skip to main content
OSTI.GOV title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Recognition of the β-lactam carboxylate triggers acylation of Neisseria gonorrhoeae penicillin-binding protein 2

Authors:
; ; ORCiD logo; ORCiD logo
Publication Date:
Research Org.:
Argonne National Lab. (ANL), Argonne, IL (United States). Advanced Photon Source (APS)
Sponsoring Org.:
National Institutes of Health (NIH)
OSTI Identifier:
1577153
Resource Type:
Journal Article
Journal Name:
Journal of Biological Chemistry
Additional Journal Information:
Journal Volume: 294; Journal Issue: 38; Journal ID: ISSN 0021-9258
Publisher:
American Society for Biochemistry and Molecular Biology
Country of Publication:
United States
Language:
ENGLISH

Citation Formats

Singh, Avinash, Tomberg, Joshua, Nicholas, Robert A., and Davies, Christopher. Recognition of the β-lactam carboxylate triggers acylation of Neisseria gonorrhoeae penicillin-binding protein 2. United States: N. p., 2019. Web. doi:10.1074/jbc.RA119.009942.
Singh, Avinash, Tomberg, Joshua, Nicholas, Robert A., & Davies, Christopher. Recognition of the β-lactam carboxylate triggers acylation of Neisseria gonorrhoeae penicillin-binding protein 2. United States. doi:10.1074/jbc.RA119.009942.
Singh, Avinash, Tomberg, Joshua, Nicholas, Robert A., and Davies, Christopher. Tue . "Recognition of the β-lactam carboxylate triggers acylation of Neisseria gonorrhoeae penicillin-binding protein 2". United States. doi:10.1074/jbc.RA119.009942.
@article{osti_1577153,
title = {Recognition of the β-lactam carboxylate triggers acylation of Neisseria gonorrhoeae penicillin-binding protein 2},
author = {Singh, Avinash and Tomberg, Joshua and Nicholas, Robert A. and Davies, Christopher},
abstractNote = {},
doi = {10.1074/jbc.RA119.009942},
journal = {Journal of Biological Chemistry},
issn = {0021-9258},
number = 38,
volume = 294,
place = {United States},
year = {2019},
month = {7}
}

Works referenced in this record:

Neutral β-Lactams Inactivate High Molecular Mass Penicillin-Binding Proteins of Class B1, Including PBP2a of MRSA
journal, December 2013

  • Dave, Kinjal; Palzkill, Timothy; Pratt, R. F.
  • ACS Medicinal Chemistry Letters, Vol. 5, Issue 2
  • DOI: 10.1021/ml400408c

BOCILLIN FL, a Sensitive and Commercially Available Reagent for Detection of Penicillin-Binding Proteins
journal, May 1999

  • Zhao, Genshi; Meier, Timothy I.; Kahl, Steven D.
  • Antimicrobial Agents and Chemotherapy, Vol. 43, Issue 5
  • DOI: 10.1128/AAC.43.5.1124

Refinement of Macromolecular Structures by the Maximum-Likelihood Method
journal, May 1997

  • Murshudov, G. N.; Vagin, A. A.; Dodson, E. J.
  • Acta Crystallographica Section D Biological Crystallography, Vol. 53, Issue 3
  • DOI: 10.1107/S0907444996012255

Inactivation of Mycobacterium tuberculosis l,d-Transpeptidase Ldt Mt1 by Carbapenems and Cephalosporins
journal, May 2012

  • Dubée, Vincent; Triboulet, Sébastien; Mainardi, Jean-Luc
  • Antimicrobial Agents and Chemotherapy, Vol. 56, Issue 8
  • DOI: 10.1128/AAC.00665-12

Is Neisseria gonorrhoeae Initiating a Future Era of Untreatable Gonorrhea?: Detailed Characterization of the First Strain with High-Level Resistance to Ceftriaxone
journal, May 2011

