Increased adaxial stomatal density is associated with greater mesophyll surface area exposed to intercellular air spaces and mesophyll conductance in diverse C 4 grasses

Pathare, Varsha S.; Koteyeva, Nuria; Cousins, Asaph B.

doi:10.1111/nph.16106

Increased adaxial stomatal density is associated with greater mesophyll surface area exposed to intercellular air spaces and mesophyll conductance in diverse C ₄ grasses

Journal Article · Wed Sep 04 00:00:00 EDT 2019 · New Phytologist

DOI:https://doi.org/10.1111/nph.16106· OSTI ID:1560286

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School of Biological Sciences Washington State University Pullman WA 99164‐4236 USA
School of Biological Sciences Washington State University Pullman WA 99164‐4236 USA, Laboratory of Anatomy and Morphology V.L. Komarov Botanical Institute of the Russian Academy of Sciences 197376 St Petersburg Russia

Summary

Mesophyll conductance ( g _m ) is the diffusion of CO ₂ from intercellular air spaces ( IAS ) to the first site of carboxylation in the mesophyll cells. In C ₃ species, g _m is influenced by diverse leaf structural and anatomical traits; however, little is known about traits affecting g _m in C ₄ species.

To address this knowledge gap, we used online oxygen isotope discrimination measurements to estimate g _m and microscopy techniques to measure leaf structural and anatomical traits potentially related to g _m in 18 C ₄ grasses.

In this study, g _m scaled positively with photosynthesis and intrinsic water‐use efficiency ( TE _i ), but not with stomatal conductance. Also, g _m was not determined by a single trait but was positively correlated with adaxial stomatal densities ( SD _ada ), stomatal ratio ( SR ), mesophyll surface area exposed to IAS ( S _mes ) and leaf thickness. However, g _m was not related to abaxial stomatal densities ( SD _aba ) and mesophyll cell wall thickness ( T _CW ).

Our study suggests that greater SD _ada and SR increased g _m by increasing S _mes and creating additional parallel pathways for CO ₂ diffusion inside mesophyll cells. Thus, SD _ada , SR and S _mes are important determinants of C ₄ ‐ g _m and could be the target traits selected or modified for achieving greater g _m and TE _i in C ₄ species.

View Accepted Manuscript (Publisher)

Sponsoring Organization:: USDOE

Grant/Contract Number:: SC0001685

OSTI ID:: 1560286

Journal Information:: New Phytologist, Journal Name: New Phytologist Journal Issue: 1 Vol. 225; ISSN 0028-646X

Publisher:: Wiley-BlackwellCopyright Statement

Country of Publication:: United Kingdom

Language:: English

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Increased adaxial stomatal density is associated with greater mesophyll surface area exposed to intercellular air spaces and mesophyll conductance in diverse C ₄ grasses