Hierarchically Nanostructured Hybrid Platform for Tumor Delineation and Image‐Guided Surgery via NIR‐II Fluorescence and PET Bimodal Imaging
- Molecular Imaging Program at Stanford (MIPS) Canary Center at Stanford for Cancer Early Detection Department of Radiology and Bio‐X Program School of Medicine Stanford University Stanford CA 94305‐5484 USA, Department of Pharmaceutics School of Pharmacy Nanjing Medical University Nanjing 211166 China
- Molecular Imaging Program at Stanford (MIPS) Canary Center at Stanford for Cancer Early Detection Department of Radiology and Bio‐X Program School of Medicine Stanford University Stanford CA 94305‐5484 USA, Department of Nuclear Medicine West China Hospital Sichuan University Chengdu 610041 China
- Molecular Imaging Program at Stanford (MIPS) Canary Center at Stanford for Cancer Early Detection Department of Radiology and Bio‐X Program School of Medicine Stanford University Stanford CA 94305‐5484 USA
- Molecular Imaging Program at Stanford (MIPS) Canary Center at Stanford for Cancer Early Detection Department of Radiology and Bio‐X Program School of Medicine Stanford University Stanford CA 94305‐5484 USA, State Key Laboratory of Electroanalytical Chemistry Changchun Institute of Applied Chemistry Chinese Academy of Sciences Changchun 130022 China
- Department of Pharmaceutics School of Pharmacy Nanjing Medical University Nanjing 211166 China
- Department of Colorectal Surgery The First Affiliated Hospital of Nanjing Medical University Nanjing 210029 China
- Key Laboratory for Organic Electronics and Information Displays and Jiangsu Key Laboratory for Biosensors Institute of Advanced Materials (IAM) Jiangsu National Synergetic Innovation Center for Advanced Materials (SICAM) Nanjing University of Posts and Telecommunications Nanjing 210023 China
- Nuclear Medicine and Molecular Imaging Key Laboratory of Sichuan Province Department of Nuclear Medicine The Affiliated Hospital of Southwest Medical University Luzhou 646000 China
Abstract Bimodal imaging with fluorescence in the second near infrared window (NIR‐II) and positron emission tomography (PET) has important significance for tumor diagnosis and management because of complementary advantages. It remains challenging to develop NIR‐II/PET bimodal probes with high fluorescent brightness. Herein, bioinspired nanomaterials (melanin dot, mesoporous silica nanoparticle, and supported lipid bilayer), NIR‐II dye CH‐4T, and PET radionuclide 64 Cu are integrated into a hybrid NIR‐II/PET bimodal nanoprobe. The resultant nanoprobe exhibits attractive properties such as highly uniform tunable size, effective payload encapsulation, high stability, dispersibility, and biocompatibility. Interestingly, the incorporation of CH‐4T into the nanoparticle leads to 4.27‐fold fluorescence enhancement, resulting in brighter NIR‐II imaging for phantoms in vitro and in situ. Benefiting from the fluorescence enhancement, NIR‐II imaging with the nanoprobe is carried out to precisely delineate and resect tumors. Additionally, the nanoprobe is successfully applied in tumor PET imaging, showing the accumulation of the nanoprobe in a tumor with a clear contrast from 2 to 24 h postinjection. Overall, this hierarchically nanostructured platform is able to dramatically enhance fluorescent brightness of NIR‐II dye, detect tumors with NIR‐II/PET imaging, and guide intraoperative resection. The NIR‐II/PET bimodal nanoprobe has high potential for sensitive preoperative tumor diagnosis and precise intraoperative image‐guided surgery.
- Sponsoring Organization:
- USDOE
- Grant/Contract Number:
- DE‐SC0008397
- OSTI ID:
- 1566189
- Journal Information:
- Small, Journal Name: Small Vol. 15 Journal Issue: 45; ISSN 1613-6810
- Publisher:
- Wiley Blackwell (John Wiley & Sons)Copyright Statement
- Country of Publication:
- Germany
- Language:
- English
Web of Science
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