Contrasting Mixotrophic Lifestyles Reveal Different Ecological Niches in Two Closely Related Marine Protists

Wilken, Susanne; Choi, Chang Jae; Worden, Alexandra Z.

doi:10.1111/jpy.12920

Title: Contrasting Mixotrophic Lifestyles Reveal Different Ecological Niches in Two Closely Related Marine Protists

Journal Article · Fri Nov 01 00:00:00 EDT 2019 · Journal of Phycology

DOI:https://doi.org/10.1111/jpy.12920· OSTI ID:1599695

^[1]; Choi, Chang Jae ^[2];

^[2]

Monterey Bay Aquarium Research Institute 7700 Sandholdt Road Moss Landing California 95039 USA, Department of Freshwater and Marine Ecology Institute for Biodiversity and Ecosystem Dynamics University of Amsterdam Science Park 904 Amsterdam 1098 XH The Netherlands
Monterey Bay Aquarium Research Institute 7700 Sandholdt Road Moss Landing California 95039 USA, Ocean EcoSystems Biology Unit GEOMAR Helmholtz Centre for Ocean Research Düsternbrooker Weg 20 Kiel 24105 Germany

Many marine microbial eukaryotes combine photosynthetic with phagotrophic nutrition, but incomplete understanding of such mixotrophic protists, their functional diversity, and underlying physiological mechanisms limits the assessment and modeling of their roles in present and future ocean ecosystems. We developed an experimental system to study responses of mixotrophic protists to availability of living prey and light, and used it to characterize contrasting physiological strategies in two stramenopiles in the genus Ochromonas . We show that oceanic isolate CCMP 1393 is an obligate mixotroph, requiring both light and prey as complementary resources. Interdependence of photosynthesis and heterotrophy in CCMP 1393 comprises a significant role of mitochondrial respiration in photosynthetic electron transport. In contrast, coastal isolate CCMP 2951 is a facultative mixotroph that can substitute photosynthesis by phagotrophy and hence grow purely heterotrophically in darkness. In contrast to CCMP 1393, CCMP 2951 also exhibits a marked photoprotection response that integrates non‐photochemical quenching and mitochondrial respiration as electron sink for photosynthetically produced reducing equivalents. Facultative mixotrophs similar to CCMP 2951 might be well adapted to variable environments, while obligate mixotrophs similar to CCMP 1393 appear capable of resource efficient growth in oligotrophic ocean environments. Thus, the responses of these phylogenetically close protists to the availability of different resources reveals niche differentiation that influences impacts in food webs and leads to opposing carbon cycle roles.

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Research Organization:: Monterey Bay Aquarium Research Institute, Moss Landing, CA (United States)

Sponsoring Organization:: USDOE Office of Science (SC)

Grant/Contract Number:: DOE‐DE‐SC0004765; SC0004765

OSTI ID:: 1599695

Alternate ID(s):: OSTI ID: 1572734; OSTI ID: 1625906

Journal Information:: Journal of Phycology, Journal Name: Journal of Phycology Vol. 56 Journal Issue: 1; ISSN 0022-3646

Publisher:: Wiley-BlackwellCopyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 25 works

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