  • Ohnishi, Makoto; Golparian, Daniel; Shimuta, Ken
  • Antimicrobial Agents and Chemotherapy, Vol. 55, Issue 7
  • DOI: 10.1128/AAC.00325-11

The penicillin-binding proteins: structure and role in peptidoglycan biosynthesis
journal, March 2008


Structural basis for effectiveness of siderophore-conjugated monocarbams against clinically relevant strains of Pseudomonas aeruginosa
journal, December 2010

  • Han, S.; Zaniewski, R. P.; Marr, E. S.
  • Proceedings of the National Academy of Sciences, Vol. 107, Issue 51
  • DOI: 10.1073/pnas.1013092107

PROCHECK: a program to check the stereochemical quality of protein structures
journal, April 1993

  • Laskowski, R. A.; MacArthur, M. W.; Moss, D. S.
  • Journal of Applied Crystallography, Vol. 26, Issue 2
  • DOI: 10.1107/S0021889892009944

Diversity of penA Alterations and Subtypes in Neisseria gonorrhoeae Strains from Sydney, Australia, That Are Less Susceptible to Ceftriaxone
journal, June 2007

  • Whiley, D. M.; Limnios, E. A.; Ray, S.
  • Antimicrobial Agents and Chemotherapy, Vol. 51, Issue 9
  • DOI: 10.1128/AAC.00306-07

Various penA mutations together with mtrR, porB and ponA mutations in Neisseria gonorrhoeae isolates with reduced susceptibility to cefixime or ceftriaxone
journal, January 2010

  • Lee, S. -G.; Lee, H.; Jeong, S. H.
  • Journal of Antimicrobial Chemotherapy, Vol. 65, Issue 4
  • DOI: 10.1093/jac/dkp505

Identification of Amino Acids Conferring High-Level Resistance to Expanded-Spectrum Cephalosporins in the penA Gene from Neisseria gonorrhoeae Strain H041
journal, April 2013

  • Tomberg, Joshua; Unemo, Magnus; Ohnishi, Makoto
  • Antimicrobial Agents and Chemotherapy, Vol. 57, Issue 7
  • DOI: 10.1128/AAC.00093-13

Hybrid penicillin-binding proteins in penicillin-resistant strains of Neisseria gonorrhoeae
journal, March 1988


The Perfect Penicillin? Inhibition of a Bacterial DD-Peptidase by Peptidoglycan-Mimetic β-Lactams
journal, July 2004

  • Josephine, Helen R.; Kumar, Ish; Pratt, R. F.
  • Journal of the American Chemical Society, Vol. 126, Issue 26
  • DOI: 10.1021/ja048850s

Crystal Structures of Penicillin-Binding Protein 3 from Pseudomonas aeruginosa: Comparison of Native and Antibiotic-Bound Forms
journal, January 2011

  • Sainsbury, Sarah; Bird, Louise; Rao, Vincenzo
  • Journal of Molecular Biology, Vol. 405, Issue 1
  • DOI: 10.1016/j.jmb.2010.10.024

Crystal structures of penicillin-binding protein 3 in complexes with azlocillin and cefoperazone in both acylated and deacylated forms
journal, January 2016

  • Ren, Jingshan; Nettleship, Joanne E.; Males, Alexandra
  • FEBS Letters, Vol. 590, Issue 2
  • DOI: 10.1002/1873-3468.12054

Ceftriaxone-Resistant Neisseria gonorrhoeae , Japan
journal, January 2011

  • Ohnishi, Makoto; Saika, Takeshi; Hoshina, Shinji
  • Emerging Infectious Diseases, Vol. 17, Issue 1
  • DOI: 10.3201/eid1701.100397

Crystal Structure of Cefditoren Complexed with Streptococcus pneumoniae Penicillin-Binding Protein 2X: Structural Basis for Its High Antimicrobial Activity
journal, August 2007

