An Activity-Based Methionine Bioconjugation Approach To Developing Proximity-Activated Imaging Reporters

Ohata, Jun; Krishnamoorthy, Lakshmi; Gonzalez, Monica A.; Xiao, Tong; Iovan, Diana A.; Toste, F. Dean; Miller, Evan W.; Chang, Christopher J.

doi:10.1021/acscentsci.9b01038

An Activity-Based Methionine Bioconjugation Approach To Developing Proximity-Activated Imaging Reporters

Journal Article · Tue Jan 07 00:00:00 EST 2020 · ACS Central Science

DOI:https://doi.org/10.1021/acscentsci.9b01038· OSTI ID:1603592

^[1]; ^[1]; Gonzalez, Monica A. ^[1]; ^[1]; ^[1]; ^[1]; ^[1]; ^[1]

Univ. of California, Berkeley, CA (United States)

Chemical probes that report on protein activity, rather than protein abundance, with spatial and temporal resolution can enable studies of their native function in biological contexts as well as provide opportunities for developing new types of biochemical reporters. Here we present a sensing platform, termed proximity-activated imaging reporter (PAIR), which combines activity-based methionine bioconjugation and antibody labeling with proximity-dependent oligonucleotide-based amplification to monitor dynamic changes of a given analyte in cells and animals through context-dependent methionine labeling of specific protein targets. We establish this PAIR method to develop sensors for imaging reactive oxygen species (ROS) and calcium ions through oxaziridine-directed labeling of reactive methionine residues on β-actin and calmodulin (CaM), respectively, where the extent of methionine bioconjugation on these protein targets can serve as an indicator of oxidative stress or calcium status. In particular, application of PAIR to activity-based CaM detection provides a method for imaging integrated calcium activity in both in vitro cell and in vivo zebrafish models. By relying on native protein biochemistry, PAIR enables redox and metal imaging without introduction of external small molecules or genetically encoded indicators that can potentially buffer the natural/existing pools. This approach can be potentially generalized to target a broader range of analytes by pairing appropriate activity-based protein probes with protein detection reagents in a proximity-driven manner, providing a starting point not only for designing new sensors but also for monitoring endogenous activity of specific protein targets in biological specimens with spatial and temporal fidelity.

View Accepted Manuscript (DOE)

Research Organization:: Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)

Sponsoring Organization:: USDOE Office of Science (SC)

Grant/Contract Number:: AC02-05CH11231

OSTI ID:: 1603592

Journal Information:: ACS Central Science, Journal Name: ACS Central Science Journal Issue: 1 Vol. 6; ISSN 2374-7943

Publisher:: American Chemical Society (ACS)Copyright Statement

Country of Publication:: United States

Language:: English

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