The Biogeographic Pattern of Microbial Functional Genes along an Altitudinal Gradient of the Tibetan Pasture

Qi, Qi; Zhao, Mengxin; Wang, Shiping; Ma, Xingyu; Wang, Yuxuan; Gao, Ying; Lin, Qiaoyan; Li, Xiangzhen; Gu, Baohua; Li, Guoxue; Zhou, Jizhong; Yang, Yunfeng

doi:10.3389/fmicb.2017.00976

The Biogeographic Pattern of Microbial Functional Genes along an Altitudinal Gradient of the Tibetan Pasture

Journal Article · Tue Jun 13 00:00:00 EDT 2017 · Frontiers in Microbiology

DOI:https://doi.org/10.3389/fmicb.2017.00976· OSTI ID:1567071

Qi, Qi ^[1]; Zhao, Mengxin ^[2]; Wang, Shiping ^[3]; Ma, Xingyu ^[2]; Wang, Yuxuan ^[4]; Gao, Ying ^[2]; Lin, Qiaoyan ^[5]; Li, Xiangzhen ^[6]; Gu, Baohua ^[7]; Li, Guoxue ^[8]; Zhou, Jizhong ^[9]; Yang, Yunfeng ^[2]

Tsinghua Univ., Beijing (China); DOE/OSTI
Tsinghua Univ., Beijing (China)
Chinese Academy of Sciences (CAS), Beijing (China); CAS Center for Excellence in Tibetan Plateau Earth Science, Beijing (China)
Tsinghua Univ., Beijing (China); Univ. of Houston, TX (United States)
Chinese Academy of Sciences (CAS), Xining (China). Key Lab. of Adaptation and Evolution of Plateau Biota, Northwest Inst. of Plateau Biology
Chinese Academy of Sciences (CAS), Chengdu (China). Key Lab. of Environmental and Applied Microbiology, Environmental Microbiology Key Lab. of Sichuan Province, Chengdu Inst. of Biology
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
China Agricultural Univ., Beijing (China)
Tsinghua Univ., Beijing (China); Univ. of Oklahoma, Norman, OK (United States). Inst. for Environmental Genomics and Department of Microbiology and Plant Biology; Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)

As the highest place of the world, the Tibetan plateau is a fragile ecosystem. Given the importance of microbial communities in driving soil nutrient cycling, it is of interest to document the microbial biogeographic pattern here. We adopted a microarray-based tool named GeoChip 4.0 to investigate grassland microbial functional genes along an elevation gradient from 3200 to 3800 m above sea level open to free grazing by local herdsmen and wild animals. Interestingly, microbial functional diversities increase with elevation, so does the relative abundances of genes associated with carbon degradation, nitrogen cycling, methane production, cold shock and oxygen limitation. The range of Shannon diversities (10.27-10.58) showed considerably smaller variation than what was previously observed at ungrazed sites nearby (9.95-10.65), suggesting the important role of livestock grazing on microbial diversities. Closer examination showed that the dissimilarity of microbial community at our study sites increased with elevations, revealing an elevation-decay relationship of microbial functional genes. Both microbial functional diversity and the number of unique genes increased with elevations. Furthermore, we detected a tight linkage of greenhouse gas (CO₂) and relative abundances of carbon cycling genes. Our biogeographic study provides insights on microbial functional diversity and soil biogeochemical cycling in Tibetan pastures.

View Accepted Manuscript (DOE)

Research Organization:: Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)

Sponsoring Organization:: USDOE Office of Science (SC); National Science Foundation of China; Chinese Academy of Sciences

Grant/Contract Number:: AC02-05CH11231

OSTI ID:: 1567071

Journal Information:: Frontiers in Microbiology, Journal Name: Frontiers in Microbiology Journal Issue: JUN Vol. 8; ISSN 1664-302X

Publisher:: Frontiers Research FoundationCopyright Statement

Country of Publication:: United States

Language:: English

References (93)

