skip to main content
OSTI.GOV title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Cytosolic Acetyl-CoA Generated by ATP-Citrate Lyase Is Essential for Acetylation of Cell Wall Polysaccharides

Journal Article · · Plant and Cell Physiology
DOI:https://doi.org/10.1093/pcp/pcz178· OSTI ID:1599930
 [1];  [2];  [3];  [2];  [4];  [4]; ORCiD logo [1]
  1. Department of Plant Biology, University of Georgia, Athens, GA 30602, USA
  2. Department of Chemistry, University of Georgia, Athens, GA 30602, USA
  3. Georgia Electron Microscopy, University of Georgia, Athens, GA 30602, USA
  4. Complex Carbohydrate Research Center, University of Georgia, Athens, GA 30602, USA
+ Show Author Affiliations

Abstract Plant cell wall polysaccharides, including xylan, glucomannan, xyloglucan and pectin, are often acetylated. Although a number of acetyltransferases responsible for the acetylation of some of these polysaccharides have been biochemically characterized, little is known about the source of acetyl donors and how acetyl donors are translocated into the Golgi, where these polysaccharides are synthesized. In this report, we investigated roles of ATP-citrate lyase (ACL) that generates cytosolic acetyl-CoA in cell wall polysaccharide acetylation and effects of simultaneous mutations of four Reduced Wall Acetylation (RWA) genes on acetyl-CoA transport into the Golgi in Arabidopsis thaliana. Expression analyses of genes involved in the generation of acetyl-CoA in different subcellular compartments showed that the expression of several ACL genes responsible for cytosolic acetyl-CoA synthesis was elevated in interfascicular fiber cells and induced by secondary wall-associated transcriptional activators. Simultaneous downregulation of the expression of ACL genes was demonstrated to result in a substantial decrease in the degree of xylan acetylation and a severe alteration in secondary wall structure in xylem vessels. In addition, the degree of acetylation of other cell wall polysaccharides, including glucomannan, xyloglucan and pectin, was also reduced. Moreover, Golgi-enriched membrane vesicles isolated from the rwa1/2/3/4 quadruple mutant were found to exhibit a drastic reduction in acetyl-CoA transport activity compared with the wild type. These findings indicate that cytosolic acetyl-CoA generated by ACL is essential for cell wall polysaccharide acetylation and RWAs are required for its transport from the cytosol into the Golgi.

Research Organization:
Univ. of Georgia, Athens, GA (United States). Complex Carbohydrate Research Center
Sponsoring Organization:
USDOE Office of Science (SC), Basic Energy Sciences (BES)
Grant/Contract Number:
SC0015662; FG02-03ER15415
OSTI ID:
1599930
Alternate ID(s):
OSTI ID: 1964042
Journal Information:
Plant and Cell Physiology, Journal Name: Plant and Cell Physiology Vol. 61 Journal Issue: 1; ISSN 0032-0781
Publisher:
Oxford University PressCopyright Statement
Country of Publication:
Japan
Language:
English
Citation Metrics:
Cited by: 10 works
Citation information provided by
Web of Science

References (51)

