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Preconditioning in the Rhesus Macaque Induces a Proteomic Signature Following Cerebral Ischemia that Is Associated with Neuroprotection

Journal Article · · Translational Stroke Research
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  1. Oregon Health & Science Univ., Portland, OR (United States)
  2. Pacific Northwest National Lab. (PNNL), Richland, WA (United States)
  3. Houston Methodist Neurosurgery, Houston, TX (United States)
  4. Oregon National Primate Research Center, Beaverton, OR (United States)
+ Show Author Affiliations
Each year thousands of patients are at risk of cerebral ischemic injury, due to iatrogenic responses to surgical procedures. Prophylactic treatment of these patients as standard care could minimize potential neurological complications. We have shown that protection of brain tissue, in a non-human primate model of cerebral ischemic injury, is possible through pharmacological preconditioning using the immune activator D192935. We postulate that preconditioning with D192935 results in neuroprotective reprogramming that is evident in the brain following experimentally induced cerebral ischemia. We performed quantitative proteomic analysis of cerebral spinal fluid (CSF) collected post-stroke from our previously published efficacy study to determine whether CSF protein profiles correlated with induced protection. Four groups of animals were examined: naïve animals (no treatment or stroke); animals treated with vehicle prior to stroke; D192935 treated and stroked animals, further delineated into two groups, ones that were protected (small infarcts) and those that were not protected (large infarcts). We found that distinct protein clusters defined the protected and non-protected animal groups, with a 16-member cluster of proteins induced exclusively in D192935 protected animals. Seventy percent of the proteins induced in the protected animals have functions that would enhance neuroprotection and tissue repair, including several members associated with M2 macrophages, a macrophage phenotype shown to contribute to neuroprotection and repair during ischemic injury. Overall, these studies highlight the translational importance of CSF biomarkers in defining mechanism and monitoring responses to treatment in development of stroke therapeutics.
Research Organization:
Pacific Northwest National Laboratory (PNNL), Richland, WA (United States). Environmental Molecular Sciences Laboratory (EMSL)
Sponsoring Organization:
National Institutes of Health (NIH); USDOE
Grant/Contract Number:
AC05-76RL01830
OSTI ID:
1571272
Report Number(s):
PNNL-SA--153569; PNWD-SA--10567
Journal Information:
Translational Stroke Research, Journal Name: Translational Stroke Research Journal Issue: 4 Vol. 10; ISSN 1868-4483
Publisher:
Springer Science+Business Media, LLCCopyright Statement
Country of Publication:
United States
Language:
English

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Cited By (2)

The role of microglia in ischemic preconditioning journal August 2019
Proteomic-Based Approaches for the Study of Ischemic Stroke journal July 2019

Figures / Tables (1)

Table 1(p. 13)table Table 1

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Related Subjects

60 APPLIED LIFE SCIENCES
cerebral spinal fluid
neuroprotection
non-human primates
proteomics
stroke