Imaging Macromolecular Structural Dynamics with Low-Dose, Time-Resolved Transmission Electron Microscopy

Technical Report · Tue Oct 29 04:00:00 EDT 2019

DOI:https://doi.org/10.2172/1572620· OSTI ID:1572620

McKeown, Joseph ^[1]; Segelke, Brent ^[1]; Coleman, Matthew ^[1]; Roehling, John ^[1]; Shelby, Megan ^[1]

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1. Lawrence Livermore National Laboratory (LLNL), Livermore, CA (United States)

All well-established experimental methods for investigating the structure of biological macromolecules have spatial and/or temporal limitations inherent to the technique that limit their applicability to studying macromolecular dynamics. The ability to measure macromolecular dynamics is key to gaining predictive mastery of biological systems. This work demonstrates that sufficient data can be acquired from low-Z soft materials using dynamic transmission electron microscopy (DTEM) to potentially allow measurements of macromolecular motion. While a dynamic measurement of macromolecular motion or structural change requires further effort, it is evident from this work that this is feasible under specific experimental conditions that can be achieved using low-dose, time-resolved transmission electron microscopy.

• Research Organization: Lawrence Livermore National Laboratory (LLNL), Livermore, CA (United States)
• Sponsoring Organization: USDOE National Nuclear Security Administration (NNSA)
• DOE Contract Number: AC52-07NA27344
• OSTI ID: 1572620
• Report Number(s): LLNL--TR-795619; 996947
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES