A Handheld Medical Diagnostic Device for Harsh Environments
- Lawrence Livermore National Laboratory (LLNL), Livermore, CA (United States)
Medical diagnostic capabilities for extreme environments, are currently limited in nature to single marker type assays. We propose to develop a handheld device for medical diagnostics in extreme environments that provide sensitive and specific detection of biomarkers with an emphasis initially on radiation exposure. Such a device will have multiple uses, and will be easily adaptable to many applications. For example, first responders, the battlefield, in low resource settings in developing countries facing emerging disease outbreaks, and during long-duration space flights. To this end, we will develop highly adaptable microfluidic devices (cards) for profiling different biomarkers with an emphasis on those related to radiation exposure. We will leverage the previous centrifugal separator work from LLNL and Sandia National Laboratory. The radiation exposure biomarker work at LLNL will provide a biological platform for demonstrating the utility of the device. We will develop a fluidic system using a pump-less mechanical method (centrifugal) to move, control, order and layer fluids in the platform. This decreases complexity, while enabling simultaneous cell separation and plasma generation for two uses: the first is differential blood cell counts and the second is biomarker assay panels. This work will be accomplished in three Aims: Aim 1) Test multiplex microfluidic cards and integrated reader. Aim 2) Demonstrate system capabilities to take and process blood samples for multi-endpoint analysis. Aim 3) Test multiplex detection based on measuring differential blood cell counts, while measuring plasma protein biomarkers. The proposed work addresses the mission research challenge of chemical and biological countermeasure in that our work aims to provide new research capabilities to respond to man-made or natural outbreaks of pandemic diseases in particular in low resource and triage environments.
- Research Organization:
- Lawrence Livermore National Laboratory (LLNL), Livermore, CA (United States)
- Sponsoring Organization:
- USDOE National Nuclear Security Administration (NNSA)
- DOE Contract Number:
- AC52-07NA27344
- OSTI ID:
- 1574630
- Report Number(s):
- LLNL-TR-797245; 998969
- Country of Publication:
- United States
- Language:
- English
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