Interprotein electron transfer biohybrid system for photocatalytic H2 production

Brahmachari, Udita; Pokkuluri, P. Raj; Tiede, David M.; Niklas, Jens; Poluektov, Oleg G.; Mulfort, Karen L.; Utschig, Lisa M.

doi:10.1007/s11120-019-00705-x

Interprotein electron transfer biohybrid system for photocatalytic H₂ production

Journal Article · Fri Jan 10 00:00:00 EST 2020 · Photosynthesis Research

DOI:https://doi.org/10.1007/s11120-019-00705-x· OSTI ID:1606243

Brahmachari, Udita ^[1]; Pokkuluri, P. Raj ^[1]; Tiede, David M. ^[1]; Niklas, Jens ^[1]; Poluektov, Oleg G. ^[1]; Mulfort, Karen L. ^[1]; Utschig, Lisa M. ^[1]

Argonne National Lab. (ANL), Argonne, IL (United States)

Around the world there is a large research investment in developing solar fuel systems as clean and sustainable sources of energy. The fundamental mechanisms of natural photosynthesis can provide a source of inspiration for these studies. Photosynthetic reaction center (RC) proteins capture and convert light energy into chemical energy that is ultimately used to drive oxygenic water-splitting and carbon fixation. For the light energy to be used, the RC communicates with other donor/acceptor components via a sophisticated electron transfer scheme that includes electron transfer reactions between soluble and membrane bound proteins. Herein, we reengineer an inherent interprotein electron transfer pathway in a natural photosynthetic system to make it photocatalytic for aqueous H₂ production. The native electron shuttle protein ferredoxin (Fd) is used as a scaffold for binding of a ruthenium photosensitizer and H₂ catalytic function is imparted to its partner protein, ferredoxin-NADP⁺-reductase (FNR), by attachment of cobaloxime molecules. We find that this 2-protein biohybrid system produces H₂ in aqueous solutions via light-induced interprotein electron transfer reactions (TON > 2500 H₂/FNR), providing insight about using native protein-protein interactions as a method for fuel generation.

View Accepted Manuscript (DOE)

Research Organization:: Argonne National Laboratory (ANL), Argonne, IL (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Basic Energy Sciences (BES). Chemical Sciences, Geosciences & Biosciences Division

Grant/Contract Number:: AC02-06CH11357

OSTI ID:: 1606243

Journal Information:: Photosynthesis Research, Journal Name: Photosynthesis Research Journal Issue: 2 Vol. 143; ISSN 0166-8595

Publisher:: SpringerCopyright Statement

Country of Publication:: United States

Language:: English

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