Periodic Arrangement of Lipopolysaccharides Nanostructures Accelerates and Enhances the Maturation Processes of Dendritic Cells

Liu, Yang; Wang, Kang-Hsin; Chen, Huan-Yuan; Li, Jie-Ren; Laurence, Ted A.; Ly, Sonny; Liu, Fu-Tong; Liu, Gang-Yu

doi:10.1021/acsanm.7b00254

Title: Periodic Arrangement of Lipopolysaccharides Nanostructures Accelerates and Enhances the Maturation Processes of Dendritic Cells

Journal Article · Mon Jan 29 00:00:00 EST 2018 · ACS Applied Nano Materials

DOI:https://doi.org/10.1021/acsanm.7b00254· OSTI ID:1560109

Liu, Yang ^[1]; Wang, Kang-Hsin ^[2]; Chen, Huan-Yuan ^[3]; Li, Jie-Ren ^[1]; Laurence, Ted A. ^[4]; Ly, Sonny ^[4]; Liu, Fu-Tong ^[5];

^[1]

Univ. of California, Davis, CA (United States). Dept. of Chemistry
Univ. of California, Davis, CA (United States). Dept. of Chemistry and Dept. of Dermatology, School of Medicine
Univ. of California, Davis, CA (United States). Dept. of Dermatology, School of Medicine; Academia Sinica, Taipei, (Taiwan). Inst. of Biomedical Sciences
Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)
Univ. of California, Sacramento, CA (United States). Dept. of Dermatology, School of Medicine; Academia Sinica, Taipei, (Taiwan). Inst. of Biomedical Sciences

This work reports an important application of nanomaterials consisting of biological ligands, such as lipopolysaccharides (LPS) nanostructures. Upon immobilization onto surfaces as periodic arrays of nanostructures, these surface-bound LPS nanostructures significantly impacted the maturation process of bone marrow derived dendritic cells (BMDCs): accelerating the maturation process in comparison to other means of ligand presentations such as LPS solution and monolayers on surfaces. In some cases, LPS nanostructures led to a new maturation phenotype in vitro characterized by hyper-dendritized morphology. This level of maturation enhancement was observed for the first time in vitro. Possible mechanisms leading to the acceleration and enhancement of BMDC maturation were also discussed. This work demonstrates an important concept: surface-bound nanostructures of ligands provide a new and powerful cue for regulating cellular signaling processes. The fact that primary cells’ signaling processes, such as BMDC maturation, can be altered suggests a new means for programming dendritic cells for immune therapy.

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Research Organization:: Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)

Sponsoring Organization:: USDOE National Nuclear Security Administration (NNSA)

Grant/Contract Number:: AC52-07NA27344

OSTI ID:: 1560109

Report Number(s):: LLNL-JRNL-779339; 970006

Journal Information:: ACS Applied Nano Materials, Vol. 1, Issue 2; ISSN 2574-0970

Publisher:: American Chemical Society (ACS)Copyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 5 works

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