A predicted plastid rhomboid protease affects phosphatidic acid metabolism in Arabidopsis thaliana

Lavell, Anastasiya; Froehlich, John E.; Baylis, Olivia; Rotondo, Anthony D.; Benning, Christoph

doi:10.1111/tpj.14377

A predicted plastid rhomboid protease affects phosphatidic acid metabolism in Arabidopsis thaliana

Journal Article · Tue May 07 00:00:00 EDT 2019 · The Plant Journal

DOI:https://doi.org/10.1111/tpj.14377· OSTI ID:1608419

Lavell, Anastasiya ^[1]; Froehlich, John E. ^[2]; Baylis, Olivia ^[2]; Rotondo, Anthony D. ^[2]; ^[2]

Michigan State Univ., East Lansing, MI (United States). MSU-DOE Plant Research Laboratory; MSU DOE Plant Research Laboratory
Michigan State Univ., East Lansing, MI (United States). MSU-DOE Plant Research Laboratory

The thylakoid membranes of the chloroplast harbor the photosynthetic machinery that converts light into chemical energy. Chloroplast membranes are unique in their lipid makeup, which is dominated by the galactolipids mono- and digalactosyldiacylglycerol (MGDG and DGDG). Additionally, the most abundant galactolipid, MGDG, is assembled through both plastid and endoplasmic reticulum (ER) pathways in Arabidopsis, resulting in distinguishable molecular lipid species. Phosphatidic acid (PA) is the first glycerolipid formed by the plastid galactolipid biosynthetic pathway. It is converted to substrate diacylglycerol (DAG) for MGDG Synthase (MGD1) which adds to it a galactose from UDP-Gal. The enzymatic reactions yielding these galactolipids have been well established. However, auxiliary or regulatory factors are largely unknown. We identified a predicted rhomboid-like protease 10 (RBL10), located in plastids of Arabidopsis thaliana, that affects galactolipid biosynthesis likely through intramembrane proteolysis. Plants with T-DNA disruptions in RBL10 have greatly decreased 16:3 (acyl carbons:double bonds) and increased 18:3 acyl chain abundance in MGDG of leaves. Additionally, rbl10-1 mutants show reduced [¹⁴C]-acetate incorporation into MGDG during pulse-chase labeling, indicating a reduced flux through the plastid galactolipid biosynthesis pathway. While plastid MGDG biosynthesis is blocked in rbl10-1 mutants, they are capable of synthesizing PA, as well as producing normal amounts of MGDG by compensating with ER-derived lipid precursors. In conclusion, these findings link this predicted protease to the utilization of PA for plastid galactolipid biosynthesis potentially revealing a regulatory mechanism in chloroplasts.

View Accepted Manuscript (DOE)

Research Organization:: Michigan State Univ., East Lansing, MI (United States). MSU-DOE Plant Research Laboratory

Sponsoring Organization:: USDOE Office of Science (SC), Basic Energy Sciences (BES) (SC-22). Chemical Sciences, Geosciences & Biosciences Division; National Institutes of Health (NIH)

Grant/Contract Number:: FG02-91ER20021

OSTI ID:: 1608419

Journal Information:: The Plant Journal, Journal Name: The Plant Journal Journal Issue: 5 Vol. 99; ISSN 0960-7412

Publisher:: Society for Experimental BiologyCopyright Statement

Country of Publication:: United States

Language:: English

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A predicted plastid rhomboid protease affects phosphatidic acid metabolism in Arabidopsis thaliana

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References (50)

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