Temperature Sensitivity and Soil Organic Carbon Pools Decomposition under Different Moisture Regimes: Effect on Total Microbial and Enzymatic Activity: Temperature Sensitivity and Soil Organic Carbon Pools Decomposition Hassan, Waseem; Bano, Rizwana; Khatak, Bashir Ullah CLEAN - Soil, Air, Water, Vol. 43, Issue 3 https://doi.org/10.1002/clen.201300727	journal	July 2014
Contrasting soil microbial community functional structures in two major landscapes of the Tibetan alpine meadow Chu, Houjuan; Wang, Shiping; Yue, Haowei MicrobiologyOpen, Vol. 3, Issue 5 https://doi.org/10.1002/mbo3.190	journal	July 2014
Soil Microbial Responses to Temporal Variations of Moisture and Temperature in a Chihuahuan Desert Grassland Bell, Colin; McIntyre, Nancy; Cox, Stephen Microbial Ecology, Vol. 56, Issue 1 https://doi.org/10.1007/s00248-007-9333-z	journal	February 2008
Diversity, Abundance, and Distribution of nirS-Harboring Denitrifiers in Intertidal Sediments of the Yangtze Estuary Zheng, Yanling; Hou, Lijun; Liu, Min Microbial Ecology, Vol. 70, Issue 1 https://doi.org/10.1007/s00248-015-0567-x	journal	January 2015
Similarities and Contrasts in the Archaeal Community of Two Japanese Mountains: Mt. Norikura Compared to Mt. Fuji Singh, Dharmesh; Takahashi, Koichi; Park, Jungok Microbial Ecology, Vol. 71, Issue 2 https://doi.org/10.1007/s00248-015-0681-9	journal	September 2015
Abundance and Diversity of Bacterial, Archaeal, and Fungal Communities Along an Altitudinal Gradient in Alpine Forest Soils: What Are the Driving Factors? Siles, José A.; Margesin, Rosa Microbial Ecology, Vol. 72, Issue 1 https://doi.org/10.1007/s00248-016-0748-2	journal	March 2016
The Microbiomes in Lichen and Moss Biocrust Contribute Differently to Carbon and Nitrogen Cycles in Arid Ecosystems Tian, Chang; Pang, Jingwen; Bu, Chongfeng Microbial Ecology, Vol. 86, Issue 1 https://doi.org/10.1007/s00248-022-02077-7	journal	July 2022
Diversity and distribution of autotrophic microbial community along environmental gradients in grassland soils on the Tibetan Plateau Guo, Guangxia; Kong, Weidong; Liu, Jinbo Applied Microbiology and Biotechnology, Vol. 99, Issue 20 https://doi.org/10.1007/s00253-015-6723-x	journal	June 2015
Soil Microbial Community Changes in Wooded Mountain Pastures due to Simulated Effects of Cattle Grazing Kohler, F.; Hamelin, J.; Gillet, F. Plant and Soil, Vol. 278, Issue 1-2 https://doi.org/10.1007/s11104-005-8809-1	journal	December 2005
Response of Ammonium-Oxidizing (amoA) and Nitrate-Reducing (narG) Gene Abundances in Groundwater to Land Use Change Qin, Hongling; Quan, Zhi; Yuan, Hongzhao Water, Air, & Soil Pollution, Vol. 225, Issue 5 https://doi.org/10.1007/s11270-014-1908-y	journal	April 2014
The accumulation and transfer of arsenic and mercury in the soil under a long-term fertilization treatment Wang, Qingyun; Zhang, Jiabao; Xin, Xiuli Journal of Soils and Sediments, Vol. 16, Issue 2 https://doi.org/10.1007/s11368-015-1227-y	journal	August 2015
Phylogenetic identification and microbial diversity in snow of the summit (8201 m) of Cho Oyu Mountain, Tibet Tong, XiaoMei; Chen, Fang; Yu, Jun Science Bulletin, Vol. 53, Issue 21 https://doi.org/10.1007/s11434-008-0424-0	journal	October 2008
Short-term carbon and nitrogen cycling in urine patches assessed by combined carbon-13 and nitrogen-15 labelling Ambus, P.; Petersen, S. O.; Soussana, J. -F. Agriculture, Ecosystems & Environment, Vol. 121, Issue 1-2 https://doi.org/10.1016/j.agee.2006.12.007	journal	June 2007
Effects of litter addition and warming on soil carbon, nutrient pools and microbial communities in a subarctic heath ecosystem Rinnan, Riikka; Michelsen, Anders; Jonasson, Sven Applied Soil Ecology, Vol. 39, Issue 3 https://doi.org/10.1016/j.apsoil.2007.12.014	journal	July 2008
