Alternative conformations of a major antigenic site on RSV F

Jones, Harrison G.; Battles, Michael B.; Lin, Chun-Chi; Bianchi, Siro; Corti, Davide; McLellan, Jason S.; Crowe, Jr., James E.

doi:10.1371/journal.ppat.1007944

Title: Alternative conformations of a major antigenic site on RSV F

Journal Article · Mon Jul 15 00:00:00 EDT 2019 · PLoS Pathogens

DOI:https://doi.org/10.1371/journal.ppat.1007944· OSTI ID:1591911

Jones, Harrison G. ^[1];

^[2]; Lin, Chun-Chi ^[3];

^[4]; Corti, Davide ^[4];

^[5]; Crowe, Jr., James E. ^[6]

Geisel School of Medicine at Dartmouth, Hanover, NH (United States); Univ. of Texas, Austin, TX (United States)
Geisel School of Medicine at Dartmouth, Hanover, NH (United States); Adimab LLC, Lebanon, NH (United States)
Geisel School of Medicine at Dartmouth, Hanover, NH (United States)
Humabs BioMed SA, Bellinzona (Switzerland)
Univ. of Texas, Austin, TX (United States)
Vanderbilt Univ., Nashville, TN (United States)

The respiratory syncytial virus (RSV) fusion (F) glycoprotein is a major target of neutralizing antibodies arising from natural infection, and antibodies that specifically bind to the prefusion conformation of RSV F generally demonstrate the greatest neutralization potency. Prefusion-stabilized RSV F variants have been engineered as vaccine antigens, but crystal structures of these variants have revealed conformational differences in a key antigenic site located at the apex of the trimer, referred to as antigenic site Ø. Currently, it is unclear if flexibility in this region is an inherent property of prefusion RSV F or if it is related to inadequate stabilization of site Ø in the engineered variants. Therefore, we set out to investigate the conformational flexibility of antigenic site Ø, as well as the ability of the human immune system to recognize alternative conformations of this site, by determining crystal structures of prefusion RSV F bound to neutralizing human-derived antibodies AM22 and RSD5. Both antibodies bound with high affinity and were specific for the prefusion conformation of RSV F. Crystal structures of the complexes revealed that the antibodies recognized distinct conformations of antigenic site Ø, each diverging at a conserved proline residue located in the middle of an α-helix. These data suggest that antigenic site Ø exists as an ensemble of conformations, with individual antibodies recognizing discrete states. Collectively, these results have implications for the refolding of pneumovirus and paramyxovirus fusion proteins and should inform development of prefusion-stabilized RSV F vaccine candidates.

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Research Organization:: Argonne National Laboratory (ANL), Argonne, IL (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER); NIGMS

Grant/Contract Number:: AC02-06CH11357; AC02-98CH10886; P20GM113132

OSTI ID:: 1591911

Journal Information:: PLoS Pathogens, Vol. 15, Issue 7; ISSN 1553-7374

Publisher:: Public Library of ScienceCopyright Statement

Country of Publication:: United States

Language:: ENGLISH

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Cited by: 15 works

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References (71)

