The importance of hydrogen and formate transfer for syntrophic fatty, aromatic and alicyclic metabolism [plus supplemental information]

Sieber, Jessica R.; Le, Huynh M.; McInerney, Michael J.

doi:10.1111/1462-2920.12269

Title: The importance of hydrogen and formate transfer for syntrophic fatty, aromatic and alicyclic metabolism [plus supplemental information]

Journal Article · Wed Sep 04 00:00:00 EDT 2013 · Environmental Microbiology

DOI:https://doi.org/10.1111/1462-2920.12269· OSTI ID:1604793

Sieber, Jessica R. ^[1]; Le, Huynh M. ^[1]; McInerney, Michael J. ^[1]

Univ. of Oklahoma, Norman, OK (United States)

In this study, we used a combination of genomic, transcriptional, and enzymatic analyses to determine the mechanism of interspecies electron transfer by two model syntrophic microorganisms, Syntrophomonas wolfei and Syntrophus aciditrophicus. Both organisms contain multiple hydrogenase and formate dehydrogenase genes, but lack genes for outer membrane cytochromes and nanowire formation. Syntrophically grown cells and cell-free extracts of S. aciditrophicus and S. wolfei had both hydrogenase and formate dehydrogenase activities. Butyrate metabolism and CH₄ production by washed cell suspensions of S. wolfei and Methanospirillum hungatei were inhibited by hydrogenase inhibitors (cyanide and carbon monoxide), but not by a formate dehydrogenase inhibitor (hypophosphite). Syntrophic benzoate oxidation and CH₄ production by washed cell suspensions of S. aciditrophicus and M. hungatei were inhibited by hypophosphite, but not cyanide and carbon monoxide. All three inhibitors halted syntrophic cyclohexane-1-carboxylate metabolism. Two hydrogenase genes, hydA1 and hydA2, were more highly expressed when S. wolfei was grown syntrophically. S. aciditrophicus expressed multiple hydrogenase and formate dehydrogenase genes during syntrophic benzoate and cyclohexane-1-carboxylate growth, one of which (fdhA2) was highly differentially expressed during syntrophic benzoate growth. Therefore, these syntrophic microorganisms have flexible metabolisms that allow them to use either H₂ or formate transfer depending on the substrate involved.

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Research Organization:: Univ. of Oklahoma, Norman, OK (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Basic Energy Sciences (BES). Chemical Sciences, Geosciences, and Biosciences Division

Grant/Contract Number:: FG02-96ER20214

OSTI ID:: 1604793

Journal Information:: Environmental Microbiology, Vol. 16, Issue 1; ISSN 1462-2912

Publisher:: WileyCopyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 67 works

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Title: The importance of hydrogen and formate transfer for syntrophic fatty, aromatic and alicyclic metabolism [plus supplemental information]

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