Wetland Sediments Host Diverse Microbial Taxa Capable of Cycling Alcohols

Martins, Paula Dalcin; Frank, Jeroen; Mitchell, Hugh D.; Markillie, Lye Meng; Wilkins, Michael J.; Stabb, Eric V.

doi:10.1128/AEM.00189-19

Wetland Sediments Host Diverse Microbial Taxa Capable of Cycling Alcohols

Journal Article · Thu May 30 00:00:00 EDT 2019 · Applied and Environmental Microbiology

DOI:https://doi.org/10.1128/AEM.00189-19· OSTI ID:1567060

^[1]; Frank, Jeroen ^[1]; Mitchell, Hugh D. ^[2]; Markillie, Lye Meng ^[2]; Wilkins, Michael J. ^[3]; Stabb, Eric V. ^[4]

Radboud Univ. Nijmegen, Nijmegen (The Netherlands)
Pacific Northwest National Lab. (PNNL), Richland, WA (United States)
Colorado State Univ., Fort Collins, CO (United States)
Univ. of Georgia, Athens, GA (United States)

Alcohols are commonly derived from the degradation of organic matter and yet are rarely measured in environmental samples. Wetlands in the Prairie Pothole Region (PPR) support extremely high methane emissions and the highest sulfate reduction rates reported to date, likely contributing to a significant proportion of organic matter mineralization in this system. Here, while ethanol and isopropanol concentrations up to 4 to 5 mM in PPR wetland pore fluids have been implicated in sustaining these high rates of microbial activity, the mechanisms that support alcohol cycling in this ecosystem are poorly understood. We leveraged metagenomic and transcriptomic tools to identify genes, pathways, and microorganisms potentially accounting for alcohol cycling in PPR wetlands. Phylogenetic analyses revealed diverse alcohol dehydrogenases and putative substrates. Alcohol dehydrogenase and aldehyde dehydrogenase genes were included in 62 metagenome-assembled genomes (MAGs) affiliated with 16 phyla.

View Accepted Manuscript (DOE)

Research Organization:: Pacific Northwest National Laboratory (PNNL), Richland, WA (United States)

Sponsoring Organization:: USDOE

Grant/Contract Number:: AC05-76RL01830

OSTI ID:: 1567060

Report Number(s):: PNNL-SA--147002

Journal Information:: Applied and Environmental Microbiology, Journal Name: Applied and Environmental Microbiology Journal Issue: 12 Vol. 85; ISSN 0099-2240

Publisher:: American Society for MicrobiologyCopyright Statement

Country of Publication:: United States

Language:: English

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