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Title: Modified host cells having tolerance to α-olefins

Patent ·
OSTI ID:1600420
; ;

The present invention provides methods for improving microbial tolerance to alpha olefin compounds, host cells having increased tolerance to such compounds, and method of using the host cells to produce alpha olefin compounds.

Research Organization:
Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)
Sponsoring Organization:
USDOE
DOE Contract Number:
AC02-05CH11231
Assignee:
The Regents of the University of California (Oakland, CA); Total Marketing Services (Puteaux, FR)
Patent Number(s):
10,513,713
Application Number:
15/307,783
OSTI ID:
1600420
Resource Relation:
Patent File Date: 04/29/2015
Country of Publication:
United States
Language:
English

References (24)

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Site-Directed Mutagenesis Reveals Putative Substrate Binding Residues in the Escherichia coli RND Efflux Pump AcrB journal October 2008
Sequential Mechanism of Assembly of Multidrug Efflux Pump AcrAB-TolC journal April 2011
Conversion of a β-Ketoacyl Synthase to a Malonyl Decarboxylase by Replacement of the Active-Site Cysteine with Glutamine journal September 1999
Engineering microbial biofuel tolerance and export using efflux pumps journal January 2011
Directed evolution of an E. coli inner membrane transporter for improved efflux of biofuel molecules journal January 2013
Extramembrane Central Pore of Multidrug Exporter AcrB in Escherichia coli Plays an Important Role in Drug Transport journal January 2004
Products and Methods for In Vivo Secretion of Monatin patent-application May 2007
Methods for Screening for acrAB Efflux Pump Inhibitors patent-application November 2005
Random Mutagenesis of the Multidrug Transporter AcrB from Escherichia coli for Identification of Putative Target Residues of Efflux Pump Inhibitors journal November 2014
Conformation of the AcrB Multidrug Efflux Pump in Mutants of the Putative Proton Relay Pathway journal October 2006
Mechanism of recognition of compounds of diverse structures by the multidrug efflux pump AcrB of Escherichia coli journal March 2010
Consequences of Membrane Protein Overexpression in Escherichia coli journal April 2007
Identification of Dehalobacter reductive dehalogenases that catalyse dechlorination of chloroform, 1,1,1-trichloroethane and 1,1-dichloroethane journal April 2013
AcrB Trimer Stability and Efflux Activity, Insight from Mutagenesis Studies journal December 2011
Site-Directed Disulfide Cross-Linking Shows that Cleft Flexibility in the Periplasmic Domain Is Needed for the Multidrug Efflux Pump AcrB of Escherichia coli journal December 2007
Methods and Compositions for the Extracellular Transport of Biosynthetic Hydrocarbons and Other Molecules patent-application April 2014
Production and Secretion of Fatty Acids and Fatty Acid Derivatives patent-application March 2013
Vestibules Are Part of the Substrate Path in the Multidrug Efflux Transporter AcrB of Escherichia coli journal August 2011
Modified Host Cells with Efflux Pumps patent-application December 2011
Multidrug binding properties of the AcrB efflux pump characterized by molecular dynamics simulations journal November 2012
The sequence–structure relationship and protein function prediction journal June 2009
Crystal structure of bacterial multidrug efflux transporter AcrB journal October 2002
Melamine Deaminase and Atrazine Chlorohydrolase: 98 Percent Identical but Functionally Different journal April 2001

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59 BASIC BIOLOGICAL SCIENCES
09 BIOMASS FUELS