Determination of cis,cis- and cis,trans-Muconic Acid from Biological Conversion: Laboratory Analytical Procedure (LAP)
Muconic acid is an important platform molecule for production of bio-based polymers. To date, cis,cis-muconic acid (ccMA) has been produced from multiple types of carbon sources including carbohydrates and aromatic compounds via cultivation with genetically engineered microbes. Accurate analysis of muconic acid has previously been a challenge due to ccMA isomerization into cis,trans-muconic acid (ctMA). This Laboratory Analytical Procedure (LAP) is used to separately quantify ccMA and ctMA as the total sum of muconic acid present in the liquid fraction of pretreated biological samples.
- Research Organization:
- National Renewable Energy Laboratory (NREL), Golden, CO (United States)
- Sponsoring Organization:
- USDOE Office of Energy Efficiency and Renewable Energy (EERE), Office of Sustainable Transportation. Bioenergy Technologies Office (BETO)
- DOE Contract Number:
- AC36-08GO28308
- OSTI ID:
- 1561511
- Report Number(s):
- NREL/TP-5100-74473
- Country of Publication:
- United States
- Language:
- English
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