Architecture of the chromatin remodeler RSC and insights into its nucleosome engagement

Patel, Avinash B.; Moore, Camille M.; Greber, Basil J.; Luo, Jie; Zukin, Stefan A.; Ranish, Jeff; Nogales, Eva

doi:10.7554/eLife.54449

Architecture of the chromatin remodeler RSC and insights into its nucleosome engagement

Journal Article · Mon Dec 30 00:00:00 EST 2019 · eLife

DOI:https://doi.org/10.7554/eLife.54449· OSTI ID:1599828

^[1]; Moore, Camille M. ^[2]; ^[1]; Luo, Jie ^[3]; Zukin, Stefan A. ^[2]; Ranish, Jeff ^[3]; ^[1]

Univ. of California, Berkeley, CA (United States); Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
Univ. of California, Berkeley, CA (United States)
The Inst. for Systems Biology, Seattle, WA (United States)

Eukaryotic DNA is packaged into nucleosome arrays, which are repositioned by chromatin remodeling complexes to control DNA accessibility. The Saccharomyces cerevisiae RSC (Remodeling the Structure of Chromatin) complex, a member of the SWI/SNF chromatin remodeler family, plays critical roles in genome maintenance, transcription, and DNA repair. Here, we report cryo-electron microscopy (cryo-EM) and crosslinking mass spectrometry (CLMS) studies of yeast RSC complex and show that RSC is composed of a rigid tripartite core and two flexible lobes. The core structure is scaffolded by an asymmetric Rsc8 dimer and built with the evolutionarily conserved subunits Sfh1, Rsc6, Rsc9 and Sth1. The flexible ATPase lobe, composed of helicase subunit Sth1, Arp7, Arp9 and Rtt102, is anchored to this core by the N-terminus of Sth1. Our cryo-EM analysis of RSC bound to a nucleosome core particle shows that in addition to the expected nucleosome-Sth1 interactions, RSC engages histones and nucleosomal DNA through one arm of the core structure, composed of the Rsc8 SWIRM domains, Sfh1 and Npl6. Our findings provide structural insights into the conserved assembly process for all members of the SWI/SNF family of remodelers, and illustrate how RSC selects, engages, and remodels nucleosomes.

View Accepted Manuscript (DOE)

Research Organization:: Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)

Sponsoring Organization:: National Institute of General Medical Sciences (NIGMS); National Institutes of Health (NIH); USDOE Office of Science (SC), Biological and Environmental Research (BER) (SC-23)

Grant/Contract Number:: AC02-05CH11231

OSTI ID:: 1599828

Journal Information:: eLife, Journal Name: eLife Vol. 8; ISSN 2050-084X

Publisher:: eLife Sciences Publications, Ltd.Copyright Statement

Country of Publication:: United States

Language:: English

References (83)

