Structure and dynamics of lipid membranes interacting with antivirulence end-phosphorylated polyethylene glycol block copolymers

Yu, Jing; Mao, Jun; Nagao, Michihiro; Bu, Wei; Lin, Binhua; Hong, Kunlun; Jiang, Zhang; Liu, Yun; Qian, Shuo; Tirrell, Matthew; Chen, Wei

doi:10.1039/c9sm01642b

Structure and dynamics of lipid membranes interacting with antivirulence end-phosphorylated polyethylene glycol block copolymers

Journal Article · Wed Jan 01 00:00:00 EST 2020 · Soft Matter

DOI:https://doi.org/10.1039/c9sm01642b· OSTI ID:1605989

^[1]; Mao, Jun ^[1]; ^[2]; ^[3]; ^[3]; ^[4]; Jiang, Zhang ^[5]; Liu, Yun ^[6]; ^[7]; ^[1]; ^[1]

Univ. of Chicago, IL (United States); Argonne National Lab. (ANL), Argonne, IL (United States). Materials Science Division
National Inst. of Standards and Technology (NIST), Gaithersburg, MD (United States); Indiana Univ., Bloomington, IN (United States)
Univ. of Chicago, IL (United States)
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). Center for Nanophase Materials Science (CNMS)
Argonne National Lab. (ANL), Argonne, IL (United States). Advanced Photon Source (APS)
National Inst. of Standards and Technology (NIST), Gaithersburg, MD (United States). Center for Neutron Research
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)

The structure and dynamics of lipid membranes in the presence of extracellular macromolecules are critical for cell membrane functions and many pharmaceutical applications. The pathogen virulence-suppressing end-phosphorylated polyethylene glycol (PEG) triblock copolymer (Pi-ABAPEG) markedly changes the interactions with lipid vesicle membranes and prevents PEG-induced vesicle phase separation in contrast to the unphosphorylated copolymer (ABAPEG). Pi-ABAPEG weakly absorbs on the surface of lipid vesicle membranes and slightly changes the structure of 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC) unilamellar vesicles at 37 °C, as evidenced by small angle neutron scattering. X-ray reflectivity measurements confirm the weak adsorption of Pi-ABAPEG on DMPC monolayer, resulting in a more compact DMPC monolayer structure. Neutron spin-echo results show that the adsorption of Pi-ABAPEG on DMPC vesicle membranes increases the membrane bending modulus $$κ$$.

View Accepted Manuscript (DOE)

Research Organization:: Argonne National Laboratory (ANL), Argonne, IL (United States); Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)

Sponsoring Organization:: National Science Foundation (NSF); U.S. Department of Commerce; USDOE; USDOE Office of Science (SC), Basic Energy Sciences (BES) (SC-22). Materials Sciences & Engineering Division

Grant/Contract Number:: AC02-06CH11357; AC05-00OR22725

OSTI ID:: 1605989

Alternate ID(s):: OSTI ID: 1777825
OSTI ID: 1579770

Journal Information:: Soft Matter, Journal Name: Soft Matter Journal Issue: 4 Vol. 16; ISSN SMOABF; ISSN 1744-683X

Publisher:: Royal Society of ChemistryCopyright Statement

Country of Publication:: United States

Language:: English

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