Comparative ultrafast spectroscopy and structural analysis of OCP1 and OCP2 from Tolypothrix

Kuznetsova, Valentyna; Dominguez-Martin, Maria Agustina; Bao, Han; Gupta, Sayan; Sutter, Markus; Kloz, Miroslav; Rebarz, Mateusz; Přeček, Martin; Chen, Yan; Petzold, Christopher J.; Ralston, Corie Y.; Kerfeld, Cheryl A.; Polívka, Tomáš

doi:10.1016/j.bbabio.2019.148120

Title: Comparative ultrafast spectroscopy and structural analysis of OCP1 and OCP2 from Tolypothrix

Journal Article · Sat Feb 01 00:00:00 EST 2020 · Biochimica et Biophysica Acta - Bioenergetics

DOI:https://doi.org/10.1016/j.bbabio.2019.148120· OSTI ID:1763819

Kuznetsova, Valentyna; Dominguez-Martin, Maria Agustina; Bao, Han; Gupta, Sayan; Sutter, Markus; Kloz, Miroslav; Rebarz, Mateusz; Přeček, Martin; Chen, Yan; Petzold, Christopher J.; Ralston, Corie Y.; Kerfeld, Cheryl A.; Polívka, Tomáš

The orange carotenoid protein (OCP) is a structurally and functionally modular photoactive protein involved in cyanobacterial photoprotection. Recently, based on bioinformatic analysis and phylogenetic relationships, new families of OCP have been described, OCP2 and OCPx. The first characterization of the OCP2 showed both faster photoconversion and back-conversion, and lower fluorescence quenching of phycobilisomes relative to the well-characterized OCP1. Moreover, OCP2 is not regulated by the fluorescence recovery protein (FRP). In this work, we present a comprehensive study combining ultrafast spectroscopy and structural analysis to compare the photoactivation mechanisms of OCP1 and OCP2 from Tolypothrix PCC 7601. We show that despite significant differences in their functional characteristics, the spectroscopic properties of OCP1 and OCP2 are comparable. This indicates that the OCP functionality is not directly related to the spectroscopic properties of the bound carotenoid. In addition, the structural analysis by X-ray footprinting reveals that, overall, OCP1 and OCP2 have grossly the same photoactivation mechanism. However, the OCP2 is less reactive to radiolytic labeling, suggesting that the protein is less flexible than OCP1. Finally, this observation could explain fast photoconversion of OCP2.

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Research Organization:: Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States); Michigan State Univ., East Lansing, MI (United States). MSU-DOE Plant Research Laboratory

Sponsoring Organization:: USDOE Office of Science (SC), Basic Energy Sciences (BES)

Grant/Contract Number:: FG02-91ER20021; AC02-05CH11231

OSTI ID:: 1763819

Alternate ID(s):: OSTI ID: 1575946; OSTI ID: 1581083; OSTI ID: 1734847; OSTI ID: 1735819

Journal Information:: Biochimica et Biophysica Acta - Bioenergetics, Journal Name: Biochimica et Biophysica Acta - Bioenergetics Vol. 1861 Journal Issue: 2; ISSN 0005-2728

Publisher:: ElsevierCopyright Statement

Country of Publication:: Netherlands

Language:: English

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Cited by: 13 works

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59 BASIC BIOLOGICAL SCIENCES
OCP1
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Photoactivation
X-ray footprinting
Ultrafast spectroscopy

Title: Comparative ultrafast spectroscopy and structural analysis of OCP1 and OCP2 from Tolypothrix

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