UCSF Chimera?A visualization system for exploratory research and analysis Pettersen, Eric F.; Goddard, Thomas D.; Huang, Conrad C. Journal of Computational Chemistry, Vol. 25, Issue 13 https://doi.org/10.1002/jcc.20084	journal	January 2004
UCSF ChimeraX: Meeting modern challenges in visualization and analysis: UCSF ChimeraX Visualization System Goddard, Thomas D.; Huang, Conrad C.; Meng, Elaine C. Protein Science, Vol. 27, Issue 1 https://doi.org/10.1002/pro.3235	journal	September 2017
Protein secondary structure prediction based on position-specific scoring matrices 1 1Edited by G. Von Heijne Jones, David T. Journal of Molecular Biology, Vol. 292, Issue 2 https://doi.org/10.1006/jmbi.1999.3091	journal	September 1999
The Tandem Affinity Purification (TAP) Method: A General Procedure of Protein Complex Purification Puig, Oscar; Caspary, Friederike; Rigaut, Guillaume Methods, Vol. 24, Issue 3 https://doi.org/10.1006/meth.2001.1183	journal	July 2001
Architecture of SWI/SNF chromatin remodeling complex Zhang, Zhihui; Wang, Xuejuan; Xin, Jiyu Protein & Cell, Vol. 9, Issue 12 https://doi.org/10.1007/s13238-018-0524-9	journal	March 2018
RSC, an Essential, Abundant Chromatin-Remodeling Complex Cairns, Bradley R.; Lorch, Yahli; Li, Yang Cell, Vol. 87, Issue 7 https://doi.org/10.1016/S0092-8674(00)81820-6	journal	December 1996
Two Functionally Distinct Forms of the RSC Nucleosome-Remodeling Complex, Containing Essential AT Hook, BAH, and Bromodomains Cairns, Bradley R.; Schlichter, Alisha; Erdjument-Bromage, Hediye Molecular Cell, Vol. 4, Issue 5 https://doi.org/10.1016/S1097-2765(00)80382-2	journal	November 1999
A Rsc3/Rsc30 Zinc Cluster Dimer Reveals Novel Roles for the Chromatin Remodeler RSC in Gene Expression and Cell Cycle Control Angus-Hill, Melinda L.; Schlichter, Alisha; Roberts, Douglas Molecular Cell, Vol. 7, Issue 4 https://doi.org/10.1016/S1097-2765(01)00219-2	journal	April 2001
X-Ray Structures of the Sulfolobus solfataricus SWI2/SNF2 ATPase Core and Its Complex with DNA Dürr, Harald; Körner, Christian; Müller, Marisa Cell, Vol. 121, Issue 3 https://doi.org/10.1016/j.cell.2005.03.026	journal	May 2005
Histone Recognition and Large-Scale Structural Analysis of the Human Bromodomain Family Filippakopoulos, Panagis; Picaud, Sarah; Mangos, Maria Cell, Vol. 149, Issue 1 https://doi.org/10.1016/j.cell.2012.02.013	journal	March 2012
Genome-wide Nucleosome Specificity and Directionality of Chromatin Remodelers Yen, Kuangyu; Vinayachandran, Vinesh; Batta, Kiran Cell, Vol. 149, Issue 7 https://doi.org/10.1016/j.cell.2012.04.036	journal	June 2012
Genomic Nucleosome Organization Reconstituted with Pure Proteins Krietenstein, Nils; Wal, Megha; Watanabe, Shinya Cell, Vol. 167, Issue 3 https://doi.org/10.1016/j.cell.2016.09.045	journal	October 2016
Modular Organization and Assembly of SWI/SNF Family Chromatin Remodeling Complexes Mashtalir, Nazar; D’Avino, Andrew R.; Michel, Brittany C. Cell, Vol. 175, Issue 5 https://doi.org/10.1016/j.cell.2018.09.032	journal	November 2018
Recurrent SMARCB1 Mutations Reveal a Nucleosome Acidic Patch Interaction Site That Potentiates mSWI/SNF Complex Chromatin Remodeling Valencia, Alfredo M.; Collings, Clayton K.; Dao, Hai T. Cell, Vol. 179, Issue 6 https://doi.org/10.1016/j.cell.2019.10.044	journal	November 2019
