Automated mass spectrometry imaging of over 2000 proteins from tissue sections at 100-µm spatial resolution

Piehowski, Paul D.; Zhu, Ying; Bramer, Lisa M.; Stratton, Kelly G.; Zhao, Rui; Orton, Daniel J.; Moore, Ronald J.; Yuan, Jian; Mitchell, Hugh D.; Gao, Yuqian; Webb-Robertson, Bobbie-Jo M.; Dey, Sudhansu K.; Kelly, Ryan T.; Burnum-Johnson, Kristin E.

doi:10.1038/s41467-019-13858-z

Title: Automated mass spectrometry imaging of over 2000 proteins from tissue sections at 100-µm spatial resolution

Journal Article · Tue Jan 07 00:00:00 EST 2020 · Nature Communications

DOI:https://doi.org/10.1038/s41467-019-13858-z· OSTI ID:1592415

^[1];

^[1]; Zhao, Rui ^[1];

^[1]; Moore, Ronald J. ^[1]; Yuan, Jian ^[2]; Mitchell, Hugh D. ^[1]; Gao, Yuqian ^[1]; Webb-Robertson, Bobbie-Jo M. ^[1]; Dey, Sudhansu K. ^[3];

^[1];

^[1]

BATTELLE (PACIFIC NW LAB)
Cincinnati Children’s Hospital Medical Center
University of Cincinnati

Biological tissues exhibit complex spatial heterogeneity that directs the functions of multicellular organisms. Quantifying protein expression is essential for elucidating processes within complex biological assemblies. Imaging mass spectrometry (IMS) is a powerful emerging tool for mapping the spatial distribution of metabolites and lipids across tissue surfaces, but technical challenges have limited the application of IMS to the analysis of proteomes. Methods for probing the spatial distribution of the proteome have generally relied on the use of labels and/or antibodies, which limits multiplexing and requires a priori knowledge of protein targets. Past efforts to make spatially resolved proteome measurements across tissues have had limited spatial resolution and proteome coverage and have relied on manual workflows. Here, we demonstrate an automated approach to imaging that utilizes label-free nanoproteomics to analyze tissue voxels, generating quantitative cell-type-specific images for >2000 proteins with 100-µm spatial resolution across mouse uterine tissue sections preparing for blastocyst implantation.

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Research Organization:: Pacific Northwest National Laboratory (PNNL), Richland, WA (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER); National Institutes of Health (NIH); March of Dimes

DOE Contract Number:: AC05-76RLO1830; SC0019620; R21-HD084788; UG3HL145593; R33-CA225248; R01HD068524; DA006668; 22-FY17- 889

OSTI ID:: 1592415

Report Number(s):: PNNL-SA-138884

Journal Information:: Nature Communications, Vol. 11, Issue 1

Country of Publication:: United States

Language:: English

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