A genetic toolbox for metabolic engineering of Issatchenkia orientalis

Cao, Mingfeng; Fatma, Zia; Song, Xiaofei; Hsieh, Ping-Hung; Tran, Vinh G.; Lyon, William L.; Sayadi, Maryam; Shao, Zengyi; Yoshikuni, Yasuo; Zhao, Huimin

doi:10.1016/j.ymben.2020.01.005

A genetic toolbox for metabolic engineering of Issatchenkia orientalis

Journal Article · Fri Jan 31 00:00:00 EST 2020 · Metabolic Engineering

DOI:https://doi.org/10.1016/j.ymben.2020.01.005· OSTI ID:1599331

Cao, Mingfeng ^[1]; Fatma, Zia ^[2]; Song, Xiaofei ^[3]; Hsieh, Ping-Hung ^[4]; Tran, Vinh G. ^[2]; Lyon, William L. ^[2]; Sayadi, Maryam ^[5]; Shao, Zengyi ^[5]; Yoshikuni, Yasuo ^[4]; Zhao, Huimin ^[2]

Center for Advanced Bioenergy and Bioproducts Innovation (CABBI), Urbana, IL (United States); University of Illinois at Urbana-Champaign, IL (United States); CABBI
Center for Advanced Bioenergy and Bioproducts Innovation (CABBI), Urbana, IL (United States); University of Illinois at Urbana-Champaign, IL (United States)
Center for Advanced Bioenergy and Bioproducts Innovation (CABBI), Urbana, IL (United States); University of Illinois at Urbana-Champaign, IL (United States); Nankai University, Tianjin (China)
Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)
Iowa State University, Ames, IA (United States)

The nonconventional yeast Issatchenkia orientalis can grow under highly acidic conditions and has been explored for production of various organic acids. Yet, its broader application is hampered by the lack of efficient genetic tools to enable sophisticated metabolic manipulations. We recently constructed an episomal plasmid based on the autonomously replicating sequence (ARS) from Saccharomyces cerevisiae (ScARS) in I. orientalis and developed a CRISPR/Cas9 system for multiplexed gene deletions. Here we report three additional genetic tools including: (1) identification of a 0.8 kb centromere-like (CEN-L) sequence from the I. orientalis genome by using bioinformatics and functional screening; (2) discovery and characterization of a set of constitutive promoters and terminators under different culture conditions by using RNA-Seq analysis and a fluorescent reporter; and (3) development of a rapid and efficient in vivo DNA assembly method in I. orientalis, which exhibited ~100% fidelity when assembling a 7 kb-plasmid from seven DNA fragments ranging from 0.7 kb to 1.7 kb. As evidence of concept, we used these genetic tools to rapidly construct a functional xylose utilization pathway in I. orientalis.

View Accepted Manuscript (DOE)

Research Organization:: Center for Advanced Bioenergy and Bioproducts Innovation (CABBI), Urbana, IL (United States); University of California, Berkeley, CA (United States); University of Illinois at Urbana-Champaign, IL (United States)

Sponsoring Organization:: China Scholarship Council; National Science Foundation (NSF); USDOE Office of Science (SC), Biological and Environmental Research (BER); USDOE Office of Science (SC), Biological and Environmental Research (BER) (SC-23)

Grant/Contract Number:: SC0018420

OSTI ID:: 1599331

Alternate ID(s):: OSTI ID: 1599237
OSTI ID: 1991861

Journal Information:: Metabolic Engineering, Journal Name: Metabolic Engineering Journal Issue: C Vol. 59; ISSN 1096-7176

Publisher:: ElsevierCopyright Statement

Country of Publication:: United States

Language:: English

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59 BASIC BIOLOGICAL SCIENCES
Centromere-like sequence
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A genetic toolbox for metabolic engineering of Issatchenkia orientalis

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