Structure of the gene for the human myeloid IgA Fc receptor (CD89)

Journal Article · Tue Aug 01 04:00:00 EDT 1995 · Journal of Immunology

OSTI ID:160148

Wit, T P.M. de; Morton, H C; Capel, P J.A. ^[1]

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1. Univ. Hospital of Utrecht (Netherlands); and others

A better understanding of IgA`s role in immunity requires insight in IgAR complexity. We have now isolated, characterized, and sequenced the gene encoding the prototypic human myeloid IgA FcR (CD89). The gene consists of five exons and spans approximately 12 kilobase pairs. The leader peptide is encoded by two exons, the second of which is 36 bp long and specifies the predicted peptidase cleavage site. A similar, but shorter (21 bp) mini-exon has been found in the FcR for IgC (Fc{gamma}R) genes, and the FcR for IgE (Fc{epsilon}RI{alpha}) gene (human and rodent). The third and fourth exons code for two homologous Ig-like domains. The final exon encodes a short extracellular region, a hydrophobic transmembrane region, and a short cytoplasmic tail. The sequence of the 5{prime}-flanking region was determined, and one major and several minor transcription initiation sites were mapped by primer extension studies. A putative TATA box was located at an appropriate location relative to the start site. Southern blot analyses of genomic DNA confirm the restriction map generated from cloned DNA. These data define the Fc{alpha}R gene as a distantly related member of the IgR gene family.

• OSTI ID: 160148
• Journal Information: Journal of Immunology, Journal Name: Journal of Immunology Journal Issue: 3 Vol. 155; ISSN JOIMA3; ISSN 0022-1767
• Country of Publication: United States
• Language: English

Related Subjects

55 BIOLOGY AND MEDICINE
BASIC STUDIES
ANTIBODIES
DNA SEQUENCING
DNA-CLONING
EXONS
GENE REGULATION
GENES
GENETIC MAPPING
IMMUNOGLOBULINS
PEPTIDES
RECEPTORS
STRUCTURE-ACTIVITY RELATIONSHIPS
TRANSCRIPTION