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Hexokinase is necessary for glucose-mediated photosynthesis repression and lipid accumulation in a green alga

Journal Article · · Communications Biology
 [1];  [2];  [2];  [2]
  1. Univ. of California, Berkeley, CA (United States). Howard Hughes Medical Inst., Dept. of Plant and Microbial Biology; Lawrence Berkeley National Lab., Berkeley, CA (United States). Molecular Biophysics and Integrated Bioimaging Division; University of California, Berkeley
  2. Univ. of California, Berkeley, CA (United States). Howard Hughes Medical Inst., Dept. of Plant and Microbial Biology; Lawrence Berkeley National Lab., Berkeley, CA (United States). Molecular Biophysics and Integrated Bioimaging Division
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Global primary production is driven largely by oxygenic photosynthesis, with algae as major contributors. The green alga Chromochloris zofingiensisreversibly switches off photosynthesis in the presence of glucose in the light and augments production of biofuel precursors(triacylglycerols) and the high-value antioxidant astaxanthin. Here we used forward genetics to reveal that this photosynthetic and metabolic switch is mediated by the glycolytic enzyme hexokinase (CzHXK1). In contrast to wild-type, glucose-treated hxk1 mutants do not shut off photosynthesis or accumulate astaxanthin, triacylglycerols, or cytoplasmic lipid droplets. We show that CzHXK1 is critical for the regulation of genes related to photosynthesis, ketocarotenoid synthesis and fatty acid biosynthesis. Sugars play fundamental regulatory roles in gene expression, physiology, metabolism, and growth in plants and animals, and we introduce a relatively simple, emerging model system to investigate conserved eukaryotic sugar sensing and signaling at the base of the green lineage.
Research Organization:
Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States); Univ. of California, Berkeley, CA (United States)
Sponsoring Organization:
USDOE Office of Science (SC), Biological and Environmental Research (BER) (SC-23); USDOE Office of Science (SC), Biological and Environmental Research (BER) (SC-23). Biological Systems Science Division
Grant/Contract Number:
AC02-05CH11231; SC0018301
OSTI ID:
1562686
Alternate ID(s):
OSTI ID: 1580873
Journal Information:
Communications Biology, Journal Name: Communications Biology Journal Issue: 1 Vol. 2; ISSN 2399-3642
Publisher:
Springer NatureCopyright Statement
Country of Publication:
United States
Language:
English

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