Modeling Substrate Utilization, Metabolite Production, and Uranium Immobilization in Shewanella oneidensis Biofilms

Renslow, Ryan S.; Ahmed, Bulbul; Nunez, Jamie; Cao, Bin; Majors, Paul D.; Fredrickson, Jim K.; Beyenal, Haluk

doi:10.3389/fenvs.2017.00030

Title: Modeling Substrate Utilization, Metabolite Production, and Uranium Immobilization in Shewanella oneidensis Biofilms

Journal Article · Thu Jun 29 00:00:00 EDT 2017 · Frontiers in Environmental Science

DOI:https://doi.org/10.3389/fenvs.2017.00030· OSTI ID:1582421

^[1]; Ahmed, Bulbul ^[2]; Nunez, Jamie ^[3]; Cao, Bin ^[1]; Majors, Paul D. ^[3];

^[3]; Beyenal, Haluk ^[2]

Washington State Univ., Pullman, WA (United States); Pacific Northwest National Lab. (PNNL), Richland, WA (United States)
Washington State Univ., Pullman, WA (United States)
Pacific Northwest National Lab. (PNNL), Richland, WA (United States)

In this study, we developed a two-dimensional mathematical model to predict substrate utilization and metabolite production rates in Shewanella oneidensis MR-1 biofilm in the presence and absence of uranium (U). In our model, lactate and fumarate are used as the electron donor and the electron acceptor, respectively. The model includes the production of extracellular polymeric substances (EPS). The EPS bound to the cell surface and the EPS distributed in the biofilm were considered bound EPS (bEPS) and loosely associated EPS (laEPS), respectively. COMSOL^® Multiphysics finite element analysis software was used to solve the model numerically (model file provided in the Supplementary Material). The input variables of the model were the lactate and fumarate concentrations, cell density, EPS density, half saturation constant for fumarate, and diffusion coefficients of the substrates and metabolites. To estimate unknown parameters and calibrate the model, we used a custom designed biofilm reactor placed inside a nuclear magnetic resonance (NMR) microimaging and spectroscopy system and measured substrate utilization and metabolite production rates. From these data we estimated the yield coefficients, maximum substrate utilization rate, half saturation constant for lactate, stoichiometric ratio of fumarate and acetate to lactate and stoichiometric ratio of succinate to fumarate. These parameters are critical to predicting the activity of biofilms and are not available in the literature. Lastly, the model was used to predict uranium immobilization in S. oneidensis MR-1 biofilms by considering reduction and adsorption processes in the cells and in the EPS. We found that the majority of immobilization was due to cells, and that EPS were less efficient at immobilizing U. Furthermore, most of the immobilization occurred within the top 10 µm of the biofilm. To the best of our knowledge, this research is one of the first biofilm immobilization mathematical models based on experimental observation. It has the ability to predict the relative contributions to U immobilization of laEPS, bEPS and cells.

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Research Organization:: Pacific Northwest National Laboratory (PNNL), Richland, WA (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER)

Grant/Contract Number:: AC05-76RL01830; FG92-08ER64560

OSTI ID:: 1582421

Report Number(s):: PNNL-SA-123748; TRN: US2100909

Journal Information:: Frontiers in Environmental Science, Vol. 5; ISSN 2296-665X

Publisher:: Frontiers Research FoundationCopyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 8 works

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biofilm
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EPS
modeling
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substrate 14 utilization
uranium

Title: Modeling Substrate Utilization, Metabolite Production, and Uranium Immobilization in Shewanella oneidensis Biofilms

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