Bacterial community and arsenic functional genes diversity in arsenic contaminated soils from different geographic locations

Gu, Yunfu; D. Van Nostrand, Joy; Wu, Liyou; He, Zhili; Qin, Yujia; Zhao, Fang-Jie; Zhou, Jizhong

doi:10.1371/journal.pone.0176696

Title: Bacterial community and arsenic functional genes diversity in arsenic contaminated soils from different geographic locations

Journal Article · Fri May 05 00:00:00 EDT 2017 · PLoS ONE

DOI:https://doi.org/10.1371/journal.pone.0176696· OSTI ID:1567072

Gu, Yunfu ^[1]; D. Van Nostrand, Joy ^[2]; Wu, Liyou ^[2]; He, Zhili ^[2]; Qin, Yujia ^[2]; Zhao, Fang-Jie ^[3]; Zhou, Jizhong ^[4]

Sichuan Agricultural Univ., Chengdu (China)
Univ. of Oklahoma, Norman, OK (United States)
Nanjing Agricultural Univ. (China); Rothamsted Research, Hertfordshire (United Kingdom)
Univ. of Oklahoma, Norman, OK (United States); Tsinghua Univ., Beijing (China); Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)

To understand how soil microbial communities and arsenic (As) functional genes respond to soil arsenic (As) contamination, five soils contaminated with As at different levels were collected from diverse geographic locations, incubated for 54 days under flooded conditions, and examined by both MiSeq sequencing of 16S rRNA gene amplicons and functional gene microarray (GeoChip 4.0). The results showed that both bacterial community structure and As functional gene structure differed among geographical locations. The diversity of As functional genes correlated positively with the diversity of 16S rRNA genes (P< 0.05). Higher diversities of As functional genes and 16S rRNA genes were observed in the soils with higher available As. Soil pH, phosphate-extractable As, and amorphous Fe content were the most important factors in shaping the bacterial community structure and As transformation functional genes. Geographic location was also important in controlling both the bacterial community and As transformation functional potential. These findings provide insights into the variation of As transformation functional genes in soils contaminated with different levels of As at different geographic locations, and the impact of environmental As contamination on the soil bacterial community.

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Research Organization:: Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)

Sponsoring Organization:: USDOE Office of Science (SC)

Grant/Contract Number:: AC02-05CH11231

OSTI ID:: 1567072

Journal Information:: PLoS ONE, Vol. 12, Issue 5; ISSN 1932-6203

Publisher:: Public Library of ScienceCopyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 46 works

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