Effect of land-use and elevation on microbial biomass and water extractable carbon in soils of Mt. Kilimanjaro ecosystems Pabst, Holger; Kühnel, Anna; Kuzyakov, Yakov Applied Soil Ecology, Vol. 67 https://doi.org/10.1016/j.apsoil.2013.02.006	journal	May 2013
Testing for differences in beta diversity with asymmetric dissimilarities Ricotta, Carlo; Burrascano, Sabina Ecological Indicators, Vol. 9, Issue 4 https://doi.org/10.1016/j.ecolind.2008.09.003	journal	July 2009
Spatial variability of soil properties affected by grazing intensity in Inner Mongolia grassland Zhao, Ying; Peth, Stephan; Krümmelbein, Julia Ecological Modelling, Vol. 205, Issue 1-2 https://doi.org/10.1016/j.ecolmodel.2007.02.019	journal	July 2007
Effects of soil moisture and plant interactions on the soil microbial community structure Chen, M. -M.; Zhu, Y. -G.; Su, Y. -H. European Journal of Soil Biology, Vol. 43, Issue 1 https://doi.org/10.1016/j.ejsobi.2006.05.001	journal	January 2007
Distribution and elevated soil pools of mercury in an acidic subtropical forest of southwestern China Zhou, Jun; Wang, Zhangwei; Zhang, Xiaoshan Environmental Pollution, Vol. 202 https://doi.org/10.1016/j.envpol.2015.03.021	journal	July 2015
Soil organic carbon stocks in relation to elevation gradients in volcanic ash soils of Taiwan Tsui, Chun-Chih; Tsai, Chen-Chi; Chen, Zueng-Sang Geoderma, Vol. 209-210 https://doi.org/10.1016/j.geoderma.2013.06.013	journal	November 2013
Livestock grazing modifies the effect of environmental factors on soil temperature and water content in a temperate grassland Odriozola, I.; García-Baquero, G.; Laskurain, N. A. Geoderma, Vol. 235-236 https://doi.org/10.1016/j.geoderma.2014.08.002	journal	December 2014
Significance of Detrended Correspondence Analysis (DCA) in palaeoecology and biostratigraphy: A case study from the Upper Cretaceous of Egypt Ayoub-Hannaa, W.; Huntley, J. W.; Fürsich, F. T. Journal of African Earth Sciences, Vol. 80 https://doi.org/10.1016/j.jafrearsci.2012.11.012	journal	April 2013
Temporal shifts in diversity and quantity of nirS and nirK in a rice paddy field soil Yoshida, Megumi; Ishii, Satoshi; Otsuka, Shigeto Soil Biology and Biochemistry, Vol. 41, Issue 10 https://doi.org/10.1016/j.soilbio.2009.07.012	journal	October 2009
Effects of warming and grazing on N2O fluxes in an alpine meadow ecosystem on the Tibetan plateau Hu, Yigang; Chang, Xiaofeng; Lin, Xingwu Soil Biology and Biochemistry, Vol. 42, Issue 6 https://doi.org/10.1016/j.soilbio.2010.02.011	journal	June 2010
Strong elevational trends in soil bacterial community composition on Mt. Halla, South Korea Singh, Dharmesh; Lee-Cruz, Larisa; Kim, Woo-Sung Soil Biology and Biochemistry, Vol. 68 https://doi.org/10.1016/j.soilbio.2013.09.027	journal	January 2014
An inventory of heavy metals inputs to agricultural soils in England and Wales Nicholson, F. A.; Smith, S. R.; Alloway, B. J. Science of The Total Environment, Vol. 311, Issue 1-3 https://doi.org/10.1016/s0048-9697(03)00139-6	journal	July 2003
China: The third pole Qiu, Jane Nature, Vol. 454, Issue 7203 https://doi.org/10.1038/454393a	journal	July 2008
GeoChip: a comprehensive microarray for investigating biogeochemical, ecological and environmental processes He, Zhili; Gentry, Terry J.; Schadt, Christopher W. The ISME Journal, Vol. 1, Issue 1 https://doi.org/10.1038/ismej.2007.2	journal	May 2007
Bacterial gene abundances as indicators of greenhouse gas emission in soils Morales, Sergio E.; Cosart, Theodore; Holben, William E. The ISME Journal, Vol. 4, Issue 6 https://doi.org/10.1038/ismej.2010.8	journal	February 2010
Linking activity, composition and seasonal dynamics of atmospheric methane oxidizers in a meadow soil Shrestha, Pravin Malla; Kammann, Claudia; Lenhart, Katharina The ISME Journal, Vol. 6, Issue 6 https://doi.org/10.1038/ismej.2011.179	journal	December 2011