Composition and Function of Mutant Swi/Snf Complexes Dutta, Arnob; Sardiu, Mihaela; Gogol, Madelaine Cell Reports, Vol. 18, Issue 9 https://doi.org/10.1016/j.celrep.2017.01.058	journal	February 2017
Loss of Snf5 Induces Formation of an Aberrant SWI/SNF Complex Sen, Payel; Luo, Jie; Hada, Arjan Cell Reports, Vol. 18, Issue 9 https://doi.org/10.1016/j.celrep.2017.02.017	journal	February 2017
Dynamics of Chromatin and Transcription during Transient Depletion of the RSC Chromatin Remodeling Complex Klein-Brill, Avital; Joseph-Strauss, Daphna; Appleboim, Alon Cell Reports, Vol. 26, Issue 1 https://doi.org/10.1016/j.celrep.2018.12.020	journal	January 2019
Structural Insights into BAF47 and BAF155 Complex Formation Yan, Li; Xie, Si; Du, Yongming Journal of Molecular Biology, Vol. 429, Issue 11 https://doi.org/10.1016/j.jmb.2017.04.008	journal	June 2017
Automated molecular microscopy: The new Leginon system Suloway, Christian; Pulokas, James; Fellmann, Denis Journal of Structural Biology, Vol. 151, Issue 1 https://doi.org/10.1016/j.jsb.2005.03.010	journal	July 2005
iMODFIT: Efficient and robust flexible fitting based on vibrational analysis in internal coordinates Lopéz-Blanco, José Ramón; Chacón, Pablo Journal of Structural Biology, Vol. 184, Issue 2 https://doi.org/10.1016/j.jsb.2013.08.010	journal	November 2013
Gctf: Real-time CTF determination and correction Zhang, Kai Journal of Structural Biology, Vol. 193, Issue 1 https://doi.org/10.1016/j.jsb.2015.11.003	journal	January 2016
A Library of Yeast Transcription Factor Motifs Reveals a Widespread Function for Rsc3 in Targeting Nucleosome Exclusion at Promoters Badis, Gwenael; Chan, Esther T.; van Bakel, Harm Molecular Cell, Vol. 32, Issue 6 https://doi.org/10.1016/j.molcel.2008.11.020	journal	December 2008
Regulation of DNA Translocation Efficiency within the Chromatin Remodeler RSC/Sth1 Potentiates Nucleosome Sliding and Ejection Clapier, Cedric R.; Kasten, Margaret M.; Parnell, Timothy J. Molecular Cell, Vol. 62, Issue 3 https://doi.org/10.1016/j.molcel.2016.03.032	journal	May 2016
Structural Studies of the Human PBAF Chromatin-Remodeling Complex Leschziner, Andres E.; Lemon, Bryan; Tjian, Robert Structure, Vol. 13, Issue 2 https://doi.org/10.1016/j.str.2004.12.008	journal	February 2005
The SWI/SNF Subunit INI1 Contains an N-Terminal Winged Helix DNA Binding Domain that Is a Target for Mutations in Schwannomatosis Allen, Mark D.; Freund, Stefan M. V.; Zinzalla, Giovanna Structure, Vol. 23, Issue 7 https://doi.org/10.1016/j.str.2015.04.021	journal	July 2015
Fast, scalable generation of high‐quality protein multiple sequence alignments using Clustal Omega Sievers, Fabian; Wilm, Andreas; Dineen, David Molecular Systems Biology, Vol. 7, Issue 1 https://doi.org/10.1038/msb.2011.75	journal	January 2011
Mechanism of chromatin remodelling revealed by the Snf2-nucleosome structure Liu, Xiaoyu; Li, Meijing; Xia, Xian Nature, Vol. 544, Issue 7651 https://doi.org/10.1038/nature22036	journal	April 2017
MotionCor2: anisotropic correction of beam-induced motion for improved cryo-electron microscopy Zheng, Shawn Q.; Palovcak, Eugene; Armache, Jean-Paul Nature Methods, Vol. 14, Issue 4 https://doi.org/10.1038/nmeth.4193	journal	February 2017
Addressing preferred specimen orientation in single-particle cryo-EM through tilting Tan, Yong Zi; Baldwin, Philip R.; Davis, Joseph H. Nature Methods, Vol. 14, Issue 8 https://doi.org/10.1038/nmeth.4347	journal	July 2017
