A DNA G-quadruplex/i-motif hybrid

Chu, Betty; Zhang, Daoning; Paukstelis, Paul J.

doi:10.1093/nar/gkz1008

Title: A DNA G-quadruplex/i-motif hybrid

Journal Article · Thu Nov 14 00:00:00 EST 2019 · Nucleic Acids Research

DOI:https://doi.org/10.1093/nar/gkz1008· OSTI ID:1607551

Chu, Betty ^[1]; Zhang, Daoning ^[1]; Paukstelis, Paul J. ^[1]

Department of Chemistry and Biochemistry, Center for Biomolecular Structure and Organization, University of Maryland, College Park, MD 20742, USA

Abstract DNA can form many structures beyond the canonical Watson–Crick double helix. It is now clear that noncanonical structures are present in genomic DNA and have biological functions. G-rich G-quadruplexes and C-rich i-motifs are the most well-characterized noncanonical DNA motifs that have been detected in vivo with either proscribed or postulated biological roles. Because of their independent sequence requirements, these structures have largely been considered distinct types of quadruplexes. Here, we describe the crystal structure of the DNA oligonucleotide, d(CCAGGCTGCAA), that self-associates to form a quadruplex structure containing two central antiparallel G-tetrads and six i-motif C–C+ base pairs. Solution studies suggest a robust structural motif capable of assembling as a tetramer of individual strands or as a dimer when composed of tandem repeats. This hybrid structure highlights the growing structural diversity of DNA and suggests that biological systems may harbor many functionally important non-duplex structures.

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Research Organization:: Argonne National Laboratory (ANL), Argonne, IL (United States). Advanced Photon Source (APS)

Sponsoring Organization:: USDOE Office of Science (SC); National Institutes of Health (NIH)

Grant/Contract Number:: AC02-05CH11231; P30 GM124165; S10RR25528; S10RR02897

OSTI ID:: 1607551

Alternate ID(s):: OSTI ID: 1578242

Journal Information:: Nucleic Acids Research, Journal Name: Nucleic Acids Research; ISSN 0305-1048

Publisher:: Oxford University PressCopyright Statement

Country of Publication:: United Kingdom

Language:: English

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Cited by: 16 works

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