Erratum: Elevated nitrate enriches microbial functional genes for potential bioremediation of complexly contaminated sediments Xu, Meiying; Zhang, Qin; Xia, Chunyu The ISME Journal, Vol. 9, Issue 2 https://doi.org/10.1038/ismej.2014.229	journal	January 2015
Relationships between protein-encoding gene abundance and corresponding process are commonly assumed yet rarely observed Rocca, Jennifer D.; Hall, Edward K.; Lennon, Jay T. The ISME Journal, Vol. 9, Issue 8 https://doi.org/10.1038/ismej.2014.252	journal	December 2014
Microbial mediation of biogeochemical cycles revealed by simulation of global changes with soil transplant and cropping Zhao, Mengxin; Xue, Kai; Wang, Feng The ISME Journal, Vol. 8, Issue 10 https://doi.org/10.1038/ismej.2014.46	journal	April 2014
The microbe-mediated mechanisms affecting topsoil carbon stock in Tibetan grasslands Yue, Haowei; Wang, Mengmeng; Wang, Shiping The ISME Journal, Vol. 9, Issue 9 https://doi.org/10.1038/ismej.2015.19	journal	February 2015
Microbial regulation of the soil carbon cycle: evidence from gene–enzyme relationships Trivedi, Pankaj; Delgado-Baquerizo, Manuel; Trivedi, Chanda The ISME Journal, Vol. 10, Issue 11 https://doi.org/10.1038/ismej.2016.65	journal	May 2016
Belowground biodiversity and ecosystem functioning Bardgett, Richard D.; van der Putten, Wim H. Nature, Vol. 515, Issue 7528 https://doi.org/10.1038/nature13855	journal	November 2014
Ecological stability in response to warming Fussmann, Katarina E.; Schwarzmüller, Florian; Brose, Ulrich Nature Climate Change, Vol. 4, Issue 3 https://doi.org/10.1038/nclimate2134	journal	February 2014
Microbial diversity drives multifunctionality in terrestrial ecosystems Delgado-Baquerizo, Manuel; Maestre, Fernando T.; Reich, Peter B. Nature Communications, Vol. 7, Issue 1 https://doi.org/10.1038/ncomms10541	journal	January 2016
Beyond biogeographic patterns: processes shaping the microbial landscape Hanson, China A.; Fuhrman, Jed A.; Horner-Devine, M. Claire Nature Reviews Microbiology, Vol. 10, Issue 7 https://doi.org/10.1038/nrmicro2795	journal	May 2012
Integrated metagenomics and network analysis of soil microbial community of the forest timberline Ding, Junjun; Zhang, Yuguang; Deng, Ye Scientific Reports, Vol. 5, Issue 1 https://doi.org/10.1038/srep07994	journal	January 2015
Spatial scaling of functional gene diversity across various microbial taxa Zhou, J.; Kang, S.; Schadt, C. W. Proceedings of the National Academy of Sciences, Vol. 105, Issue 22 https://doi.org/10.1073/pnas.0709016105	journal	May 2008
PicoGreen quantitation of DNA: effective evaluation of samples pre- or post-PCR Ahn, S. Nucleic Acids Research, Vol. 24, Issue 13 https://doi.org/10.1093/nar/24.13.2623	journal	July 1996
Microbes follow Humboldt: temperature drives plant and soil microbial diversity patterns from the Amazon to the Andes Nottingham, Andrew T.; Fierer, Noah; Turner, Benjamin L. https://doi.org/10.1101/079996	posted_content	February 2018
The interactive effects of soil transplant into colder regions and cropping on soil microbiology and biogeochemistry: Effects of northward soil transplant and cropping Liu, Shanshan; Wang, Feng; Xue, Kai Environmental Microbiology, Vol. 17, Issue 3 https://doi.org/10.1111/1462-2920.12398	journal	February 2014
Soil bacterial diversity patterns and drivers along an elevational gradient on Shennongjia Mountain, China: Soil bacterial elevational pattern Zhang, Yuguang; Cong, Jing; Lu, Hui Microbial Biotechnology, Vol. 8, Issue 4 https://doi.org/10.1111/1751-7915.12288	journal	May 2015
Plant diversity predicts beta but not alpha diversity of soil microbes across grasslands worldwide Prober, Suzanne M.; Leff, Jonathan W.; Bates, Scott T. Ecology Letters, Vol. 18, Issue 1 https://doi.org/10.1111/ele.12381	journal	November 2014
Responses of the functional structure of soil microbial community to livestock grazing in the Tibetan alpine grassland Yang, Yunfeng; Wu, Linwei; Lin, Qiaoyan Global Change Biology, Vol. 19, Issue 2 https://doi.org/10.1111/gcb.12065	journal	November 2012