Structural analysis of the yeast SWI/SNF chromatin remodeling complex Smith, Corey L.; Horowitz-Scherer, Rachel; Flanagan, Joan F. Nature Structural Biology, Vol. 10, Issue 2 https://doi.org/10.1038/nsb888	journal	January 2003
Structure of a RSC–nucleosome complex and insights into chromatin remodeling Chaban, Yuriy; Ezeokonkwo, Chukwudi; Chung, Wen-Hsiang Nature Structural & Molecular Biology, Vol. 15, Issue 12 https://doi.org/10.1038/nsmb.1524	journal	November 2008
Structure of chromatin remodeler Swi2/Snf2 in the resting state Xia, Xian; Liu, Xiaoyu; Li, Tong Nature Structural & Molecular Biology, Vol. 23, Issue 8 https://doi.org/10.1038/nsmb.3259	journal	July 2016
Swi3p controls SWI/SNF assembly and ATP-dependent H2A-H2B displacement Yang, Xiaofang; Zaurin, Roser; Beato, Miguel Nature Structural & Molecular Biology, Vol. 14, Issue 6 https://doi.org/10.1038/nsmb1238	journal	May 2007
PARP1 exhibits enhanced association and catalytic efficiency with γH2A.X-nucleosome Sharma, Deepti; De Falco, Louis; Padavattan, Sivaraman Nature Communications, Vol. 10, Issue 1 https://doi.org/10.1038/s41467-019-13641-0	journal	December 2019
Synthetic antibodies against BRIL as universal fiducial marks for single−particle cryoEM structure determination of membrane proteins Mukherjee, Somnath; Erramilli, Satchal K.; Ammirati, Mark Nature Communications, Vol. 11, Issue 1 https://doi.org/10.1038/s41467-020-15363-0	journal	March 2020
Errors in the deposited SFTSV L protein structure Cusack, Stephen; Rosenthal, Maria Nature Microbiology, Vol. 6, Issue 5 https://doi.org/10.1038/s41564-021-00901-3	journal	April 2021
Mechanism of DNA translocation underlying chromatin remodelling by Snf2 Li, Meijing; Xia, Xian; Tian, Yuanyuan Nature, Vol. 567, Issue 7748 https://doi.org/10.1038/s41586-019-1029-2	journal	March 2019
Structure of SWI/SNF chromatin remodeller RSC bound to a nucleosome Wagner, Felix R.; Dienemann, Christian; Wang, Haibo Nature, Vol. 579, Issue 7799 https://doi.org/10.1038/s41586-020-2088-0	journal	March 2020
Visualization of the mechanosensitive ion channel MscS under membrane tension Zhang, Yixiao; Daday, Csaba; Gu, Ruo-Xu Nature, Vol. 590, Issue 7846 https://doi.org/10.1038/s41586-021-03196-w	journal	February 2021
Software tools for automated transmission electron microscopy Schorb, Martin; Haberbosch, Isabella; Hagen, Wim J. H. Nature Methods, Vol. 16, Issue 6 https://doi.org/10.1038/s41592-019-0396-9	journal	May 2019
Tandem bromodomains in the chromatin remodeler RSC recognize acetylated histone H3 Lys14 Kasten, Margaret; Szerlong, Heather; Erdjument-Bromage, Hediye The EMBO Journal, Vol. 23, Issue 6 https://doi.org/10.1038/sj.emboj.7600143	journal	March 2004
Structure and function of the SWIRM domain, a conserved protein module found in chromatin regulatory complexes Da, Guoping; Lenkart, Jeffrey; Zhao, Kehao Proceedings of the National Academy of Sciences, Vol. 103, Issue 7 https://doi.org/10.1073/pnas.0510949103	journal	February 2006
Conformational flexibility in the chromatin remodeler RSC observed by electron microscopy and the orthogonal tilt reconstruction method Leschziner, A. E.; Saha, A.; Wittmeyer, J. Proceedings of the National Academy of Sciences, Vol. 104, Issue 12 https://doi.org/10.1073/pnas.0700706104	journal	March 2007
