Structure and function of the Orc1 BAH-nucleosome complex

De Ioannes, Pablo; Leon, Victor A.; Kuang, Zheng; Wang, Miao; Boeke, Jef D.; Hochwagen, Andreas; Armache, Karim-Jean

doi:10.1038/s41467-019-10609-y

Structure and function of the Orc1 BAH-nucleosome complex

Journal Article · Mon Jul 01 00:00:00 EDT 2019 · Nature Communications

DOI:https://doi.org/10.1038/s41467-019-10609-y· OSTI ID:1562715

^[1]; Leon, Victor A. ^[2]; ^[3]; ^[4]; ^[5]; ^[2]; Armache, Karim-Jean ^[4]

New York Univ. (NYU), NY (United States). School of Medicine; DOE/OSTI
New York Univ. (NYU), NY (United States)
Univ. of Texas Southwestern Medical Center, Dallas, TX (United States); New York University (NYU) Langone Health, NY (United States)
New York Univ. (NYU), NY (United States). School of Medicine
New York University (NYU) Langone Health, NY (United States)

The Origin Recognition Complex (ORC) is essential for replication, heterochromatin formation, telomere maintenance and genome stability in eukaryotes. Here we present the structure of the yeast Orc1 BAH domain bound to the nucleosome core particle. Our data reveal that Orc1, unlike its close homolog Sir3 involved in gene silencing, does not appear to discriminate between acetylated and non-acetylated lysine 16, modification states of the histone H4 tail that specify open and closed chromatin respectively. We elucidate the mechanism for this unique feature of Orc1 and hypothesize that its ability to interact with nucleosomes regardless of K16 modification state enables it to perform critical functions in both hetero- and euchromatin. We also show that direct interactions with nucleosomes are essential for Orc1 to maintain the integrity of rDNA borders during meiosis, a process distinct and independent from its known roles in silencing and replication.

View Accepted Manuscript (DOE)

Research Organization:: Argonne National Laboratory (ANL), Argonne, IL (United States). Advanced Photon Source (APS)

Sponsoring Organization:: David and Lucile Packard Foundation; NYU School of Medicine; National Institutes of Health (NIH); USDOE

Grant/Contract Number:: AC02-06CH11357

OSTI ID:: 1562715

Journal Information:: Nature Communications, Journal Name: Nature Communications Journal Issue: 1 Vol. 10; ISSN 2041-1723

Publisher:: Nature Publishing GroupCopyright Statement

Country of Publication:: United States

Language:: ENGLISH

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59 BASIC BIOLOGICAL SCIENCES
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nucleosomes
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