Effects of grazing on plant community structure and aboveground net primary production of semiarid boreal steppe of northern Mongolia Lkhagva, Ariuntsetseg; Boldgiv, Bazartseren; Goulden, Clyde E. Grassland Science, Vol. 59, Issue 3 https://doi.org/10.1111/grs.12022	journal	July 2013
Diurnal, seasonal and annual variation in net ecosystem CO ₂ exchange of an alpine shrubland on Qinghai-Tibetan plateau Zhao, Liang; Li, Yingnian; Xu, Shixiao Global Change Biology, Vol. 12, Issue 10 https://doi.org/10.1111/j.1365-2486.2006.01197.x	journal	October 2006
GeoChip-based analysis of functional microbial communities during the reoxidation of a bioreduced uranium-contaminated aquifer Van Nostrand, Joy D.; Wu, Wei-Min; Wu, Liyou Environmental Microbiology, Vol. 11, Issue 10 https://doi.org/10.1111/j.1462-2920.2009.01986.x	journal	October 2009
Evolutionary ecology of plant-microbe interactions: soil microbial structure alters selection on plant traits Lau, Jennifer A.; Lennon, Jay T. New Phytologist, Vol. 192, Issue 1 https://doi.org/10.1111/j.1469-8137.2011.03790.x	journal	June 2011
Response of Polygonum viviparum Species and Community Level to Long-term Livestock Grazing in Alpine Shrub Meadow in Qinghai-Tibet Plateau Zhu, Zhi-Hong; Lundholm, Jeremy; Li, Yingnian Journal of Integrative Plant Biology, Vol. 50, Issue 6 https://doi.org/10.1111/j.1744-7909.2008.00676.x	journal	June 2008
Ecological Linkages Between Aboveground and Belowground Biota Wardle, D. A. Science, Vol. 304, Issue 5677 https://doi.org/10.1126/science.1094875	journal	June 2004
The Microbial Engines That Drive Earth's Biogeochemical Cycles Falkowski, P. G.; Fenchel, T.; Delong, E. F. Science, Vol. 320, Issue 5879 https://doi.org/10.1126/science.1153213	journal	May 2008
Microbial Biogeography: From Taxonomy to Traits Green, J. L.; Bohannan, B. J. M.; Whitaker, R. J. Science, Vol. 320, Issue 5879 https://doi.org/10.1126/science.1153475	journal	May 2008
Diversity, Physiology, and Niche Differentiation of Ammonia-Oxidizing Archaea Hatzenpichler, Roland Applied and Environmental Microbiology, Vol. 78, Issue 21 https://doi.org/10.1128/aem.01960-12	journal	August 2012
Influence of Soil Characteristics on the Diversity of Bacteria in the Southern Brazilian Atlantic Forest Faoro, H.; Alves, A. C.; Souza, E. M. Applied and Environmental Microbiology, Vol. 76, Issue 14 https://doi.org/10.1128/aem.03025-09	journal	May 2010
Soil Microbial Responses to Increased Moisture and Organic Resources along a Salinity Gradient in a Polar Desert Van Horn, David J.; Okie, Jordan G.; Buelow, Heather N. Applied and Environmental Microbiology, Vol. 80, Issue 10 https://doi.org/10.1128/aem.03414-13	journal	March 2014
Development of PCR Primer Systems for Amplification of Nitrite Reductase Genes (nirK and nirS) To Detect Denitrifying Bacteria in Environmental Samples Braker, Gesche; Fesefeldt, Andreas; Witzel, Karl-Paul Applied and Environmental Microbiology, Vol. 64, Issue 10 https://doi.org/10.1128/aem.64.10.3769-3775.1998	journal	October 1998
Molecular Diversity of Denitrifying Genes in Continental Margin Sediments within the Oxygen-Deficient Zone off the Pacific Coast of Mexico Liu, X.; Tiquia, S. M.; Holguin, G. Applied and Environmental Microbiology, Vol. 69, Issue 6 https://doi.org/10.1128/aem.69.6.3549-3560.2003	journal	June 2003
Biphasic Behavior of Anammox Regulated by Nitrite and Nitrate in an Estuarine Sediment Trimmer, M.; Nicholls, J. C.; Morley, N. Applied and Environmental Microbiology, Vol. 71, Issue 4 https://doi.org/10.1128/aem.71.4.1923-1930.2005	journal	April 2005
Characterization of cspB, a Bacillus subtilis inducible cold shock gene affecting cell viability at low temperatures. Willimsky, G.; Bang, H.; Fischer, G. Journal of Bacteriology, Vol. 174, Issue 20 https://doi.org/10.1128/jb.174.20.6326-6335.1992	journal	October 1992
