Skip to main content
OSTI.GOVU.S. Department of Energy Office of Scientific and Technical Information
U.S. Department of Energy
Office of Scientific and Technical Information
 

Imaging effectiveness calculator for non-design microscope samples

Journal Article · · Applied Optics
DOI:https://doi.org/10.1364/AO.58.006027· OSTI ID:1570285
 [1];  [1];  [1];  [1]
  1. Sandia National Lab. (SNL-NM), Albuquerque, NM (United States)
+ Show Author Affiliations
When attempting to integrate single-molecule fluorescence microscopy with microfabricated devices such as microfluidic channels, fabrication constraints may prevent using traditional coverslips. Instead, the fabricated devices may require imaging through material with a different thickness or index of refraction. Altering either can easily reduce the quality of the image formation (measured by the Strehl ratio) by a factor of 2 or more, reducing the signal-to-noise ratio accordingly. In such cases, successful detection of single-molecule fluorescence may prove difficult or impossible. Furthermore, we provide software to calculate the effect of non-design materials upon the Strehl ratio or ensquared energy and explore the impact of common materials used in microfabrication.
Research Organization:
Sandia National Laboratories (SNL-NM), Albuquerque, NM (United States)
Sponsoring Organization:
USDOE; USDOE National Nuclear Security Administration (NNSA)
Grant/Contract Number:
AC04-94AL85000; NA0003525
OSTI ID:
1570285
Alternate ID(s):
OSTI ID: 1544590
Report Number(s):
SAND--2019-11613J; 679980
Journal Information:
Applied Optics, Journal Name: Applied Optics Journal Issue: 22 Vol. 58; ISSN 1559-128X; ISSN APOPAI
Publisher:
Optical Society of AmericaCopyright Statement
Country of Publication:
United States
Language:
English

References (27)

Single-molecule fluorescence detection in microfluidic channels—the Holy Grail in μTAS? journal June 2005
Bio-assay based on single molecule fluorescence detection in microfluidic channels journal June 2006
Identification of acquisition parameters from the point spread function of a fluorescence microscope journal September 2001
Focusing of light through a stratified medium: a practical approach for computing microscope point spread functions. Part I: Conventional microscopy journal February 2003
Focusing of light through a stratified medium: a practical approach for computing microscope point spread functions journal May 2004
Determining refractive index of single living cell using an integrated microchip journal February 2007
Forbidden Light Detection from Single Molecules journal May 2000
The origins and the future of microfluidics journal July 2006
Sub-diffraction-limit imaging by stochastic optical reconstruction microscopy (STORM) journal August 2006
Efficient calculation of electromagnetic diffraction in optical systems using a multipole expansion journal April 1997
Fabrication of multilayer systems combining microfluidic and microoptical elements for fluorescence detection journal January 2001
Quantitative Comparison of Spot Detection Methods in Fluorescence Microscopy journal February 2010
3-D PSF fitting for fluorescence microscopy: implementation and localization application: 3-D PSF FITTING FOR FLUORESCENCE MICROSCOPY journal November 2012
Fluorescence microscopy point spread function model accounting for aberrations due to refractive index variability within a specimen journal July 2015
Highly efficient optical detection of surface-generated fluorescence conference April 1999
Highly efficient optical detection of surface-generated fluorescence journal January 1999
Light emission by magnetic and electric dipoles close to a plane interface I Total radiated power journal January 1977
Light emission by magnetic and electric dipoles close to a plane dielectric interface II Radiation patterns of perpendicular oriented dipoles journal January 1977
Light emission by magnetic and electric dipoles close to a plane dielectric interface III Radiation patterns of dipoles with arbitrary orientation journal January 1979
Allowed and forbidden light in near-field optics I A single dipolar light source journal January 1997
Allowed and forbidden light in near-field optics II Interacting dipolar particles journal January 1997
Fast and accurate three-dimensional point spread function computation for fluorescence microscopy journal January 2017
Experimental test of an analytical model of aberration in an oil-immersion objective lens used in three-dimensional light microscopy journal January 1992
Point spread function of optical microscopes imaging through stratified media journal January 2003
Full-field supercritical angle fluorescence microscopy for live cell imaging journal January 2011
Experimental test of an analytical model of aberration in an oil-immersion objective lens used in three-dimensional light microscopy journal January 1991
Full Field Supercritical Angle Fluorescence Microscopy for live cell imaging text January 2013

Figures / Tables (11)

Table 1(p. 4)table Table 1
Fig. 1(p. 5)figure Fig. 1
Table 2(p. 7)table Table 2
Fig. 2(p. 8)figure Fig. 2
Table 3(p. 8)table Table 3
Fig. 3(p. 9)figure Fig. 3
Fig. 4(p. 10)figure Fig. 4
Fig. 5(p. 11)figure Fig. 5
Fig. 6(p. 13)figure Fig. 6
Fig. 7(p. 14)figure Fig. 7
Fig. 8(p. 15)figure Fig. 8

Similar Records

Sandia Strehl Calculator Version 1.0
Software · Tue Dec 05 00:00:00 EST 2017 · OSTI ID:1418001

Related Subjects

47 OTHER INSTRUMENTATION