Histone density is maintained during transcription mediated by the chromatin remodeler RSC and histone chaperone NAP1 in vitro Kuryan, B. G.; Kim, J.; Tran, N. N. H. Proceedings of the National Academy of Sciences, Vol. 109, Issue 6 https://doi.org/10.1073/pnas.1109994109	journal	January 2012
Structure of an actin-related subcomplex of the SWI/SNF chromatin remodeler Schubert, Heidi L.; Wittmeyer, Jacqueline; Kasten, Margaret M. Proceedings of the National Academy of Sciences, Vol. 110, Issue 9 https://doi.org/10.1073/pnas.1215379110	journal	February 2013
Chromatin potentiates transcription Nagai, Shigeki; Davis, Ralph E.; Mattei, Pierre Jean Proceedings of the National Academy of Sciences, Vol. 114, Issue 7 https://doi.org/10.1073/pnas.1620312114	journal	January 2017
Structural analysis of the RSC chromatin-remodeling complex Asturias, F. J.; Chung, W. -H.; Kornberg, R. D. Proceedings of the National Academy of Sciences, Vol. 99, Issue 21 https://doi.org/10.1073/pnas.162504299	journal	October 2002
Acetylated Histone Tail Peptides Induce Structural Rearrangements in the RSC Chromatin Remodeling Complex Skiniotis, Georgios; Moazed, Danesh; Walz, Thomas Journal of Biological Chemistry, Vol. 282, Issue 29 https://doi.org/10.1074/jbc.C700081200	journal	May 2007
Histone H3 Lysine 14 (H3K14) Acetylation Facilitates DNA Repair in a Positioned Nucleosome by Stabilizing the Binding of the Chromatin Remodeler RSC (Remodels Structure of Chromatin) Duan, Ming-Rui; Smerdon, Michael J. Journal of Biological Chemistry, Vol. 289, Issue 12 https://doi.org/10.1074/jbc.M113.540732	journal	February 2014
DISOPRED3: precise disordered region predictions with annotated protein-binding activity Jones, David T.; Cozzetto, Domenico Bioinformatics, Vol. 31, Issue 6 https://doi.org/10.1093/bioinformatics/btu744	journal	November 2014
The HHpred interactive server for protein homology detection and structure prediction Soding, J.; Biegert, A.; Lupas, A. N. Nucleic Acids Research, Vol. 33, Issue Web Server https://doi.org/10.1093/nar/gki408	journal	July 2005
Alteration of the nucleosomal DNA path in the crystal structure of a human nucleosome core particle Tsunaka, Y. Nucleic Acids Research, Vol. 33, Issue 10 https://doi.org/10.1093/nar/gki663	journal	June 2005
Functional interplay between chromatin remodeling complexes RSC, SWI/SNF and ISWI in regulation of yeast heat shock genes Erkina, T. Y.; Zou, Y.; Freeling, S. Nucleic Acids Research, Vol. 38, Issue 5 https://doi.org/10.1093/nar/gkp1130	journal	December 2009
A genome-wide screen for essential yeast genes that affect telomere length maintenance Ungar, L.; Yosef, N.; Sela, Y. Nucleic Acids Research, Vol. 37, Issue 12 https://doi.org/10.1093/nar/gkp259	journal	April 2009
ConSurf 2010: calculating evolutionary conservation in sequence and structure of proteins and nucleic acids Ashkenazy, H.; Erez, E.; Martz, E. Nucleic Acids Research, Vol. 38, Issue Web Server https://doi.org/10.1093/nar/gkq399	journal	May 2010
Histone H3 tail acetylation modulates ATP-dependent remodeling through multiple mechanisms Chatterjee, Nilanjana; Sinha, Divya; Lemma-Dechassa, Mekonnen Nucleic Acids Research, Vol. 39, Issue 19 https://doi.org/10.1093/nar/gkr535	journal	July 2011
Scalable web services for the PSIPRED Protein Analysis Workbench Buchan, Daniel W. A.; Minneci, Federico; Nugent, Tim C. O. Nucleic Acids Research, Vol. 41, Issue W1 https://doi.org/10.1093/nar/gkt381	journal	June 2013
