Multiresolution Localization with Temporal Scanning for Super-Resolution Diffuse Optical Imaging of Fluorescence

Journal Article · Mon Aug 12 00:00:00 EDT 2019 · IEEE Transactions on Image Processing

DOI:https://doi.org/10.1109/TIP.2019.2931080· OSTI ID:1559546

Bentz, Brian Z. ^[1]; Lin, Dergan ^[2]; Patel, Justin A. ^[2]; Webb, Kevin J. ^[2]

---

1. Sandia National Lab. (SNL-NM), Albuquerque, NM (United States)
2. Purdue Univ., West Lafayette, IN (United States)

A super-resolution optical imaging method is introduced that relies on the distinct temporal information associated with each fluorescent optical reporter to determine its spatial position to high precision with measurements of heavily scattered light. This multiple-emitter localization approach uses a diffusion equation forward model in a cost function, and has the potential to achieve micron-scale spatial resolution through centimeters of tissue. Utilizing some degree of temporal separation for the reporter emissions, position and emission strength are determined using a computationally efficient time stripping multiresolution algorithm. The method circumvents the spatial resolution challenges faced by earlier optical imaging approaches using a diffusion equation forward model, and is promising for in vivo applications. For example, in principle, the approach could be used to localize individual neurons firing throughout a rodent brain, enabling direct imaging of neural network activity.

• Research Organization: Sandia National Laboratories (SNL-NM), Albuquerque, NM (United States)
• Sponsoring Organization: National Institutes of Health (NIH); National Science Foundation (NSF); USDOE National Nuclear Security Administration (NNSA)
• Grant/Contract Number: AC04-94AL85000
• OSTI ID: 1559546
• Report Number(s): SAND--2018-10834J; 668347
• Journal Information: IEEE Transactions on Image Processing, Journal Name: IEEE Transactions on Image Processing Vol. 29; ISSN 1057-7149
• Publisher: IEEE
• Country of Publication: United States
• Language: English

Related Subjects

42 ENGINEERING
Super-resolution
fluorescence
localization
matching pursuit
optical imaging
turbid media