NanoSIMS: Microscale Quantification of Biogeochemical Activity with Large-Scale Impacts

Mayali, Xavier

doi:10.1146/annurev-marine-010419-010714

NanoSIMS: Microscale Quantification of Biogeochemical Activity with Large-Scale Impacts

Journal Article · Thu Jan 02 23:00:00 EST 2020 · Annual Review of Marine Science

DOI:https://doi.org/10.1146/annurev-marine-010419-010714· OSTI ID:1606479

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Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)

One major objective of aquatic microbial ecology is to understand the distribution of microbial populations over space and time and in response to environmental factors. Perhaps more importantly, it is crucial to quantify how those microbial cells affect biogeochemical processes of interest, such as primary production, nitrogen cycling, or the breakdown of pollutants. One valuable approach to link microbial identity to activity is to carry out incubations with stable-isotope-labeled substrates and then quantify the isotope incorporation by individual microbial cells using nanoscale secondary ion mass spectrometry (NanoSIMS). This review summarizes recent efforts in this field, highlights novel methods, describes studies investigating rare metabolisms as well as widespread microbial activity, and hopes to provide a framework to increase the use and capabilities of NanoSIMS for microbial biogeochemical studies in the future.

View Accepted Manuscript (DOE)

Research Organization:: Lawrence Livermore National Laboratory (LLNL), Livermore, CA (United States)

Sponsoring Organization:: USDOE National Nuclear Security Administration (NNSA)

Grant/Contract Number:: AC52-07NA27344

OSTI ID:: 1606479

Report Number(s):: LLNL-JRNL--771853; 963320

Journal Information:: Annual Review of Marine Science, Journal Name: Annual Review of Marine Science Journal Issue: 1 Vol. 12; ISSN 1941-1405

Publisher:: Annual ReviewsCopyright Statement

Country of Publication:: United States

Language:: English

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