The BAH domain of Rsc2 is a histone H3 binding domain Chambers, Anna L.; Pearl, Laurence H.; Oliver, Antony W. Nucleic Acids Research, Vol. 41, Issue 19 https://doi.org/10.1093/nar/gkt662	journal	July 2013
ConSurf 2016: an improved methodology to estimate and visualize evolutionary conservation in macromolecules Ashkenazy, Haim; Abadi, Shiran; Martz, Eric Nucleic Acids Research, Vol. 44, Issue W1 https://doi.org/10.1093/nar/gkw408	journal	May 2016
The Pfam protein families database in 2019 El-Gebali, Sara; Mistry, Jaina; Bateman, Alex Nucleic Acids Research, Vol. 47, Issue D1 https://doi.org/10.1093/nar/gky995	journal	October 2018
The PSIPRED Protein Analysis Workbench: 20 years on Buchan, Daniel W. A.; Jones, David T. Nucleic Acids Research, Vol. 47, Issue W1 https://doi.org/10.1093/nar/gkz297	journal	April 2019
Structure and function of the SWIRM domain, a conserved protein module found in chromatin regulatory complexes Marmorstein, Ronen; Da, Guoping; Lenkart, Jeffrey The FASEB Journal, Vol. 20, Issue 4 https://doi.org/10.1096/fasebj.20.4.a34-e	journal	March 2006
Diversity and specialization of mammalian SWI/SNF complexes. Wang, W.; Xue, Y.; Zhou, S. Genes & Development, Vol. 10, Issue 17 https://doi.org/10.1101/gad.10.17.2117	journal	September 1996
Genome-wide location and regulated recruitment of the RSC nucleosome-remodeling complex Ng, H. H. Genes & Development, Vol. 16, Issue 7 https://doi.org/10.1101/gad.978902	journal	April 2002
Chromatin remodeling by RSC involves ATP-dependent DNA translocation Saha, A. Genes & Development, Vol. 16, Issue 16 https://doi.org/10.1101/gad.995002	journal	August 2002
Features and development of Coot Emsley, P.; Lohkamp, B.; Scott, W. G. Acta Crystallographica Section D Biological Crystallography, Vol. 66, Issue 4 https://doi.org/10.1107/S0907444910007493	journal	March 2010
A Bayesian approach to beam-induced motion correction in cryo-EM single-particle analysis Zivanov, Jasenko; Nakane, Takanori; Scheres, Sjors H. W. IUCrJ, Vol. 6, Issue 1 https://doi.org/10.1107/S205225251801463X	journal	January 2019
Real-space refinement in PHENIX for cryo-EM and crystallography Afonine, Pavel V.; Poon, Billy K.; Read, Randy J. Acta Crystallographica Section D Structural Biology, Vol. 74, Issue 6 https://doi.org/10.1107/S2059798318006551	journal	May 2018
New tools for the analysis and validation of cryo-EM maps and atomic models Afonine, Pavel V.; Klaholz, Bruno P.; Moriarty, Nigel W. Acta Crystallographica Section D Structural Biology, Vol. 74, Issue 9 https://doi.org/10.1107/S2059798318009324	journal	September 2018
The structure of INI 1/ hSNF 5 RPT 1 and its interactions with the c‐ MYC : MAX heterodimer provide insights into the interplay between MYC and the SWI / SNF chromatin remodeling complex Sammak, Susan; Allen, Mark D.; Hamdani, Najoua The FEBS Journal, Vol. 285, Issue 22 https://doi.org/10.1111/febs.14660	journal	October 2018
Mammalian SWI/SNF chromatin remodeling complexes and cancer: Mechanistic insights gained from human genomics Kadoch, Cigall; Crabtree, Gerald R. Science Advances, Vol. 1, Issue 5 https://doi.org/10.1126/sciadv.1500447	journal	June 2015
Stimulation of GAL4 derivative binding to nucleosomal DNA by the yeast SWI/SNF complex Cote, J.; Quinn, J.; Workman, J. Science, Vol. 265, Issue 5168 https://doi.org/10.1126/science.8016655	journal	July 1994
