Skip to main content
U.S. Department of Energy
Office of Scientific and Technical Information

A compendium of multi-omic sequence information from the Saanich Inlet water column

Journal Article · · Scientific Data

Marine oxygen minimum zones (OMZs) are widespread regions of the ocean that are currently expanding due to global warming. While inhospitable to most metazoans, OMZs are hotspots for microbial mediated biogeochemical cycling of carbon, nitrogen and sulphur, contributing disproportionately to marine nitrogen loss and climate active trace gas production. Our current understanding of microbial community responses to OMZ expansion is limited by a lack of time-resolved data sets linking multi-omic sequence information (DNA, RNA, protein) to geochemical parameters and process rates. Here, we present six years of time-resolved multi-omic observations in Saanich Inlet, a seasonally anoxic fjord on the coast of Vancouver Island, British Columbia, Canada that undergoes recurring changes in water column oxygenation status. This compendium provides a unique multi-omic framework for studying microbial community responses to ocean deoxygenation along defined geochemical gradients in OMZ waters.

Research Organization:
Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)
Sponsoring Organization:
USDOE Office of Science (SC), Biological and Environmental Research (BER) (SC-23), Biological Systems Science Division (SC-23.2 )
DOE Contract Number:
AC02-05CH11231
OSTI ID:
1571089
Journal Information:
Scientific Data, Vol. 4, Issue 1; ISSN 2052-4463
Publisher:
Nature Publishing Group
Country of Publication:
United States
Language:
English

References (56)

Microbial oceanography of anoxic oxygen minimum zones journal September 2012
Microbial ecology of expanding oxygen minimum zones journal May 2012
Oxygen minimum zones (OMZs) in the modern ocean journal March 2009
A Cryptic Sulfur Cycle in Oxygen-Minimum-Zone Waters off the Chilean Coast journal November 2010
Revising the nitrogen cycle in the Peruvian oxygen minimum zone journal March 2009
Denitrification as the dominant nitrogen loss process in the Arabian Sea journal September 2009
Microbial Nitrogen Cycling Processes in Oxygen Minimum Zones journal January 2011
Methanotrophic Community Dynamics in a Seasonally Anoxic Fjord: Saanich Inlet, British Columbia journal December 2016
Marine hypoxia/anoxia as a source of CH 4 and N 2 O journal January 2010
Spreading Dead Zones and Consequences for Marine Ecosystems journal August 2008
Ocean Deoxygenation in a Warming World journal January 2010
Marine microorganisms and global nutrient cycles journal September 2004
Expanding Oxygen-Minimum Zones in the Tropical Oceans journal May 2008
Persistently declining oxygen levels in the interior waters of the eastern subarctic Pacific journal October 2007
Marine microbial community dynamics and their ecological interpretation journal February 2015
The information science of microbial ecology journal June 2016
Ironing out the wrinkles in the rare biosphere through improved OTU clustering: Ironing out the wrinkles in the rare biosphere journal March 2010
Sizing up metatranscriptomics journal August 2012
Microbial metatranscriptomics in a permanent marine oxygen minimum zone: OMZ community gene expression journal January 2011
Metaproteomics reveals differential modes of metabolic coupling among ubiquitous oxygen minimum zone microbes journal July 2014
Diverse Marinimicrobia bacteria may mediate coupled biogeochemical cycles along eco-thermodynamic gradients journal November 2017
Integrating biogeochemistry with multiomic sequence information in a model oxygen minimum zone journal September 2016
The oxygen minimum zone of the eastern South Pacific journal July 2009
Community Genomics Among Stratified Microbial Assemblages in the Ocean's Interior journal January 2006
Metatranscriptomics reveals unique microbial small RNAs in the ocean’s water column journal May 2009
Community Proteomics of a Natural Microbial Biofilm journal June 2005
Microbial community dynamics in a seasonally anoxic fjord: Saanich Inlet, British Columbia journal January 2010
Metagenome of a Versatile Chemolithoautotroph from Expanding Oceanic Dead Zones journal October 2009
Deep water renewal in Saanich Inlet, an intermittently anoxic basin journal January 1973
Oceanography of Saanich Inlet in Vancouver Island, British Columbia journal January 1962
SAR11 bacteria linked to ocean anoxia and nitrogen loss journal August 2016
A compendium of geochemical information from the Saanich Inlet water column journal October 2017
Ocean deoxygenation: Past, present, and future journal November 2011
Small Volume (1-3l) Filtration of Coastal Seawater Samples journal January 2009
Large Volume (20L+) Filtration of Coastal Seawater Samples journal January 2009
DNA Extraction from 0.22 μM Sterivex Filters and Cesium Chloride Density Gradient Centrifugation journal January 2009
Molecular Tools for Investigating Microbial Community Structure and Function in Oxygen-Deficient Marine Waters book January 2013
Every base matters: assessing small subunit rRNA primers for marine microbiomes with mock communities, time series and global field samples: Primers for marine microbiome studies journal October 2015
Diversity and population structure of Marine Group A bacteria in the Northeast subarctic Pacific Ocean journal November 2012
Seasonal and interannual variability of the marine bacterioplankton community throughout the water column over ten years journal September 2014
Major gradients in putatively nitrifying and non-nitrifying Archaea in the deep North Atlantic journal November 2008
Illumina GA IIx& HiSeq 2000 Production Sequenccing and QC Analysis Pipelines at the DOE Joint Genome Institute report January 2011
Extraordinary phylogenetic diversity and metabolic versatility in aquifer sediment journal August 2013
Genomic properties of Marine Group A bacteria indicate a role in the marine sulfur cycle journal September 2013
The Generating Function of CID, ETD, and CID/ETD Pairs of Tandem Mass Spectra: Applications to Database Search journal September 2010
Ecology and evolution of viruses infecting uncultivated SUP05 bacteria as revealed by single-cell- and meta-genomics journal August 2014
2016 update of the PRIDE database and its related tools journal November 2015
QIIME allows analysis of high-throughput community sequencing data journal April 2010
Greengenes, a Chimera-Checked 16S rRNA Gene Database and Workbench Compatible with ARB journal July 2006
SILVA: a comprehensive online resource for quality checked and aligned ribosomal RNA sequence data compatible with ARB journal November 2007
MetaPathways: a modular pipeline for constructing pathway/genome databases from environmental sequence information journal January 2013
New twist on nitrogen cycling in oceanic oxygen minimum zones journal March 2009
MetaPathways: a modular pipeline for constructing pathway/genome databases from environmental sequence information text January 2013
Primer and platform effects on 16S rRNA tag sequencing text January 2015
Combining genomic sequencing methods to explore viral diversity and reveal potential virus-host interactions journal April 2015
Gene swapping in the dead zone journal October 2014

Cited By (6)

Metabolite composition of sinking particles differs from surface suspended particles across a latitudinal transect in the South Atlantic journal July 2019
Diverse Marinimicrobia bacteria may mediate coupled biogeochemical cycles along eco-thermodynamic gradients journal November 2017
Monitoring microbial responses to ocean deoxygenation in a model oxygen minimum zone journal October 2017
Metabolite composition of sinking particles reflects a changing microbial community and differential metabolite degradation posted_content November 2018
A DNA barcode reference library of the French Polynesian shore fishes posted_content July 2019
Reconciling Dimensional and Categorical Models of Autism Heterogeneity: a Brain Connectomics & Behavioral Study posted_content July 2019