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RpoN (σ54) Is Required for Floc Formation but Not for Extracellular Polysaccharide Biosynthesis in a Floc-Forming Aquincola tertiaricarbonis Strain

Journal Article · · Applied and Environmental Microbiology
DOI:https://doi.org/10.1128/AEM.00709-17· OSTI ID:1567087
 [1];  [1];  [2];  [2];  [1];  [3];  [4];  [5];  [6];  [2];  [4];  [5];  [2];  [7]
  1. Chinese Academy of Sciences, Wuhan (China); Univ. of Chinese Academy of Sciences, Beijing (China)
  2. Chinese Academy of Sciences, Wuhan (China)
  3. Univ. of Oklahoma, Norman, OK (United States); Henan Agricultural Univ., Zhengzhou (China)
  4. Chinese Academy of Sciences, Chengdu (China)
  5. Univ. of Oklahoma, Norman, OK (United States)
  6. Hubei Engineering Univ., Xiaogan (China)
  7. Univ. of Minnesota, Minneapolis, MN (United States)
+ Show Author Affiliations
Some bacteria are capable of forming flocs, in which bacterial cells become self-flocculated by secreted extracellular polysaccharides and other biopolymers. The floc-forming bacteria play a central role in activated sludge, which has been widely utilized for the treatment of municipal sewage and industrial wastewater. Here, we use a floc-forming bacterium, Aquincola tertiaricarbonis RN12, as a model to explore the biosynthesis of extracellular polysaccharides and the regulation of floc formation. A large gene cluster for exopolysaccharide biosynthesis and a gene encoding the alternative sigma factor RpoN1, one of the four paralogues, have been identified in floc formation-deficient mutants generated by transposon mutagenesis, and the gene functions have been further confirmed by genetic complementation analyses. Interestingly, the biosynthesis of exopolysaccharides remained in the rpoN1-disrupted flocculation-defective mutants, but most of the exopolysaccharides were secreted and released rather than bound to the cells. Furthermore, the expression of exopolysaccharide biosynthesis genes seemed not to be regulated by RpoN1. Taken together, our results indicate that RpoN1 may play a role in regulating the expression of a certain gene(σs) involved in the self-flocculation of bacterial cells but not in the biosynthesis and secretion of exopolysaccharides required for floc formation.
Research Organization:
Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)
Sponsoring Organization:
USDOE Office of Science (SC)
Grant/Contract Number:
AC02-05CH11231
OSTI ID:
1567087
Journal Information:
Applied and Environmental Microbiology, Journal Name: Applied and Environmental Microbiology Journal Issue: 14 Vol. 83; ISSN 0099-2240
Publisher:
American Society for MicrobiologyCopyright Statement
Country of Publication:
United States
Language:
English

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Cited By (2)

Pleiotropic control of antibiotic biosynthesis, flagellar operon expression, biofilm formation, and carbon source utilization by RpoN in Pseudomonas protegens H78 journal August 2018
Both widespread PEP-CTERM proteins and exopolysaccharides are required for floc formation of Zoogloea resiniphila and other activated sludge bacteria: PEP-CTERM protein biomarker for floc formation journal March 2018

Figures / Tables (9)

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TABLE 2(p. 19)table TABLE 2
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