  • Yamada, M.; Watanabe, T.; Miyara, T.
  • Antimicrobial Agents and Chemotherapy, Vol. 51, Issue 11
  • DOI: 10.1128/AAC.00743-07

Structural Basis for E. coli Penicillin Binding Protein (PBP) 2 Inhibition, a Platform for Drug Design
journal, April 2019


The Crystal Structure of Phosphonate-Inhibited d -Ala- d -Ala Peptidase Reveals an Analogue of a Tetrahedral Transition State ,
journal, February 2003

  • Silvaggi, Nicholas R.; Anderson, John W.; Brinsmade, Shaun R.
  • Biochemistry, Vol. 42, Issue 5
  • DOI: 10.1021/bi0268955

Crystal Structure of Penicillin-binding Protein 1a (PBP1a) Reveals a Mutational Hotspot Implicated in β-Lactam Resistance in Streptococcus pneumoniae
journal, January 2006

  • Contreras-Martel, Carlos; Job, Viviana; Di Guilmi, Anne Marie
  • Journal of Molecular Biology, Vol. 355, Issue 4
  • DOI: 10.1016/j.jmb.2005.10.030

Multiple Antibiotic Resistance due to a Single Mutation in Neisseria gonorrhoeae
journal, September 1973


Common Alterations in PBP1a from Resistant Streptococcus pneumoniae Decrease Its Reactivity toward β-Lactams
journal, November 2007

  • Job, Viviana; Carapito, Raphaël; Vernet, Thierry
  • Journal of Biological Chemistry, Vol. 283, Issue 8
  • DOI: 10.1074/jbc.M706181200

Distinctive Attributes of β-Lactam Target Proteins in Acinetobacter baumannii Relevant to Development of New Antibiotics
journal, December 2011

  • Han, Seungil; Caspers, Nicole; Zaniewski, Richard P.
  • Journal of the American Chemical Society, Vol. 133, Issue 50
  • DOI: 10.1021/ja208835z

A PBP2x from a Clinical Isolate of Streptococcus pneumoniae Exhibits an Alternative Mechanism for Reduction of Susceptibility to β-Lactam Antibiotics
journal, January 2004

  • Pernot, Lucile; Chesnel, Laurent; Le Gouellec, Audrey
  • Journal of Biological Chemistry, Vol. 279, Issue 16
  • DOI: 10.1074/jbc.M313492200

The structures of penicillin-binding protein 4 (PBP4) and PBP5 from Enterococci provide structural insights into β-lactam resistance
journal, October 2018

  • Moon, Thomas M.; D'Andréa, Éverton D.; Lee, Christopher W.
  • Journal of Biological Chemistry, Vol. 293, Issue 48
  • DOI: 10.1074/jbc.RA118.006052

Features and development of Coot
journal, March 2010

  • Emsley, P.; Lohkamp, B.; Scott, W. G.
  • Acta Crystallographica Section D Biological Crystallography, Vol. 66, Issue 4
  • DOI: 10.1107/S0907444910007493

Genetic characterisation of Neisseria gonorrhoeae resistant to both ceftriaxone and azithromycin
journal, July 2018


Crystal Structure of the Actinomadura R39 DD-peptidase Reveals New Domains in Penicillin-binding Proteins
journal, June 2005

  • Sauvage, Eric; Herman, Raphaël; Petrella, Stephanie
  • Journal of Biological Chemistry, Vol. 280, Issue 35
  • DOI: 10.1074/jbc.M503271200

High-Level Cefixime- and Ceftriaxone-Resistant Neisseria gonorrhoeae in France: Novel penA Mosaic Allele in a Successful International Clone Causes Treatment Failure
journal, December 2011

  • Unemo, Magnus; Golparian, Daniel; Nicholas, Robert
  • Antimicrobial Agents and Chemotherapy, Vol. 56, Issue 3
  • DOI: 10.1128/AAC.05760-11

Gonococcal Resistance to β-Lactams and Tetracycline Involves Mutation in Loop 3 of the Porin Encoded at the penB Locus
journal, November 1998