Contribution of the Respiratory Syncytial Virus G Glycoprotein and Its Secreted and Membrane-Bound Forms to Virus Replication in Vitro and in Vivo Teng, Michael N.; Whitehead, Stephen S.; Collins, Peter L. Virology, Vol. 289, Issue 2 https://doi.org/10.1006/viro.2001.1138	journal	October 2001
Generation of stable monoclonal antibody–producing B cell receptor–positive human memory B cells by genetic programming Kwakkenbos, Mark J.; Diehl, Sean A.; Yasuda, Etsuko Nature Medicine, Vol. 16, Issue 1 https://doi.org/10.1038/nm.2071	journal	December 2009
Time and Out-of-Pocket Costs Associated with Respiratory Syncytial Virus Hospitalization of Infants Leader, Shelah; Yang, Harry; DeVincenzo, John Value in Health, Vol. 6, Issue 2 https://doi.org/10.1046/j.1524-4733.2003.00220.x	journal	March 2003
Discovery of a Prefusion Respiratory Syncytial Virus F-Specific Monoclonal Antibody That Provides Greater In Vivo Protection than the Murine Precursor of Palivizumab Zhao, Min; Zheng, Zi-Zheng; Chen, Man Journal of Virology, Vol. 91, Issue 15 https://doi.org/10.1128/JVI.00176-17	journal	May 2017
Overview of the CCP 4 suite and current developments Winn, Martyn D.; Ballard, Charles C.; Cowtan, Kevin D. Acta Crystallographica Section D Biological Crystallography, Vol. 67, Issue 4 https://doi.org/10.1107/S0907444910045749	journal	March 2011
Structure of HIV-1 gp120 V1/V2 domain with broadly neutralizing antibody PG9 McLellan, Jason S.; Pancera, Marie; Carrico, Chris Nature, Vol. 480, Issue 7377 https://doi.org/10.1038/nature10696	journal	November 2011
Efficient generation of monoclonal antibodies from single human B cells by single cell RT-PCR and expression vector cloning Tiller, Thomas; Meffre, Eric; Yurasov, Sergey Journal of Immunological Methods, Vol. 329, Issue 1-2 https://doi.org/10.1016/j.jim.2007.09.017	journal	January 2008
Cell Biology of Virus Entry Dimitrov, Dimiter S. Cell, Vol. 101, Issue 7 https://doi.org/10.1016/S0092-8674(00)80882-X	journal	June 2000
Infants Infected with Respiratory Syncytial Virus Generate Potent Neutralizing Antibodies that Lack Somatic Hypermutation Goodwin, Eileen; Gilman, Morgan S. A.; Wrapp, Daniel Immunity, Vol. 48, Issue 2 https://doi.org/10.1016/j.immuni.2018.01.005	journal	February 2018
Structure of RSV Fusion Glycoprotein Trimer Bound to a Prefusion-Specific Neutralizing Antibody McLellan, J. S.; Chen, M.; Leung, S. Science, Vol. 340, Issue 6136 https://doi.org/10.1126/science.1234914	journal	April 2013
Respiratory Syncytial Virus with the Fusion Protein as Its only Viral Glycoprotein Is Less Dependent on Cellular Glycosaminoglycans for Attachment than Complete Virus Techaarpornkul, Sunee; Collins, Peter L.; Peeples, Mark E. Virology, Vol. 294, Issue 2 https://doi.org/10.1006/viro.2001.1340	journal	March 2002
Structure-Based Design of a Fusion Glycoprotein Vaccine for Respiratory Syncytial Virus McLellan, J. S.; Chen, M.; Joyce, M. G. Science, Vol. 342, Issue 6158 https://doi.org/10.1126/science.1243283	journal	October 2013
Structure of an HIV gp120 envelope glycoprotein in complex with the CD4 receptor and a neutralizing human antibody Kwong, Peter D.; Wyatt, Richard; Robinson, James Nature, Vol. 393, Issue 6686 https://doi.org/10.1038/31405	journal	June 1998
Vaccine Induction of Antibodies against a Structurally Heterogeneous Site of Immune Pressure within HIV-1 Envelope Protein Variable Regions 1 and 2 Liao, Hua-Xin; Bonsignori, Mattia; Alam, S. Munir Immunity, Vol. 38, Issue 1 https://doi.org/10.1016/j.immuni.2012.11.011	journal	January 2013
Ongoing developments in RSV prophylaxis: a clinician’s analysis Rezaee, Fariba; Linfield, Debra T.; Harford, Terri J. Current Opinion in Virology, Vol. 24 https://doi.org/10.1016/j.coviro.2017.03.015	journal	June 2017
