTI
Univ. of Florida, Gainesville, FL (United States)

Ubiquitin-like protein (Ubl) modification targets proteins for transient inactivation and/or proteasome-mediated degradation in archaea. In this work the rhodanese-like domain (RHD) protein UbaC was found to copurify with the E1-like enzyme (UbaA) of the Ubl modification machinery in the archaeonHaloferax volcanii. UbaC was shown to be important for Ubl ligation, particularly for the attachment of the Ubl SAMP2/3s to protein targets after exposure to oxidants (NaOCl, dimethyl sulfoxide [DMSO], and methionine sulfoxide [MetO]) and the proteasome inhibitor bortezomib. While UbaC was needed for ligation of the Ubl SAMP1 to MoaE (the large subunit of molybdopterin synthase), it was not important in the formation of oxidant-induced SAMP1 protein conjugates. Indicative of defects in sulfur relay, mutation ofubaCimpaired molybdenum cofactor (Moco)-dependent DMSO reductase activity and cell survival at elevated temperature, suggesting a correlation with defects in the 2-thiolated state of wobble uridine tRNA. Overall, the archaeal stand-alone RHD UbaC has an important function in Ubl ligation and is associated with sulfur relay processes.

View Accepted Manuscript (DOE)

Research Organization:: Univ. of Florida, Gainesville, FL (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Basic Energy Sciences (BES) (SC-22). Chemical Sciences, Geosciences & Biosciences Division; National Institutes of Health (NIH)

Grant/Contract Number:: FG02-05ER15650

OSTI ID:: 1609593

Journal Information:: Journal of Bacteriology, Journal Name: Journal of Bacteriology Journal Issue: 15 Vol. 201; ISSN 0021-9193

Publisher:: American Society for MicrobiologyCopyright Statement

Country of Publication:: United States

Language:: English

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Rhodanese-Like Domain Protein UbaC and Its Role in Ubiquitin-Like Protein Modification and Sulfur Mobilization in Archaea

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