Phylogenetic diversity of bacteria isolates from the Qinghai-Tibet Plateau permafrost region Zhang, Gaosen; Niu, Fujun; Ma, Xiaojun Canadian Journal of Microbiology, Vol. 53, Issue 8 https://doi.org/10.1139/w07-031	journal	August 2007
Response of Soil Respiration to Grazing in an Alpine Meadow at Three Elevations in Tibet Fu, Gang; Zhang, Xianzhou; Yu, Chengqun The Scientific World Journal, Vol. 2014 https://doi.org/10.1155/2014/265142	journal	January 2014
Molecular ecological network analyses Deng, Ye; Jiang, Yi-Huei; Yang, Yunfeng BMC Bioinformatics, Vol. 13, Issue 1 https://doi.org/10.1186/1471-2105-13-113	journal	January 2012
Elevational Patterns in Archaeal Diversity on Mt. Fuji Singh, Dharmesh; Takahashi, Koichi; Adams, Jonathan M. PLoS ONE, Vol. 7, Issue 9 https://doi.org/10.1371/journal.pone.0044494	journal	September 2012
Microbes do not follow the elevational diversity patterns of plants and animals Fierer, Noah; McCain, Christy M.; Meir, Patrick Ecology, Vol. 92, Issue 4 https://doi.org/10.1890/10-1170.1	journal	April 2011
Variations of Soil Microbial Community Structures Beneath Broadleaved Forest Trees in Temperate and Subtropical Climate Zones Yang, Sihang; Zhang, Yuguang; Cong, Jing Frontiers in Microbiology, Vol. 8 https://doi.org/10.3389/fmicb.2017.00200	journal	February 2017
Quick measurement of CH4, CO2 and N2O emissions from a short-plant ecosystem Yuesi, Wang; Yinghong, Wang Advances in Atmospheric Sciences, Vol. 20, Issue 5 https://doi.org/10.1007/BF02915410	journal	September 2003
An inventory of heavy metals inputs to agricultural soils in England and Wales Nicholson, F. A.; Smith, S. R.; Alloway, B. J. Science of The Total Environment, Vol. 311, Issue 1-3 https://doi.org/10.1016/S0048-9697(03)00139-6	journal	July 2003
Livestock grazing, plateau pikas and the conservation of avian biodiversity on the Tibetan plateau Arthur, Anthony D.; Pech, Roger P.; Davey, Chris Biological Conservation, Vol. 141, Issue 8 https://doi.org/10.1016/j.biocon.2008.05.010	journal	August 2008
Long-term successional dynamics of microbial association networks in anaerobic digestion processes Wu, Linwei; Yang, Yunfeng; Chen, Si Water Research, Vol. 104 https://doi.org/10.1016/j.watres.2016.07.072	journal	November 2016
Field and laboratory studies of methane oxidation in an anoxic marine sediment: Evidence for a methanogen-sulfate reducer consortium Hoehler, Tori M.; Alperin, Marc J.; Albert, Daniel B. Global Biogeochemical Cycles, Vol. 8, Issue 4 https://doi.org/10.1029/94GB01800	journal	December 1994
Effect of warming and drought on grassland microbial communities Sheik, Cody S.; Beasley, William Howard; Elshahed, Mostafa S. The ISME Journal, Vol. 5, Issue 10 https://doi.org/10.1038/ismej.2011.32	journal	March 2011
The microbial gene diversity along an elevation gradient of the Tibetan grassland Yang, Yunfeng; Gao, Ying; Wang, Shiping The ISME Journal, Vol. 8, Issue 2 https://doi.org/10.1038/ismej.2013.146	journal	August 2013
Elevated nitrate enriches microbial functional genes for potential bioremediation of complexly contaminated sediments Xu, Meiying; Zhang, Qin; Xia, Chunyu The ISME Journal, Vol. 8, Issue 9 https://doi.org/10.1038/ismej.2014.42	journal	March 2014
Distribution of vascular plant species richness and endemic richness along the Himalayan elevation gradient in Nepal: Diversity along the Himalayan elevation gradient Vetaas, Ole R.; Grytnes, John-Arvid Global Ecology and Biogeography, Vol. 11, Issue 4 https://doi.org/10.1046/j.1466-822X.2002.00297.x	journal	July 2002
Microbes on mountainsides: Contrasting elevational patterns of bacterial and plant diversity Bryant, J. A.; Lamanna, C.; Morlon, H. Proceedings of the National Academy of Sciences, Vol. 105, Issue Supplement 1 https://doi.org/10.1073/pnas.0801920105	journal	August 2008
