Engineering Saccharomyces cerevisiae for co-utilization of d-galacturonic acid and d-glucose from citrus peel waste

Protzko, Ryan J.; Latimer, Luke N.; Martinho, Ze; de Reus, Elise; Seibert, Tanja; Benz, J. Philipp; Dueber, John E.

doi:10.1038/s41467-018-07589-w

Title: Engineering Saccharomyces cerevisiae for co-utilization of d-galacturonic acid and d-glucose from citrus peel waste

Journal Article · Thu Nov 29 00:00:00 EST 2018 · Nature Communications

DOI:https://doi.org/10.1038/s41467-018-07589-w· OSTI ID:1571943

Protzko, Ryan J. ^[1]; Latimer, Luke N. ^[2]; Martinho, Ze ^[3]; de Reus, Elise ^[4]; Seibert, Tanja ^[5];

^[6];

^[7]

Univ. of California, Berkeley, CA (United States). Dept. of Molecular and Cell Biology
Univ. of California, Berkeley, CA (United States). Dept. of Chemistry
Univ. of California, Berkeley, CA (United States). Dept. of Bioengineering
Technical Univ. of Denmark, Lyngby (Denmark). Dept. of Biotechnology and Biomedicine
Technische Univ. München, Freising (Germany); Max Planck Inst. of Molecular Plant Physiology, Potsdam, (Germany)
Technische Univ. München, Freising (Germany)
Univ. of California, Berkeley, CA (United States). Dept. of Bioengineering; Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States). Biological Systems & Engineering Division

Pectin-rich biomasses, such as citrus peel and sugar beet pulp, hold promise as inexpensive feedstocks for microbial fermentations as enzymatic hydrolysis of their component polysaccharides can be accomplished inexpensively to yield high concentrations of fermentable sugars and D-galacturonic acid (D-galUA). In this study, we tackle a number of challenges associated with engineering a microbial strain to convert pectin-rich hydrolysates into commodity and specialty chemicals. First, we engineer D-galUA utilization into yeast, Saccharomyces cerevisiae. Second, we identify that the mechanism of D-galUA uptake into yeast is mediated by hexose transporters and that consumption of D-galUA is inhibited by D-glucose. Third, we enable co-utilization of D-galUA and D-glucose by identifying and expressing a heterologous transporter, GatA, from Aspergillus niger. Last, we demonstrate the use of this transporter for production of the platform chemical, meso-galactaric acid, directly from industrial Navel orange peel waste.

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Research Organization:: Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)

Sponsoring Organization:: USDOE Office of Science (SC)

Grant/Contract Number:: AC02-05CH11231

OSTI ID:: 1571943

Journal Information:: Nature Communications, Vol. 9, Issue 1; ISSN 2041-1723

Publisher:: Nature Publishing GroupCopyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 50 works

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