Cryo-EM structures of MERS-CoV and SARS-CoV spike glycoproteins reveal the dynamic receptor binding domains Yuan, Yuan; Cao, Duanfang; Zhang, Yanfang Nature Communications, Vol. 8, Issue 1 https://doi.org/10.1038/ncomms15092	journal	April 2017
Effect of Proteolytic Processing at Two Distinct Sites on Shape and Aggregation of an Anchorless Fusion Protein of Human Respiratory Syncytial Virus and Fate of the Intervening Segment Begoña Ruiz-Argüello, M.; González-Reyes, Luis; Calder, Leslie J. Virology, Vol. 298, Issue 2 https://doi.org/10.1006/viro.2002.1497	journal	July 2002
Specific Single or Double Proline Substitutions in the “Spring-loaded” Coiled-Coil Region of the Influenza Hemagglutinin Impair or Abolish Membrane Fusion Activity Qiao, Hui; Pelletier, Sandra L.; Hoffman, Lucas The Journal of Cell Biology, Vol. 141, Issue 6 https://doi.org/10.1083/jcb.141.6.1335	journal	June 1998
Therapeutic efficacy of a respiratory syncytial virus fusion inhibitor Roymans, Dirk; Alnajjar, Sarhad S.; Battles, Michael B. Nature Communications, Vol. 8, Issue 1 https://doi.org/10.1038/s41467-017-00170-x	journal	August 2017
Respiratory Syncytial Virus Attachment Glycoprotein Contribution to Infection Depends on the Specific Fusion Protein Meng, Jia; Hotard, Anne L.; Currier, Michael G. Journal of Virology, Vol. 90, Issue 1 https://doi.org/10.1128/JVI.02140-15	journal	October 2015
Molecular mechanism of respiratory syncytial virus fusion inhibitors Battles, Michael B.; Langedijk, Johannes P.; Furmanova-Hollenstein, Polina Nature Chemical Biology, Vol. 12, Issue 2 https://doi.org/10.1038/nchembio.1982	journal	December 2015
Characterization of a Prefusion-Specific Antibody That Recognizes a Quaternary, Cleavage-Dependent Epitope on the RSV Fusion Glycoprotein Gilman, Morgan S. A.; Moin, Syed M.; Mas, Vicente PLOS Pathogens, Vol. 11, Issue 7 https://doi.org/10.1371/journal.ppat.1005035	journal	July 2015
Cross-neutralization of four paramyxoviruses by a human monoclonal antibody Corti, Davide; Bianchi, Siro; Vanzetta, Fabrizia Nature, Vol. 501, Issue 7467 https://doi.org/10.1038/nature12442	journal	August 2013
Iterative structure-based improvement of a fusion-glycoprotein vaccine against RSV Joyce, M. Gordon; Zhang, Baoshan; Ou, Li Nature Structural & Molecular Biology, Vol. 23, Issue 9 https://doi.org/10.1038/nsmb.3267	journal	August 2016
Structural basis of respiratory syncytial virus neutralization by motavizumab McLellan, Jason S.; Chen, Man; Kim, Albert Nature Structural & Molecular Biology, Vol. 17, Issue 2 https://doi.org/10.1038/nsmb.1723	journal	January 2010
Structural basis of respiratory syncytial virus subtype-dependent neutralization by an antibody targeting the fusion glycoprotein Tian, Daiyin; Battles, Michael B.; Moin, Syed M. Nature Communications, Vol. 8, Issue 1 https://doi.org/10.1038/s41467-017-01858-w	journal	November 2017
Neutralizing antibodies against the preactive form of respiratory syncytial virus fusion protein offer unique possibilities for clinical intervention Magro, M.; Mas, V.; Chappell, K. Proceedings of the National Academy of Sciences, Vol. 109, Issue 8 https://doi.org/10.1073/pnas.1115941109	journal	February 2012
Inference of Macromolecular Assemblies from Crystalline State Krissinel, Evgeny; Henrick, Kim Journal of Molecular Biology, Vol. 372, Issue 3 https://doi.org/10.1016/j.jmb.2007.05.022	journal	September 2007
Soluble Respiratory Syncytial Virus Fusion Protein in the Fully Cleaved, Pretriggered State Is Triggered by Exposure to Low-Molarity Buffer Chaiwatpongsakorn, S.; Epand, R. F.; Collins, P. L. Journal of Virology, Vol. 85, Issue 8 https://doi.org/10.1128/JVI.01813-10	journal	February 2011
