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Title: Imaging effectiveness calculator for non-design microscope samples

Journal Article · · Applied Optics
DOI:https://doi.org/10.1364/AO.58.006027· OSTI ID:1570285
ORCiD logo [1];  [1]; ORCiD logo [1];  [1]
  1. Sandia National Lab. (SNL-NM), Albuquerque, NM (United States)
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When attempting to integrate single-molecule fluorescence microscopy with microfabricated devices such as microfluidic channels, fabrication constraints may prevent using traditional coverslips. Instead, the fabricated devices may require imaging through material with a different thickness or index of refraction. Altering either can easily reduce the quality of the image formation (measured by the Strehl ratio) by a factor of 2 or more, reducing the signal-to-noise ratio accordingly. In such cases, successful detection of single-molecule fluorescence may prove difficult or impossible. Furthermore, we provide software to calculate the effect of non-design materials upon the Strehl ratio or ensquared energy and explore the impact of common materials used in microfabrication.

Research Organization:
Sandia National Lab. (SNL-NM), Albuquerque, NM (United States)
Sponsoring Organization:
USDOE National Nuclear Security Administration (NNSA)
Grant/Contract Number:
AC04-94AL85000; NA0003525
OSTI ID:
1570285
Alternate ID(s):
OSTI ID: 1544590
Report Number(s):
SAND-2019-11613J; APOPAI; 679980
Journal Information:
Applied Optics, Vol. 58, Issue 22; ISSN 1559-128X
Publisher:
Optical Society of AmericaCopyright Statement
Country of Publication:
United States
Language:
English

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Linked Research (2)

Figures / Tables (11)

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