Acidification of Surface Soil in Croplands in the Semiarid Middle Tibet Plateau, China Zhou, Zhonghao; Du, Shuhan; Liu, Gangcai 2009 International Conference on Environmental Science and Information Application Technology, ESIAT https://doi.org/10.1109/ESIAT.2009.72	conference	July 2009
Pyrosequencing-Based Assessment of Soil pH as a Predictor of Soil Bacterial Community Structure at the Continental Scale Lauber, C. L.; Hamady, M.; Knight, R. Applied and Environmental Microbiology, Vol. 75, Issue 15 https://doi.org/10.1128/AEM.00335-09	journal	June 2009
Diversity, Physiology, and Niche Differentiation of Ammonia-Oxidizing Archaea Hatzenpichler, Roland Applied and Environmental Microbiology, Vol. 78, Issue 21 https://doi.org/10.1128/AEM.01960-12	journal	August 2012
Influence of Soil Characteristics on the Diversity of Bacteria in the Southern Brazilian Atlantic Forest Faoro, H.; Alves, A. C.; Souza, E. M. Applied and Environmental Microbiology, Vol. 76, Issue 14 https://doi.org/10.1128/AEM.03025-09	journal	May 2010
Soil Microbial Responses to Increased Moisture and Organic Resources along a Salinity Gradient in a Polar Desert Van Horn, David J.; Okie, Jordan G.; Buelow, Heather N. Applied and Environmental Microbiology, Vol. 80, Issue 10 https://doi.org/10.1128/AEM.03414-13	journal	March 2014
Development of PCR Primer Systems for Amplification of Nitrite Reductase Genes (nirK and nirS) To Detect Denitrifying Bacteria in Environmental Samples Braker, Gesche; Fesefeldt, Andreas; Witzel, Karl-Paul Applied and Environmental Microbiology, Vol. 64, Issue 10 https://doi.org/10.1128/AEM.64.10.3769-3775.1998	journal	October 1998
Molecular Diversity of Denitrifying Genes in Continental Margin Sediments within the Oxygen-Deficient Zone off the Pacific Coast of Mexico Liu, X.; Tiquia, S. M.; Holguin, G. Applied and Environmental Microbiology, Vol. 69, Issue 6 https://doi.org/10.1128/AEM.69.6.3549-3560.2003	journal	June 2003
Characterization of C1-Metabolizing Prokaryotic Communities in Methane Seep Habitats at the Kuroshima Knoll, Southern Ryukyu Arc, by Analyzing pmoA, mmoX, mxaF, mcrA, and 16S rRNA Genes Inagaki, F.; Tsunogai, U.; Suzuki, M. Applied and Environmental Microbiology, Vol. 70, Issue 12 https://doi.org/10.1128/AEM.70.12.7445-7455.2004	journal	December 2004
Biphasic Behavior of Anammox Regulated by Nitrite and Nitrate in an Estuarine Sediment Trimmer, M.; Nicholls, J. C.; Morley, N. Applied and Environmental Microbiology, Vol. 71, Issue 4 https://doi.org/10.1128/AEM.71.4.1923-1930.2005	journal	April 2005
High-Throughput Metagenomic Technologies for Complex Microbial Community Analysis: Open and Closed Formats Zhou, Jizhong; He, Zhili; Yang, Yunfeng mBio, Vol. 6, Issue 1 https://doi.org/10.1128/mBio.02288-14	journal	January 2015
Phylogenetic diversity of bacteria isolates from the Qinghai-Tibet Plateau permafrost region Zhang, Gaosen; Niu, Fujun; Ma, Xiaojun Canadian Journal of Microbiology, Vol. 53, Issue 8 https://doi.org/10.1139/W07-031	journal	August 2007
An Introduction to the Bootstrap Efron, Bradley; Tibshirani, R. J. Monographs on Statistics and Applied Probability https://doi.org/10.1201/9780429246593	book	May 1994
Dissimilatory Nitrate Reduction to Ammonium in Upland Tropical Forest Soils Silver, Whendee L.; Herman, Donald J.; Firestone, Mary K. Ecology, Vol. 82, Issue 9 https://doi.org/10.1890/0012-9658(2001)082[2410:DNRTAI]2.0.CO;2	journal	September 2001
Comparison of soil greenhouse gas fluxes from extensive and intensive grazing in a temperate maritime climate Skiba, U.; Jones, S. K.; Drewer, J. Biogeosciences, Vol. 10, Issue 2 https://doi.org/10.5194/bg-10-1231-2013	journal	January 2013
Microbes do not follow the elevational diversity patterns of plants and animals Fierer, Noah; McCain, Christy M.; Meir, Patrick Figshare https://doi.org/10.6084/m9.figshare.c.3303873.v1	collection	January 2016