Respiratory Syncytial Virus: Infection, Detection, and New Options for Prevention and Treatment Griffiths, Cameron; Drews, Steven J.; Marchant, David J. Clinical Microbiology Reviews, Vol. 30, Issue 1 https://doi.org/10.1128/CMR.00010-16	journal	November 2016
Respiratory Syncytial Virus-Associated Mortality in Hospitalized Infants and Young Children Byington, C. L.; Wilkes, J.; Korgenski, K. PEDIATRICS, Vol. 135, Issue 1 https://doi.org/10.1542/peds.2014-2151	journal	December 2014
Rapid profiling of RSV antibody repertoires from the memory B cells of naturally infected adult donors Gilman, M. S. A.; Castellanos, C. A.; Chen, M. Science Immunology, Vol. 1, Issue 6 https://doi.org/10.1126/sciimmunol.aaj1879	journal	December 2016
Cleavage of the human respiratory syncytial virus fusion protein at two distinct sites is required for activation of membrane fusion Gonzalez-Reyes, L.; Ruiz-Arguello, M. B.; Garcia-Barreno, B. Proceedings of the National Academy of Sciences, Vol. 98, Issue 17 https://doi.org/10.1073/pnas.151098198	journal	August 2001
IMGT(R), the international ImMunoGeneTics information system(R) Lefranc, M. -P.; Giudicelli, V.; Ginestoux, C. Nucleic Acids Research, Vol. 37, Issue Database https://doi.org/10.1093/nar/gkn838	journal	January 2009
iMOSFLM : a new graphical interface for diffraction-image processing with MOSFLM Battye, T. Geoff G.; Kontogiannis, Luke; Johnson, Owen Acta Crystallographica Section D Biological Crystallography, Vol. 67, Issue 4 https://doi.org/10.1107/S0907444910048675	journal	March 2011
Structural basis for nonneutralizing antibody competition at antigenic site II of the respiratory syncytial virus fusion protein Mousa, Jarrod J.; Sauer, Marion F.; Sevy, Alexander M. Proceedings of the National Academy of Sciences, Vol. 113, Issue 44 https://doi.org/10.1073/pnas.1609449113	journal	October 2016
The respiratory syncytial virus vaccine landscape: lessons from the graveyard and promising candidates Mazur, Natalie I.; Higgins, Deborah; Nunes, Marta C. The Lancet Infectious Diseases, Vol. 18, Issue 10 https://doi.org/10.1016/S1473-3099(18)30292-5	journal	October 2018
Global, regional, and national disease burden estimates of acute lower respiratory infections due to respiratory syncytial virus in young children in 2015: a systematic review and modelling study Shi, Ting; McAllister, David A.; O'Brien, Katherine L. The Lancet, Vol. 390, Issue 10098 https://doi.org/10.1016/S0140-6736(17)30938-8	journal	September 2017
Blocking Intercellular Adhesion Molecule-1 on Human Epithelial Cells Decreases Respiratory Syncytial Virus Infection Behera, Aruna K.; Matsuse, Hiroto; Kumar, Mukesh Biochemical and Biophysical Research Communications, Vol. 280, Issue 1 https://doi.org/10.1006/bbrc.2000.4093	journal	January 2001
Potent single-domain antibodies that arrest respiratory syncytial virus fusion protein in its prefusion state Rossey, Iebe; Gilman, Morgan S. A.; Kabeche, Stephanie C. Nature Communications, Vol. 8, Issue 1 https://doi.org/10.1038/ncomms14158	journal	February 2017
Beyond Receptor Expression: The Influence of Receptor Conformation, Density, and Affinity in HIV-1 Infection Doms, Robert W. Virology, Vol. 276, Issue 2 https://doi.org/10.1006/viro.2000.0612	journal	October 2000
EGFR Interacts with the Fusion Protein of Respiratory Syncytial Virus Strain 2-20 and Mediates Infection and Mucin Expression Currier, Michael G.; Lee, Sujin; Stobart, Christopher C. PLOS Pathogens, Vol. 12, Issue 5 https://doi.org/10.1371/journal.ppat.1005622	journal	May 2016