Structure of the RSC complex bound to the nucleosome Ye, Youpi; Wu, Hao; Chen, Kangjing Science, Vol. 366, Issue 6467 https://doi.org/10.1126/science.aay0033	journal	October 2019
Architecture of the SWI/SNF-Nucleosome Complex Dechassa, M. L.; Zhang, B.; Horowitz-Scherer, R. Molecular and Cellular Biology, Vol. 28, Issue 19 https://doi.org/10.1128/MCB.00693-08	journal	July 2008
RSC Mobilizes Nucleosomes To Improve Accessibility of Repair Machinery to the Damaged Chromatin Shim, E. Y.; Hong, S. J.; Oum, J. -H. Molecular and Cellular Biology, Vol. 27, Issue 5 https://doi.org/10.1128/MCB.01956-06	journal	December 2006
The Biology of Chromatin Remodeling Complexes Clapier, Cedric R.; Cairns, Bradley R. Annual Review of Biochemistry, Vol. 78, Issue 1 https://doi.org/10.1146/annurev.biochem.77.062706.153223	journal	June 2009
Reverse Genetic Analysis of the Yeast RSC Chromatin Remodeler Reveals a Role for RSC3 and SNF5 Homolog 1 in Ploidy Maintenance Campsteijn, Coen; Wijnands-Collin, Anne-Marie J.; Logie, Colin PLoS Genetics, Vol. 3, Issue 6 https://doi.org/10.1371/journal.pgen.0030092	journal	January 2007
Biochemical Profiling of Histone Binding Selectivity of the Yeast Bromodomain Family Zhang, Qiang; Chakravarty, Suvobrata; Ghersi, Dario PLoS ONE, Vol. 5, Issue 1 https://doi.org/10.1371/journal.pone.0008903	journal	January 2010
The RSC Chromatin Remodeling Complex Bears an Essential Fungal-Specific Protein Module With Broad Functional Roles Wilson, Boris; Erdjument-Bromage, Hediye; Tempst, Paul Genetics, Vol. 172, Issue 2 https://doi.org/10.1534/genetics.105.047589	journal	October 2005
A Library of Yeast Transcription Factor Motifs Reveals a Widespread Function for Rsc3 in Targeting Nucleosome Exclusion at Promoters T., Chan, Esther; Z., Ansari, Aseem; Desiree, Tillo, The University of North Carolina at Chapel Hill University Libraries https://doi.org/10.17615/dr7d-s563	text	January 2008
Collaboration gets the most out of software Morin, Andrew; Eisenbraun, Ben; Key, Jason eLife, Vol. 2 https://doi.org/10.7554/eLife.01456	journal	September 2013
Characterisation of molecular motions in cryo-EM single-particle data by multi-body refinement in RELION Nakane, Takanori; Kimanius, Dari; Lindahl, Erik eLife, Vol. 7 https://doi.org/10.7554/eLife.36861	journal	June 2018
New tools for automated high-resolution cryo-EM structure determination in RELION-3 Zivanov, Jasenko; Nakane, Takanori; Forsberg, Björn O. eLife, Vol. 7 https://doi.org/10.7554/eLife.42166	journal	November 2018

Similar Records

Dual engagement of the nucleosomal acidic patches is essential for deposition of histone H2A.Z by SWR1C

Journal Article · Tue May 28 20:00:00 EDT 2024 · eLife · OSTI ID:2471663

Baculoviruses and nucleosome management

Journal Article · Sat Feb 14 23:00:00 EST 2015 · Virology · OSTI ID:22470152

Three-dimensional structure of human chromatin accessibility complex hCHRAC by electron microscopy

Journal Article · Sun Nov 30 23:00:00 EST 2008 · Journal of Structural Biology · OSTI ID:1040040

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
chromatin
chromatin remodeling
cryo-em
structural biology and molecular biophysics
transcription

Architecture of the chromatin remodeler RSC and insights into its nucleosome engagement

Citation Formats

References (83)

Similar Records

Related Subjects