Cited By (2)

Hierarchical drivers of soil microbial community structure variability in “Monte Perdido” Massif (Central Pyrenees) Jiménez, Juan J.; Igual, José M.; Villar, Luis Scientific Reports, Vol. 9, Issue 1 https://doi.org/10.1038/s41598-019-45372-z	journal	June 2019
Climate mediates continental scale patterns of stream microbial functional diversity Picazo, Félix; Vilmi, Annika; Aalto, Juha Microbiome, Vol. 8, Issue 1 https://doi.org/10.1186/s40168-020-00873-2	journal	June 2020

Figures / Tables (5)

Similar Records

The Biogeographic Pattern of Microbial Functional Genes along an Altitudinal Gradient of the Tibetan Pasture

Journal Article · Tue Jun 13 00:00:00 EDT 2017 · Frontiers in Microbiology · OSTI ID:1394543

Evaluating the lingering effect of livestock grazing on functional potentials of microbial communities in Tibetan grassland soils

Journal Article · Tue Apr 19 00:00:00 EDT 2016 · Plant and Soil · OSTI ID:1567066

The microbe-mediated mechanisms affecting topsoil carbon stock in Tibetan grasslands

Journal Article · Mon Feb 16 23:00:00 EST 2015 · The ISME Journal · OSTI ID:1225223

Related Subjects

altitudinal gradient
54 ENVIRONMENTAL SCIENCES
alpine grassland
microbial biogeography
microbial functional potential
soil microbial community

The Biogeographic Pattern of Microbial Functional Genes along an Altitudinal Gradient of the Tibetan Pasture

Citation Formats

References (93)

Cited By (2)

Figures / Tables (5)

Similar Records

Related Subjects