Recombinant Vesicular Stomatitis Virus Expressing Respiratory Syncytial Virus (RSV) Glycoproteins: RSV Fusion Protein Can Mediate Infection and Cell Fusion Kahn, Jeffrey S.; Schnell, Matthias J.; Buonocore, Linda Virology, Vol. 254, Issue 1 https://doi.org/10.1006/viro.1998.9535	journal	February 1999
Immunogenicity and structures of a rationally designed prefusion MERS-CoV spike antigen Pallesen, Jesper; Wang, Nianshuang; Corbett, Kizzmekia S. Proceedings of the National Academy of Sciences, Vol. 114, Issue 35 https://doi.org/10.1073/pnas.1707304114	journal	August 2017
Identification of nucleolin as a cellular receptor for human respiratory syncytial virus Tayyari, Farnoosh; Marchant, David; Moraes, Theo J. Nature Medicine, Vol. 17, Issue 9 https://doi.org/10.1038/nm.2444	journal	August 2011
Structural characterization of the human respiratory syncytial virus fusion protein core Zhao, X.; Singh, M.; Malashkevich, V. N. Proceedings of the National Academy of Sciences, Vol. 97, Issue 26 https://doi.org/10.1073/pnas.260499197	journal	December 2000
PHENIX: a comprehensive Python-based system for macromolecular structure solution Adams, Paul D.; Afonine, Pavel V.; Bunkóczi, Gábor Acta Crystallographica Section D Biological Crystallography, Vol. 66, Issue 2, p. 213-221 https://doi.org/10.1107/S0907444909052925	journal	January 2010
Architecture of respiratory syncytial virus revealed by electron cryotomography Liljeroos, L.; Krzyzaniak, M. A.; Helenius, A. Proceedings of the National Academy of Sciences, Vol. 110, Issue 27 https://doi.org/10.1073/pnas.1309070110	journal	June 2013
Elevated temperature triggers human respiratory syncytial virus F protein six-helix bundle formation Yunus, Abdul S.; Jackson, Trent P.; Crisafi, Katherine Virology, Vol. 396, Issue 2 https://doi.org/10.1016/j.virol.2009.10.040	journal	January 2010
Pre-fusion structure of a human coronavirus spike protein Kirchdoerfer, Robert N.; Cottrell, Christopher A.; Wang, Nianshuang Nature, Vol. 531, Issue 7592 https://doi.org/10.1038/nature17200	journal	March 2016
Structure and immunogenicity of pre-fusion-stabilized human metapneumovirus F glycoprotein Battles, Michael B.; Más, Vicente; Olmedillas, Eduardo Nature Communications, Vol. 8, Issue 1 https://doi.org/10.1038/s41467-017-01708-9	journal	November 2017
Proteolytic Activation of Respiratory Syncytial Virus Fusion Protein: CLEAVAGE AT TWO FURIN CONSENSUS SEQUENCES Zimmer, Gert; Budz, Linda; Herrler, Georg Journal of Biological Chemistry, Vol. 276, Issue 34 https://doi.org/10.1074/jbc.M102633200	journal	June 2001
Prefusion F–specific antibodies determine the magnitude of RSV neutralizing activity in human sera Ngwuta, Joan O.; Chen, Man; Modjarrad, Kayvon Science Translational Medicine, Vol. 7, Issue 309 https://doi.org/10.1126/scitranslmed.aac4241	journal	October 2015
Effectiveness of Palivizumab in Preventing RSV Hospitalization in High Risk Children: A Real-World Perspective Homaira, Nusrat; Rawlinson, William; Snelling, Thomas L. International Journal of Pediatrics, Vol. 2014 https://doi.org/10.1155/2014/571609	journal	January 2014
Heptad-Repeat Regions of Respiratory Syncytial Virus F 1 Protein Form a Six-Membered Coiled-Coil Complex Lawless-Delmedico, Mary K.; Sista, Prakash; Sen, Ratna Biochemistry, Vol. 39, Issue 38 https://doi.org/10.1021/bi000471y	journal	September 2000
Phaser crystallographic software McCoy, Airlie J.; Grosse-Kunstleve, Ralf W.; Adams, Paul D. Journal of Applied Crystallography, Vol. 40, Issue 4 https://doi.org/10.1107/S0021889807021206	journal	July 2007
A highly stable prefusion RSV F vaccine derived from structural analysis of the fusion mechanism Krarup, Anders; Truan, Daphné; Furmanova-Hollenstein, Polina Nature Communications, Vol. 6, Issue 1 https://doi.org/10.1038/ncomms9143	journal	September 2015