Demethylesterification of the Primary Wall by PECTIN METHYLESTERASE35 Provides Mechanical Support to the Arabidopsis Stem journal June 2012
Roles of Arabidopsis TBL34 and TBL35 in xylan acetylation and plant growth journal February 2016
Secondary cell wall biosynthesis journal November 2018
The MYB46 Transcription Factor Is a Direct Target of SND1 and Regulates Secondary Wall Biosynthesis in Arabidopsis journal September 2007
Isolation of a Golgi-rich fraction from rat liver journal August 1970
Isolation and structural characterisation of pectin from endocarp of Citrus depressa journal March 2008
Regiospecific Acetylation of Xylan is Mediated by a Group of DUF231-Containing O-Acetyltransferases journal October 2017
Characterization of O-acetyl-(4-O-methylglucurono)xylan isolated from birch and beech journal February 2002
New Insights Into Wall Polysaccharide O-Acetylation journal August 2018
Location of the O-acetyl substituents on a nonasaccharide repeating unit of sycamore extracellular xyloglucan journal February 1988
Substrate Specificity, Mode of Action, and of Inhibition by Organic Acids of Purified Acetylesterase from Sclerotinia Fungus journal May 1967
Substrate Specificity and Kinetic Characterization of Peptidoglycan O -Acetyltransferase B from Neisseria gonorrhoeae journal May 2014
Secondary Cell Walls: Biosynthesis, Patterned Deposition and Transcriptional Regulation journal October 2014
Molecular Characterization of a Heteromeric ATP-Citrate Lyase That Generates Cytosolic Acetyl-Coenzyme A in Arabidopsis journal October 2002
The Four Arabidopsis REDUCED WALL ACETYLATION Genes are Expressed in Secondary Wall-Containing Cells and Required for the Acetylation of Xylan journal June 2011
SND1, a NAC Domain Transcription Factor, Is a Key Regulator of Secondary Wall Synthesis in Fibers of Arabidopsis journal November 2006
Xyloglucan O-acetyltransferases from Arabidopsis thaliana and Populus trichocarpa catalyze acetylation of fucosylated galactose residues on xyloglucan side chains journal August 2018
Cytosolic acetyl-CoA promotes histone acetylation predominantly at H3K27 in Arabidopsis journal September 2017
Cas1p is a membrane protein necessary for the O-acetylation of the Cryptococcus neoformans capsular polysaccharide journal October 2001
A group of Populus trichocarpa DUF231 proteins exhibit differential O-acetyltransferase activities toward xylan journal April 2018
9-O-Acetylation of sialic acids is catalysed by CASD1 via a covalent acetyl-enzyme intermediate journal July 2015
The biomass distribution on Earth journal May 2018
Natural acetylation impacts carbohydrate recovery during deconstruction of Populus trichocarpa wood journal February 2017
Identification and Characterization of O -Acetylpeptidoglycan Esterase:  A Novel Enzyme Discovered in Neisseria gonorrhoeae † journal January 2006
Biosynthesis of the Plant Cell Wall Matrix Polysaccharide Xyloglucan journal April 2016
P. aeruginosa SGNH Hydrolase-Like Proteins AlgJ and AlgX Have Similar Topology but Separate and Distinct Roles in Alginate Acetylation journal August 2014
O-Acetylated Oligosaccharides from Pectins of Potato Tuber Cell Walls journal April 1997
VASCULAR-RELATED NAC-DOMAIN6 and VASCULAR-RELATED NAC-DOMAIN7 Effectively Induce Transdifferentiation into Xylem Vessel Elements under Control of an Induction System journal May 2010
Arabidopsis Fragile Fiber8 , Which Encodes a Putative Glucuronyltransferase, Is Essential for Normal Secondary Wall Synthesis journal November 2005
The protein acetylome and the regulation of metabolism journal July 2012
A xylosyltransferase that synthesizes β-(1→4)-xylans in wheat (Triticum aestivum L.) seedlings journal June 2001
Colony Organization in the Green Alga Botryococcus braunii (Race B) Is Specified by a Complex Extracellular Matrix journal August 2012
Disruption of Cortical Microtubules by Overexpression of Green Fluorescent Protein-Tagged alpha-Tubulin 6 Causes a Marked Reduction in Cell Wall Synthesis journal January 2006
Three Arabidopsis DUF579 Domain-Containing GXM Proteins are Methyltransferases Catalyzing 4-O-Methylation of Glucuronic Acid on Xylan journal October 2012
Isolation and characterization of O-acetylated glucomannans from aspen and birch wood journal March 2003
Biochemical characterization of rice xylan O-acetyltransferases journal March 2018
Reduced Wall Acetylation Proteins Play Vital and Distinct Roles in Cell Wall O -Acetylation in Arabidopsis journal September 2013
The Role of the Plant-Specific ALTERED XYLOGLUCAN9 Protein in Arabidopsis Cell Wall Polysaccharide O- Acetylation journal February 2015
A Xyloglucan Backbone 6-O-Acetyltransferase from Brachypodium distachyon Modulates Xyloglucan Xylosylation journal April 2016
In vitro characterization of the antivirulence target of Gram-positive pathogens, peptidoglycan O-acetyltransferase A (OatA) journal October 2017
Mutations of Arabidopsis TBL32 and TBL33 Affect Xylan Acetylation and Secondary Wall Deposition journal January 2016
Xylan O-Acetylation Impacts Xylem Development and Enzymatic Recalcitrance as Indicated by the Arabidopsis Mutant tbl29 journal July 2013
O -Acetylation of Arabidopsis Hemicellulose Xyloglucan Requires AXY4 or AXY4L, Proteins with a TBL and DUF231 Domain journal November 2011
The Arabidopsis DUF231 Domain-Containing Protein ESK1 Mediates 2-O- and 3-O-Acetylation of Xylosyl Residues in Xylan journal May 2013
Evolutionary origin of O ‐acetyltransferases responsible for glucomannan acetylation in land plants journal June 2019
TBL3 and TBL31, Two Arabidopsis DUF231 Domain Proteins, are Required for 3- O -Monoacetylation of Xylan journal November 2015
Two Arabidopsis proteins synthesize acetylated xylan in vitro journal September 2014
Characterization of pectin methyltransferase from soybean hypocotyls journal April 2000
TBL10 is required for O -acetylation of pectic rhamnogalacturonan-I in Arabidopsis thaliana journal September 2018
Reverse Genetic Characterization of Cytosolic Acetyl-CoA Generation by ATP-Citrate Lyase in Arabidopsis journal December 2004
Structural and Functional Characterization of Pseudomonas aeruginosa AlgX : ROLE OF AlgX IN ALGINATE ACETYLATION journal June 2013

Similar Records

Cytosolic Acetyl-CoA Generated by ATP-Citrate Lyase Is Essential for Acetylation of Cell Wall Polysaccharides
Journal Article · Tue Sep 10 00:00:00 EDT 2019 · Plant and Cell Physiology · OSTI ID:1599930
+4 more
Acetylation of homogalacturonan and rhamnogalacturonan‐I is catalyzed by a suite of trichome birefringence‐like proteins
Journal Article · Tue Nov 07 00:00:00 EST 2023 · The Plant Journal · OSTI ID:1599930
+1 more
ATP citrate lyase mediated cytosolic acetyl-CoA biosynthesis increases mevalonate production in Saccharomyces cerevisiae
Journal Article · Thu Mar 03 00:00:00 EST 2016 · Microbial Cell Factories · OSTI ID:1599930
+3 more

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
Acetyl-CoA
Acetylation
Acetyltransferase
Arabidopsis
Cell wall