Metalloprotein switches that display chemical-dependent electron transfer in cells

Atkinson, Joshua T.; Campbell, Ian J.; Thomas, Emily E.; Bonitatibus, Sheila C.; Elliott, Sean J.; Bennett, George N.; Silberg, Jonathan J.

doi:10.1038/s41589-018-0192-3

Title: Metalloprotein switches that display chemical-dependent electron transfer in cells

Journal Article · Mon Dec 17 00:00:00 EST 2018 · Nature Chemical Biology

DOI:https://doi.org/10.1038/s41589-018-0192-3· OSTI ID:1593987

^[1]; Campbell, Ian J. ^[1]; Thomas, Emily E. ^[1]; Bonitatibus, Sheila C. ^[2];

^[2]; Bennett, George N. ^[1];

^[1]

Rice Univ., Houston, TX (United States)
Boston Univ., MA (United States). Dept. of Chemistry

Biological electron transfer is challenging to directly regulate using environmental conditions. To enable dynamic, protein level control over energy flow in metabolic systems for synthetic biology and bioelectronics, we created ferredoxin logic gates that utilize transcriptional and post-translational inputs to control energy flow through a synthetic electron transfer pathway that is required for bacterial growth. These logic gates were created by subjecting a thermostable, plant-type ferredoxin to backbone fission and fusing the resulting fragments to a pair of proteins that self-associate, a pair of proteins whose association is stabilized by a small molecule, and to the termini of a ligand-binding domain. In our work we show that the latter domain insertion design strategy yields an allosteric ferredoxin switch that acquires an oxygen-tolerant [2Fe–2S] cluster and can use different chemicals, including a therapeutic drug and an environmental pollutant, to control the production of a reduced metabolite in Escherichia coli and cell lysates.

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Research Organization:: Rice Univ., Houston, TX (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Basic Energy Sciences (BES); National Aeronautics and Space Administration (NASA); US Department of the Navy, Office of Naval Research (ONR); National Science Foundation (NSF)

Grant/Contract Number:: SC0014462; NNX15AL28G; N00014-17-1-2639; SC0012598

OSTI ID:: 1593987

Journal Information:: Nature Chemical Biology, Vol. 15, Issue 2; ISSN 1552-4450

Publisher:: Nature Publishing GroupCopyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 31 works

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Title: Metalloprotein switches that display chemical-dependent electron transfer in cells

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