  • Gill, M. J.; Simjee, S.; Al-Hattawi, K.
  • Antimicrobial Agents and Chemotherapy, Vol. 42, Issue 11
  • DOI: 10.1128/AAC.42.11.2799

Structural Effect of the Asp345a Insertion in Penicillin-Binding Protein 2 from Penicillin-Resistant Strains of Neisseria gonorrhoeae
journal, November 2014

  • Fedarovich, Alena; Cook, Edward; Tomberg, Joshua
  • Biochemistry, Vol. 53, Issue 48
  • DOI: 10.1021/bi5011317

The Role of the β5–α11 Loop in the Active-Site Dynamics of Acylated Penicillin-Binding Protein A from Mycobacterium tuberculosis
journal, May 2012

  • Fedarovich, Alena; Nicholas, Robert A.; Davies, Christopher
  • Journal of Molecular Biology, Vol. 418, Issue 5
  • DOI: 10.1016/j.jmb.2012.02.021

Structural and Kinetic Characterization of Diazabicyclooctanes as Dual Inhibitors of Both Serine-β-Lactamases and Penicillin-Binding Proteins
journal, January 2016


PBP Active Site Flexibility as the Key Mechanism for β-Lactam Resistance in Pneumococci
journal, April 2009

  • Contreras-Martel, Carlos; Dahout-Gonzalez, Cécile; Martins, Alexandre Dos Santos
  • Journal of Molecular Biology, Vol. 387, Issue 4
  • DOI: 10.1016/j.jmb.2009.02.024

NMRPipe: A multidimensional spectral processing system based on UNIX pipes
journal, November 1995

  • Delaglio, Frank; Grzesiek, Stephan; Vuister, GeertenW.
  • Journal of Biomolecular NMR, Vol. 6, Issue 3
  • DOI: 10.1007/BF00197809

Electronic structures of cephalosporins and penicillins. 9. Departure of a leaving group in cephalosporins
journal, July 1979

  • Boyd, Donald B.; Lunn, W. H. W.
  • Journal of Medicinal Chemistry, Vol. 22, Issue 7
  • DOI: 10.1021/jm00193a006

Penicillin-Binding Proteins of Gram-Negative Bacteria
journal, July 1988


Penicillin Binding Proteins: key players in bacterial cell cycle and drug resistance processes
journal, September 2006


Analysis of amino acid sequences of penicillin-binding protein 2 in clinical isolates of Neisseria gonorrhoeae with reduced susceptibility to cefixime and ceftriaxone
journal, January 2008

  • Osaka, Kazuyoshi; Takahata, Masahiro; Takakura, Tadakazu
  • Journal of Infection and Chemotherapy, Vol. 14, Issue 3
  • DOI: 10.1007/s10156-008-0610-7

Molecular characterization of two high-level ceftriaxone-resistant Neisseria gonorrhoeae isolates detected in Catalonia, Spain
journal, May 2012

  • Camara, J.; Serra, J.; Ayats, J.
  • Journal of Antimicrobial Chemotherapy, Vol. 67, Issue 8
  • DOI: 10.1093/jac/dks162

Development of New Drugs for an Old Target — The Penicillin Binding Proteins
journal, October 2012


Phaser crystallographic software
journal, July 2007

  • McCoy, Airlie J.; Grosse-Kunstleve, Ralf W.; Adams, Paul D.
  • Journal of Applied Crystallography, Vol. 40, Issue 4
  • DOI: 10.1107/S0021889807021206

Structural Insights into the Anti-methicillin-resistant Staphylococcus aureus (MRSA) Activity of Ceftobiprole
journal, July 2012

  • Lovering, Andrew L.; Gretes, Michael C.; Safadi, Susan S.
  • Journal of Biological Chemistry, Vol. 287, Issue 38
  • DOI: 10.1074/jbc.M112.355644