Circulation of human coronaviruses OC43 and 229E in Córdoba, Argentina Ottogalli, María Emilia; Rodríguez, Pamela Elizabeth; Frutos, María Celia Archives of Virology, Vol. 166, Issue 3 https://doi.org/10.1007/s00705-020-04914-x	journal	January 2021
Cavin1 intrinsically disordered domains are essential for fuzzy electrostatic interactions and caveola formation Tillu, Vikas A.; Rae, James; Gao, Ya Nature Communications, Vol. 12, Issue 1 https://doi.org/10.1038/s41467-021-21035-4	journal	February 2021
Overview of the CCP4 suite and current developments. Winn, Martyn D.; Ballard, Charles C.; Cowtan, Kevin D. Apollo - University of Cambridge Repository https://doi.org/10.17863/cam.52322	text	January 2011
PHENIX: a comprehensive Python-based system for macromolecular structure solution. Adams, Paul D.; Afonine, Pavel V.; Bunkóczi, Gábor Apollo - University of Cambridge Repository https://doi.org/10.17863/cam.45787	text	January 2010
Risk of Primary Infection and Reinfection With Respiratory Syncytial Virus Glezen, W. Paul Archives of Pediatrics & Adolescent Medicine, Vol. 140, Issue 6 https://doi.org/10.1001/archpedi.1986.02140200053026	journal	June 1986
Cleavage of the respiratory syncytial virus fusion protein is required for its surface expression: role of furin Bolt, Gert; Pedersen, Lars Østergaard; Birkeslund, Helle Harder Virus Research, Vol. 68, Issue 1 https://doi.org/10.1016/s0168-1702(00)00149-0	journal	June 2000
Receptor binding and priming of the spike protein of SARS-CoV-2 for membrane fusion Benton, Donald J.; Wrobel, Antoni G.; Xu, Pengqi Nature, Vol. 588, Issue 7837 https://doi.org/10.1038/s41586-020-2772-0	journal	September 2020
Safety, Tolerability and Pharmacokinetics of MEDI8897, an Extended Half-life Single-dose Respiratory Syncytial Virus Prefusion F-targeting Monoclonal Antibody Administered as a Single Dose to Healthy Preterm Infants Domachowske, Joseph B.; Khan, Anis A.; Esser, Mark T. The Pediatric Infectious Disease Journal, Vol. 37, Issue 9 https://doi.org/10.1097/inf.0000000000001916	journal	January 2018
A highly potent extended half-life antibody as a potential RSV vaccine surrogate for all infants Zhu, Qing; McLellan, Jason S.; Kallewaard, Nicole L. Science Translational Medicine, Vol. 9, Issue 388 https://doi.org/10.1126/scitranslmed.aaj1928	journal	May 2017
Identification of Linear Heparin-Binding Peptides Derived from Human Respiratory Syncytial Virus Fusion Glycoprotein That Inhibit Infectivity Crim, R. L.; Audet, S. A.; Feldman, S. A. Journal of Virology, Vol. 81, Issue 1 https://doi.org/10.1128/jvi.01226-06	journal	October 2006
The Core of the Respiratory Syncytial Virus Fusion Protein Is a Trimeric Coiled Coil Matthews, J. M.; Young, T. F.; Tucker, S. P. Journal of Virology, Vol. 74, Issue 13 https://doi.org/10.1128/jvi.74.13.5911-5920.2000	journal	July 2000
The Fusion Glycoprotein of Human Respiratory Syncytial Virus Facilitates Virus Attachment and Infectivity via an Interaction with Cellular Heparan Sulfate Feldman, S. A.; Audet, S.; Beeler, J. A. Journal of Virology, Vol. 74, Issue 14 https://doi.org/10.1128/jvi.74.14.6442-6447.2000	journal	July 2000
Stabilization of the Soluble, Cleaved, Trimeric Form of the Envelope Glycoprotein Complex of Human Immunodeficiency Virus Type 1 Sanders, R. W.; Vesanen, M.; Schuelke, N. Journal of Virology, Vol. 76, Issue 17, p. 8875-8889 https://doi.org/10.1128/jvi.76.17.8875-8889.2002	journal	September 2002
Respiratory syncytial virus: current and emerging treatment options Turner, Tiffany; Kopp, Benjamin; Paul, Grace ClinicoEconomics and Outcomes Research https://doi.org/10.2147/ceor.s60710	journal	April 2014

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